Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029263644> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2029263644 endingPage "102" @default.
- W2029263644 startingPage "99" @default.
- W2029263644 abstract "In vitro deletion and transposon mutagenesis experiments were performed to localize the region essential for plasmid RSF0885 replication to a 1.7-kb sequence downstream from the beta-lactamase gene. This locus was named rep, for replication. Plasmid RSF0885 can replicate in both Haemophilus influenzae and Escherichia coli. Replication in E. coli depended on transcription run-off from the beta-lactamase promoter into the rep locus. Insufficient transcription into the rep locus could account for the instability of this plasmid in an E. coli background, a property which reduced its usefulness as a shuttle vector. Therefore, four improved shuttle vectors for H. influenzae and E. coli were constructed. They possess the Co1E1 replication origin for maintenance in E. coli and the plasmid RSF0885 rep locus for maintenance in H. influenzae. Together, they provide twelve unique restriction sites for cloning by insertional inactivation of drug-resistance genes." @default.
- W2029263644 created "2016-06-24" @default.
- W2029263644 creator A5053914570 @default.
- W2029263644 creator A5059615305 @default.
- W2029263644 date "1990-01-01" @default.
- W2029263644 modified "2023-09-25" @default.
- W2029263644 title "Improved plasmid shuttle vectors for Haemophilus influenzae and Escherichia coli" @default.
- W2029263644 cites W1526454910 @default.
- W2029263644 cites W154370734 @default.
- W2029263644 cites W1660950043 @default.
- W2029263644 cites W1945655754 @default.
- W2029263644 cites W1967164846 @default.
- W2029263644 cites W1970486666 @default.
- W2029263644 cites W2016806244 @default.
- W2029263644 cites W2028622989 @default.
- W2029263644 cites W2070159323 @default.
- W2029263644 cites W2127546816 @default.
- W2029263644 cites W2145502740 @default.
- W2029263644 doi "https://doi.org/10.1016/0378-1119(90)90119-c" @default.
- W2029263644 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2179060" @default.
- W2029263644 hasPublicationYear "1990" @default.
- W2029263644 type Work @default.
- W2029263644 sameAs 2029263644 @default.
- W2029263644 citedByCount "6" @default.
- W2029263644 countsByYear W20292636442013 @default.
- W2029263644 crossrefType "journal-article" @default.
- W2029263644 hasAuthorship W2029263644A5053914570 @default.
- W2029263644 hasAuthorship W2029263644A5059615305 @default.
- W2029263644 hasConcept C104317684 @default.
- W2029263644 hasConcept C141231307 @default.
- W2029263644 hasConcept C145640855 @default.
- W2029263644 hasConcept C22744801 @default.
- W2029263644 hasConcept C2776925733 @default.
- W2029263644 hasConcept C28693550 @default.
- W2029263644 hasConcept C40767141 @default.
- W2029263644 hasConcept C4918238 @default.
- W2029263644 hasConcept C523546767 @default.
- W2029263644 hasConcept C54355233 @default.
- W2029263644 hasConcept C547475151 @default.
- W2029263644 hasConcept C58337764 @default.
- W2029263644 hasConcept C80343103 @default.
- W2029263644 hasConcept C84597430 @default.
- W2029263644 hasConcept C86803240 @default.
- W2029263644 hasConcept C92087593 @default.
- W2029263644 hasConceptScore W2029263644C104317684 @default.
- W2029263644 hasConceptScore W2029263644C141231307 @default.
- W2029263644 hasConceptScore W2029263644C145640855 @default.
- W2029263644 hasConceptScore W2029263644C22744801 @default.
- W2029263644 hasConceptScore W2029263644C2776925733 @default.
- W2029263644 hasConceptScore W2029263644C28693550 @default.
- W2029263644 hasConceptScore W2029263644C40767141 @default.
- W2029263644 hasConceptScore W2029263644C4918238 @default.
- W2029263644 hasConceptScore W2029263644C523546767 @default.
- W2029263644 hasConceptScore W2029263644C54355233 @default.
- W2029263644 hasConceptScore W2029263644C547475151 @default.
- W2029263644 hasConceptScore W2029263644C58337764 @default.
- W2029263644 hasConceptScore W2029263644C80343103 @default.
- W2029263644 hasConceptScore W2029263644C84597430 @default.
- W2029263644 hasConceptScore W2029263644C86803240 @default.
- W2029263644 hasConceptScore W2029263644C92087593 @default.
- W2029263644 hasIssue "1" @default.
- W2029263644 hasLocation W20292636441 @default.
- W2029263644 hasLocation W20292636442 @default.
- W2029263644 hasOpenAccess W2029263644 @default.
- W2029263644 hasPrimaryLocation W20292636441 @default.
- W2029263644 hasRelatedWork W1546809062 @default.
- W2029263644 hasRelatedWork W1660950043 @default.
- W2029263644 hasRelatedWork W2017504871 @default.
- W2029263644 hasRelatedWork W2029263644 @default.
- W2029263644 hasRelatedWork W2048173983 @default.
- W2029263644 hasRelatedWork W2080138031 @default.
- W2029263644 hasRelatedWork W2101509711 @default.
- W2029263644 hasRelatedWork W2153512555 @default.
- W2029263644 hasRelatedWork W2524850911 @default.
- W2029263644 hasRelatedWork W4220995072 @default.
- W2029263644 hasVolume "86" @default.
- W2029263644 isParatext "false" @default.
- W2029263644 isRetracted "false" @default.
- W2029263644 magId "2029263644" @default.
- W2029263644 workType "article" @default.