FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029228300> ?p ?o ?g. }

• W2029228300 endingPage "894" @default.
• W2029228300 startingPage "888" @default.
• W2029228300 abstract "The possibility of increasing the efficiency of banana transformation was investigated by particle bombardment of the male flowers of banana plants for constitutive expression of gfp gene. The effects of particle bombardment parameters, such as acceleration pressure, bombardment distance, chamber vacuum pressure, gold microcarrier size, gold quantity, DNA quantity, number of bombardments and pre-culture were examined. Single cauliflower-like bodies (CLBs) clusters, induced from meristemic parts of Musa sapientum cv. Nangka (AAB) male flowers, were bombarded by pCambia1304 plasmid carrying gfp gene driven by the CaMV 35S promoter. Optimal transient expression of green-fluorescent protein (GFP) was obtained when the three-day old cultured tissues were bombarded two times at 1100 psi helium pressure. However, the highest GFP expression was observed when 9 cm was applied as bombardment distance with 28 mmHg chamber vacuum pressure. Gold particle with 1 μm diameter at 60 μg/μL concentrations coated with 1.5 μg/μL of DNA have been used as the optimum bombardment parameter since GFP expression was significantly different compared to other conditions. Application of optimized condition proved effective for the generation of stable transgenic banana plants. PCR and southern blot analyses confirmed the presence and integration of gfp gene in genomic DNA of transformed plants. Transformation frequency achieved with the optimized protocol was 7.5% which was significantly higher than the conventional protocol." @default.
• W2029228300 created "2016-06-24" @default.
• W2029228300 creator A5017350619 @default.
• W2029228300 creator A5020767142 @default.
• W2029228300 creator A5023429386 @default.
• W2029228300 date "2014-07-01" @default.
• W2029228300 modified "2023-10-17" @default.
• W2029228300 title "Optimization of particle bombardment parameters for DNA delivery into the male flowers of banana" @default.
• W2029228300 cites W120788313 @default.
• W2029228300 cites W1990540577 @default.
• W2029228300 cites W1995872125 @default.
• W2029228300 cites W2003553561 @default.
• W2029228300 cites W2006807779 @default.
• W2029228300 cites W2007676372 @default.
• W2029228300 cites W2007723086 @default.
• W2029228300 cites W2018362355 @default.
• W2029228300 cites W2031055871 @default.
• W2029228300 cites W2050770339 @default.
• W2029228300 cites W2051035774 @default.
• W2029228300 cites W2073156154 @default.
• W2029228300 cites W2084229700 @default.
• W2029228300 cites W2084614675 @default.
• W2029228300 cites W2092713296 @default.
• W2029228300 cites W2098043712 @default.
• W2029228300 cites W2125390426 @default.
• W2029228300 cites W2150980388 @default.
• W2029228300 cites W2165491507 @default.
• W2029228300 cites W234997304 @default.
• W2029228300 cites W4251924841 @default.
• W2029228300 cites W4252458518 @default.
• W2029228300 doi "https://doi.org/10.2478/s11756-014-0391-7" @default.
• W2029228300 hasPublicationYear "2014" @default.
• W2029228300 type Work @default.
• W2029228300 sameAs 2029228300 @default.
• W2029228300 citedByCount "4" @default.
• W2029228300 countsByYear W20292283002016 @default.
• W2029228300 countsByYear W20292283002018 @default.
• W2029228300 countsByYear W20292283002023 @default.
• W2029228300 crossrefType "journal-article" @default.
• W2029228300 hasAuthorship W2029228300A5017350619 @default.
• W2029228300 hasAuthorship W2029228300A5020767142 @default.
• W2029228300 hasAuthorship W2029228300A5023429386 @default.
• W2029228300 hasBestOaLocation W20292283001 @default.
• W2029228300 hasConcept C102230213 @default.
• W2029228300 hasConcept C104317684 @default.
• W2029228300 hasConcept C12554922 @default.
• W2029228300 hasConcept C142613039 @default.
• W2029228300 hasConcept C150194340 @default.
• W2029228300 hasConcept C153911025 @default.
• W2029228300 hasConcept C167508917 @default.
• W2029228300 hasConcept C168112101 @default.
• W2029228300 hasConcept C176999471 @default.
• W2029228300 hasConcept C182420341 @default.
• W2029228300 hasConcept C185592680 @default.
• W2029228300 hasConcept C18903297 @default.
• W2029228300 hasConcept C204241405 @default.
• W2029228300 hasConcept C22744801 @default.
• W2029228300 hasConcept C2778517922 @default.
• W2029228300 hasConcept C54355233 @default.
• W2029228300 hasConcept C552990157 @default.
• W2029228300 hasConcept C59822182 @default.
• W2029228300 hasConcept C81885089 @default.
• W2029228300 hasConcept C86803240 @default.
• W2029228300 hasConceptScore W2029228300C102230213 @default.
• W2029228300 hasConceptScore W2029228300C104317684 @default.
• W2029228300 hasConceptScore W2029228300C12554922 @default.
• W2029228300 hasConceptScore W2029228300C142613039 @default.
• W2029228300 hasConceptScore W2029228300C150194340 @default.
• W2029228300 hasConceptScore W2029228300C153911025 @default.
• W2029228300 hasConceptScore W2029228300C167508917 @default.
• W2029228300 hasConceptScore W2029228300C168112101 @default.
• W2029228300 hasConceptScore W2029228300C176999471 @default.
• W2029228300 hasConceptScore W2029228300C182420341 @default.
• W2029228300 hasConceptScore W2029228300C185592680 @default.
• W2029228300 hasConceptScore W2029228300C18903297 @default.
• W2029228300 hasConceptScore W2029228300C204241405 @default.
• W2029228300 hasConceptScore W2029228300C22744801 @default.
• W2029228300 hasConceptScore W2029228300C2778517922 @default.
• W2029228300 hasConceptScore W2029228300C54355233 @default.
• W2029228300 hasConceptScore W2029228300C552990157 @default.
• W2029228300 hasConceptScore W2029228300C59822182 @default.
• W2029228300 hasConceptScore W2029228300C81885089 @default.
• W2029228300 hasConceptScore W2029228300C86803240 @default.
• W2029228300 hasIssue "7" @default.
• W2029228300 hasLocation W20292283001 @default.
• W2029228300 hasOpenAccess W2029228300 @default.
• W2029228300 hasPrimaryLocation W20292283001 @default.
• W2029228300 hasRelatedWork W2010668679 @default.
• W2029228300 hasRelatedWork W2029228300 @default.
• W2029228300 hasRelatedWork W2056947740 @default.
• W2029228300 hasRelatedWork W2086745641 @default.
• W2029228300 hasRelatedWork W2092794690 @default.
• W2029228300 hasRelatedWork W2349059809 @default.
• W2029228300 hasRelatedWork W2380100330 @default.
• W2029228300 hasRelatedWork W2382075157 @default.
• W2029228300 hasRelatedWork W2384870900 @default.
• W2029228300 hasRelatedWork W2942174876 @default.
• W2029228300 hasVolume "69" @default.

• next