FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029276564> ?p ?o ?g. }

• W2029276564 endingPage "2947" @default.
• W2029276564 startingPage "2937" @default.
• W2029276564 abstract "Study of the in vivo functions of the insulin-like growth factor binding proteins (IGFBPs) is complicated by their variety (six molecular species) and the differences in their expression related to tissue of origin and stage of development. To investigate the physiological role of IGFBP-1 in the bloodstream, we induced hepatic overexpression of IGFBP-1 in transgenic mice, placing human IGFBP-1 (hIGFBP-1) cDNA under the control of the alpha1-antitrypsin promoter so as to obtain liver-specific expression. Five transgenic founder mice were raised, only two of which (lines 124 and 149) produced transgenic offspring. Northern blotting revealed transgene expression exclusively in the liver during fetal life and unchanged through to adulthood, whereas expression of the endogenous gene was undetectable beyond 10-15 days postnatally. hIGFBP-1 was detected by western immunoblotting in the plasma of transgenic mice and IRMAs yielded mean concentrations of 2.41 +/- 0.33 ng/ml and 13.69 +/- 1.42 ng/ml in homozygous animals of lines 124 and 149, respectively. In the latter, IGFBP-1 levels were distinctly higher than in heterozygotes (2.99 +/- 0.39 ng/ml), P < 0.0001. These levels remained stable in each given animal and did not change with age. Plasma concentrations of IGF-I measured in line 149 exhibited the well-known profile of an increase from birth up to puberty. Values for heterozygotes were similar to those for wild-type mice, with adult levels (544 +/- 98 ng/ml) slightly below those of controls (630 +/- 56 ng/ml), P < 0.05. In homozygotes they were distinctly lower, with adult levels of 370 +/- 75 ng/ml, P = 0.001. In heterozygous and homozygous adults, there was a negative correlation between IGF-I and IGFBP-1 concentrations (r = 0.8, P < 0.0001), suggesting a link between transgene expression and IGF-I levels. Study of body weight gain in line 149 revealed growth retardation within the first weeks after birth, which was marked in homozygous males and females (P < 0.001) but also present in heterozygous males (P = 0.002), indicating some relationship with transgene expression. In addition, body weight in adult mice was negatively correlated to plasma concentrations of IGFBP-1 (r = 0.7, P < 0.0001). Reproductive function also appeared to be severely affected, especially in homozygous females: mating that failed to result in pregnancy in half of the homozygous females crossed with nontransgenic males, suggestive of impaired fertilization or implantation; interrupted or prolonged pregnancies with fetal and neonatal death. Litter size was reduced in transgenic females (by about half in homozygotes) and in nontransgenic females mated with homozygous males, resulting from pre- or neonatal mortality. Moreover, deaths occurred within the first 5 days of life, with an incidence of approximately 50% in the litters of homozygous females, 12-18% among heterozygotes mated with nontransgenic or heterozygous males, respectively, and 30% among those mated with homozygous males. These results, suggesting that fetal transgene expression largely accounted for ante- and perinatal mortality, were confirmed by the predominance of homozygotes among those that could be analyzed genetically. Similarly impaired reproductive function was seen in line 124, but to a lesser degree. Although the mechanisms responsible for these disorders remain to be determined, our results indicate that permanent and uncontrolled hepatic expression of IGFBP-1, even at low levels, affects fertility in females and both ante- and postnatal development." @default.
• W2029276564 created "2016-06-24" @default.
• W2029276564 creator A5012052565 @default.
• W2029276564 creator A5013993277 @default.
• W2029276564 creator A5020315292 @default.
• W2029276564 creator A5078149259 @default.
• W2029276564 creator A5081250380 @default.
• W2029276564 date "1997-07-01" @default.
• W2029276564 modified "2023-10-18" @default.
• W2029276564 title "Liver-Specific Expression of Human Insulin-Like Growth Factor Binding Protein-1 in Transgenic Mice: Repercussions on Reproduction, Ante- and Perinatal Mortality and Postnatal Growth¹" @default.
• W2029276564 cites W1484163259 @default.
• W2029276564 cites W1879994568 @default.
• W2029276564 cites W1972083618 @default.
• W2029276564 cites W1972308754 @default.
• W2029276564 cites W1972819401 @default.
• W2029276564 cites W1975174882 @default.
• W2029276564 cites W1976125993 @default.
• W2029276564 cites W1980701912 @default.
• W2029276564 cites W1987709138 @default.
• W2029276564 cites W1993672117 @default.
• W2029276564 cites W1994780917 @default.
• W2029276564 cites W2005438264 @default.
• W2029276564 cites W2008122244 @default.
• W2029276564 cites W2011861484 @default.
• W2029276564 cites W2013271253 @default.
• W2029276564 cites W2015843482 @default.
• W2029276564 cites W2016562893 @default.
• W2029276564 cites W2017949036 @default.
• W2029276564 cites W2034816153 @default.
• W2029276564 cites W2038555949 @default.
• W2029276564 cites W2039825798 @default.
• W2029276564 cites W2046833627 @default.
• W2029276564 cites W2050834070 @default.
• W2029276564 cites W2057155280 @default.
• W2029276564 cites W2063450941 @default.
• W2029276564 cites W2063840332 @default.
• W2029276564 cites W2072514327 @default.
• W2029276564 cites W2080988561 @default.
• W2029276564 cites W2082874604 @default.
• W2029276564 cites W2094207204 @default.
• W2029276564 cites W2094626343 @default.
• W2029276564 cites W2127006469 @default.
• W2029276564 cites W2134812217 @default.
• W2029276564 doi "https://doi.org/10.1210/endo.138.7.5282" @default.
• W2029276564 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9202238" @default.
• W2029276564 hasPublicationYear "1997" @default.
• W2029276564 type Work @default.
• W2029276564 sameAs 2029276564 @default.
• W2029276564 citedByCount "100" @default.
• W2029276564 countsByYear W20292765642012 @default.
• W2029276564 countsByYear W20292765642014 @default.
• W2029276564 countsByYear W20292765642015 @default.
• W2029276564 countsByYear W20292765642016 @default.
• W2029276564 countsByYear W20292765642020 @default.
• W2029276564 countsByYear W20292765642021 @default.
• W2029276564 countsByYear W20292765642022 @default.
• W2029276564 crossrefType "journal-article" @default.
• W2029276564 hasAuthorship W2029276564A5012052565 @default.
• W2029276564 hasAuthorship W2029276564A5013993277 @default.
• W2029276564 hasAuthorship W2029276564A5020315292 @default.
• W2029276564 hasAuthorship W2029276564A5078149259 @default.
• W2029276564 hasAuthorship W2029276564A5081250380 @default.
• W2029276564 hasBestOaLocation W20292765641 @default.
• W2029276564 hasConcept C102230213 @default.
• W2029276564 hasConcept C104317684 @default.
• W2029276564 hasConcept C112672928 @default.
• W2029276564 hasConcept C126322002 @default.
• W2029276564 hasConcept C134018914 @default.
• W2029276564 hasConcept C135763542 @default.
• W2029276564 hasConcept C141035611 @default.
• W2029276564 hasConcept C150194340 @default.
• W2029276564 hasConcept C153471976 @default.
• W2029276564 hasConcept C170493617 @default.
• W2029276564 hasConcept C172680121 @default.
• W2029276564 hasConcept C207001950 @default.
• W2029276564 hasConcept C2775960820 @default.
• W2029276564 hasConcept C2779234561 @default.
• W2029276564 hasConcept C2780378035 @default.
• W2029276564 hasConcept C2780689927 @default.
• W2029276564 hasConcept C54355233 @default.
• W2029276564 hasConcept C71924100 @default.
• W2029276564 hasConcept C85265743 @default.
• W2029276564 hasConcept C86803240 @default.
• W2029276564 hasConceptScore W2029276564C102230213 @default.
• W2029276564 hasConceptScore W2029276564C104317684 @default.
• W2029276564 hasConceptScore W2029276564C112672928 @default.
• W2029276564 hasConceptScore W2029276564C126322002 @default.
• W2029276564 hasConceptScore W2029276564C134018914 @default.
• W2029276564 hasConceptScore W2029276564C135763542 @default.
• W2029276564 hasConceptScore W2029276564C141035611 @default.
• W2029276564 hasConceptScore W2029276564C150194340 @default.
• W2029276564 hasConceptScore W2029276564C153471976 @default.
• W2029276564 hasConceptScore W2029276564C170493617 @default.
• W2029276564 hasConceptScore W2029276564C172680121 @default.
• W2029276564 hasConceptScore W2029276564C207001950 @default.
• W2029276564 hasConceptScore W2029276564C2775960820 @default.
• W2029276564 hasConceptScore W2029276564C2779234561 @default.
• W2029276564 hasConceptScore W2029276564C2780378035 @default.

• next