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- W2029206815 abstract "Buccal mucosal cancer (BMC) is a multifactorial disease with poorly defined genetic profile and prognosis due to late detection stage and unavailability of reliable prognostic markers. To identify aberrant transcriptional events, we employed high throughput RNA-Seq analysis of BMC and normal tissue. Comparative transcriptome analysis with Cufflinks revealed 260 up and 328 down regulated genes whereas, 350 up and 397 down regulated isoforms by at least two folds over buccal normal in BMC. Study revealed 46 splice variants in normal and 106 in cancer, out of which 10 variants were validated with end point RT-PCR. Expression of two isoforms of CD74 was validated using RT-qPCR and found in accordance with RNA-Seq. Further extensive follow up analysis of modulator genes, isoforms and splice variants found in this study, might be useful in deep understanding of pathological changes in BMC and development of prospective intervention strategies." @default.
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- W2029206815 date "2013-03-01" @default.
- W2029206815 modified "2023-10-17" @default.
- W2029206815 title "RNA-Seq reveals differentially expressed isoforms and novel splice variants in buccal mucosal cancer" @default.
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- W2029206815 doi "https://doi.org/10.1016/j.gene.2012.11.079" @default.
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