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• W2029201935 abstract "125I-omega-conotoxin binding to neuroblastoma cells at 37 degrees C continuously increased, reaching a plateau after 6-8 hr; this was up to 6 times higher than that observed at lower temperatures. The same effect was induced by short pulses with omega-conotoxin followed by a chase period at 37 degrees C in control medium. Cd2+ also induced up-regulation of surface 125I-omega-conotoxin-binding sites. Fura-2 and patch-clamp experiments showed that the recruited binding sites corresponded to functional voltage-operated Ca2+ channels. Permeabilization experiments revealed a large intracellular pool of 125I-omega-conotoxin-binding sites, whose recruitment to the plasmamembrane was prevented by brefeldin A and nocodazole. These data suggest that specific stimuli might induce voltage-operated Ca2+ channel translocation to plasmamembrane and, in this way, modulate presynaptic events." @default.
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• W2029201935 date "1994-02-01" @default.
• W2029201935 modified "2023-09-23" @default.
• W2029201935 title "ω-conotoxin and Cd2+ stimulate the recruitment to the plasmamembrane of an intracellular pool of voltage-operated Ca2+ channels" @default.
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• W2029201935 doi "https://doi.org/10.1016/0896-6273(94)90274-7" @default.
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