FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029209385> ?p ?o ?g. }

• W2029209385 endingPage "550" @default.
• W2029209385 startingPage "537" @default.
• W2029209385 abstract "Our approach to cartilage tissue-engineering scaffolds combines image-based design and solid free-form (SFF) fabrication to create load-bearing constructs with user-defined parameters. In this study, 3-dimensional scaffolds with cubic and ellipsoidal pore architecture were fabricated using poly(propylene fumarate) (PPF). To increase seeding efficiency and cellular retention, hydrogels were used to deliver cells into the scaffolds. The first objective of this study was to evaluate the concentrations of composite hyaluronic acid (HyA) and collagen I hydrogels best able to stimulate proteoglycan synthesis in porcine chondrocytes in vitro and in vivo. The second objective was to evaluate the differences in extracellular matrix production due to pore geometry and scaffold design. For the in vitro assessment, chondrocytes were encapsulated in collagen I hydrogels with varying concentrations of HyA. Hydrogels were cultured for 1 and 2 weeks, and then the sulfated glycosaminoglycan (sGAG) content was quantified using a dimethyl-methylene blue assay. The concentration of HyA best able to increase ECM synthesis was 5% HyA/collagen I, or 0.23 mg/mL HyA. The results from the in vitro experiment were used as culture parameters for the in vivo analysis. Composite 5% HyA/collagen I or collagen I–only hydrogels were used to seed chondrocytes into SFF-fabricated scaffolds made of PPF with designed cubic or ellipsoidal pore geometry. The scaffolds were implanted subcutaneously in immunocompromised mice for 4 weeks. Histomorphometric analyses of sections stained with Safranin O were used to quantify the amount of ECM deposited by cells in the scaffolds. Scaffolds seeded with 5% HyA/collagen hydrogels had significantly greater areas of positive Safranin O staining (approximately 60%, compared with 30% for scaffolds with collagen I hydrogels only), indicating that greater numbers of chondrocytes retained their metabolic activity in the ectopic environment. These scaffolds also had greater stain intensities (corresponding to greater amounts of sGAG in the ECM) than their counterparts seeded with collagen I hydrogels alone. Significant differences in matrix production were not found between the scaffold pore designs. Overall, these results indicate that a combination of composite HyA hydrogels and designed SFF scaffolds could provide a functional tissue-engineered construct for cartilage repair with enhanced tissue regeneration in a load-bearing scaffold." @default.
• W2029209385 created "2016-06-24" @default.
• W2029209385 creator A5004659799 @default.
• W2029209385 creator A5016934014 @default.
• W2029209385 creator A5019484706 @default.
• W2029209385 creator A5053234626 @default.
• W2029209385 date "2007-03-01" @default.
• W2029209385 modified "2023-10-01" @default.
• W2029209385 title "Tissue-Engineered Cartilage Constructs Using Composite Hyaluronic Acid/Collagen I Hydrogels and Designed Poly(Propylene Fumarate) Scaffolds" @default.
• W2029209385 cites W1902549464 @default.
• W2029209385 cites W1972770523 @default.
• W2029209385 cites W1978863827 @default.
• W2029209385 cites W1979313922 @default.
• W2029209385 cites W1983283949 @default.
• W2029209385 cites W2010672204 @default.
• W2029209385 cites W2021531882 @default.
• W2029209385 cites W2031844423 @default.
• W2029209385 cites W2032255522 @default.
• W2029209385 cites W2032883484 @default.
• W2029209385 cites W2035379345 @default.
• W2029209385 cites W2035674015 @default.
• W2029209385 cites W2038494385 @default.
• W2029209385 cites W2042664285 @default.
• W2029209385 cites W2044752140 @default.
• W2029209385 cites W2051994250 @default.
• W2029209385 cites W2061444420 @default.
• W2029209385 cites W2062590686 @default.
• W2029209385 cites W2066928049 @default.
• W2029209385 cites W2067045483 @default.
• W2029209385 cites W2077644606 @default.
• W2029209385 cites W2087635529 @default.
• W2029209385 cites W2088519183 @default.
• W2029209385 cites W2099285233 @default.
• W2029209385 cites W2113042424 @default.
• W2029209385 cites W2113062641 @default.
• W2029209385 cites W2135319676 @default.
• W2029209385 cites W2142720627 @default.
• W2029209385 cites W2170326305 @default.
• W2029209385 cites W2236789517 @default.
• W2029209385 cites W4229982049 @default.
• W2029209385 cites W4240517672 @default.
• W2029209385 doi "https://doi.org/10.1089/ten.2006.0117" @default.
• W2029209385 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17319795" @default.
• W2029209385 hasPublicationYear "2007" @default.
• W2029209385 type Work @default.
• W2029209385 sameAs 2029209385 @default.
• W2029209385 citedByCount "109" @default.
• W2029209385 countsByYear W20292093852012 @default.
• W2029209385 countsByYear W20292093852013 @default.
• W2029209385 countsByYear W20292093852014 @default.
• W2029209385 countsByYear W20292093852015 @default.
• W2029209385 countsByYear W20292093852016 @default.
• W2029209385 countsByYear W20292093852017 @default.
• W2029209385 countsByYear W20292093852018 @default.
• W2029209385 countsByYear W20292093852019 @default.
• W2029209385 countsByYear W20292093852020 @default.
• W2029209385 countsByYear W20292093852021 @default.
• W2029209385 countsByYear W20292093852022 @default.
• W2029209385 countsByYear W20292093852023 @default.
• W2029209385 crossrefType "journal-article" @default.
• W2029209385 hasAuthorship W2029209385A5004659799 @default.
• W2029209385 hasAuthorship W2029209385A5016934014 @default.
• W2029209385 hasAuthorship W2029209385A5019484706 @default.
• W2029209385 hasAuthorship W2029209385A5053234626 @default.
• W2029209385 hasBestOaLocation W20292093852 @default.
• W2029209385 hasConcept C105702510 @default.
• W2029209385 hasConcept C108586683 @default.
• W2029209385 hasConcept C12554922 @default.
• W2029209385 hasConcept C136229726 @default.
• W2029209385 hasConcept C150903083 @default.
• W2029209385 hasConcept C153074725 @default.
• W2029209385 hasConcept C185592680 @default.
• W2029209385 hasConcept C188027245 @default.
• W2029209385 hasConcept C189165786 @default.
• W2029209385 hasConcept C202751555 @default.
• W2029209385 hasConcept C207001950 @default.
• W2029209385 hasConcept C2777529286 @default.
• W2029209385 hasConcept C2780550940 @default.
• W2029209385 hasConcept C49892992 @default.
• W2029209385 hasConcept C55493867 @default.
• W2029209385 hasConcept C71924100 @default.
• W2029209385 hasConcept C86803240 @default.
• W2029209385 hasConcept C89429830 @default.
• W2029209385 hasConceptScore W2029209385C105702510 @default.
• W2029209385 hasConceptScore W2029209385C108586683 @default.
• W2029209385 hasConceptScore W2029209385C12554922 @default.
• W2029209385 hasConceptScore W2029209385C136229726 @default.
• W2029209385 hasConceptScore W2029209385C150903083 @default.
• W2029209385 hasConceptScore W2029209385C153074725 @default.
• W2029209385 hasConceptScore W2029209385C185592680 @default.
• W2029209385 hasConceptScore W2029209385C188027245 @default.
• W2029209385 hasConceptScore W2029209385C189165786 @default.
• W2029209385 hasConceptScore W2029209385C202751555 @default.
• W2029209385 hasConceptScore W2029209385C207001950 @default.
• W2029209385 hasConceptScore W2029209385C2777529286 @default.
• W2029209385 hasConceptScore W2029209385C2780550940 @default.
• W2029209385 hasConceptScore W2029209385C49892992 @default.
• W2029209385 hasConceptScore W2029209385C55493867 @default.

• next