FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029290423> ?p ?o ?g. }

• W2029290423 endingPage "8862" @default.
• W2029290423 startingPage "8849" @default.
• W2029290423 abstract "Highly conserved molecular chaperone Hsp70 heat shock proteins play a key role in maintaining protein homeostasis (proteostasis). DnaK, a major Hsp70 in Escherichia coli, has been widely used as a paradigm for studying Hsp70s. In the absence of ATP, purified DnaK forms low-ordered oligomer, whereas ATP binding shifts the equilibrium toward the monomer. Recently, we solved the crystal structure of DnaK in complex with ATP. There are two molecules of DnaK-ATP in the asymmetric unit. Interestingly, the interfaces between the two molecules of DnaK are large with good surface complementarity, suggesting functional importance of this crystallographic dimer. Biochemical analyses of DnaK protein supported the formation of dimer in solution. Furthermore, our cross-linking experiment based on the DnaK-ATP structure confirmed that DnaK forms specific dimer in an ATP-dependent manner. To understand the physiological function of the dimer, we mutated five residues on the dimer interface. Four mutations, R56A, T301A, N537A, and D540A, resulted in loss of chaperone activity and compromised the formation of dimer, indicating the functional importance of the dimer. Surprisingly, neither the intrinsic biochemical activities, the ATP-induced allosteric coupling, nor GrpE co-chaperone interaction is affected appreciably in all of the mutations except for R56A. Unexpectedly, the interaction with co-chaperone Hsp40 is significantly compromised. In summary, this study suggests that DnaK forms a transient dimer upon ATP binding, and this dimer is essential for the efficient interaction of DnaK with Hsp40." @default.
• W2029290423 created "2016-06-24" @default.
• W2029290423 creator A5003413476 @default.
• W2029290423 creator A5008792115 @default.
• W2029290423 creator A5009049832 @default.
• W2029290423 creator A5015005766 @default.
• W2029290423 creator A5019693747 @default.
• W2029290423 creator A5055347404 @default.
• W2029290423 creator A5057249150 @default.
• W2029290423 date "2015-04-01" @default.
• W2029290423 modified "2023-10-18" @default.
• W2029290423 title "A Functional DnaK Dimer Is Essential for the Efficient Interaction with Hsp40 Heat Shock Protein" @default.
• W2029290423 cites W1511249469 @default.
• W2029290423 cites W1541948779 @default.
• W2029290423 cites W1567164927 @default.
• W2029290423 cites W1879327185 @default.
• W2029290423 cites W1963770237 @default.
• W2029290423 cites W1963807823 @default.
• W2029290423 cites W1964995688 @default.
• W2029290423 cites W1966923623 @default.
• W2029290423 cites W1967522935 @default.
• W2029290423 cites W1967960279 @default.
• W2029290423 cites W1972110853 @default.
• W2029290423 cites W1972121275 @default.
• W2029290423 cites W1972770744 @default.
• W2029290423 cites W1977385837 @default.
• W2029290423 cites W1978014671 @default.
• W2029290423 cites W1978203600 @default.
• W2029290423 cites W1982991372 @default.
• W2029290423 cites W1983544779 @default.
• W2029290423 cites W1985650639 @default.
• W2029290423 cites W1996623614 @default.
• W2029290423 cites W1996756446 @default.
• W2029290423 cites W1997718936 @default.
• W2029290423 cites W1999815303 @default.
• W2029290423 cites W1999819298 @default.
• W2029290423 cites W2002074105 @default.
• W2029290423 cites W2014294043 @default.
• W2029290423 cites W2016396087 @default.
• W2029290423 cites W2028705478 @default.
• W2029290423 cites W2034943952 @default.
• W2029290423 cites W2039975390 @default.
• W2029290423 cites W2043693325 @default.
• W2029290423 cites W2045872211 @default.
• W2029290423 cites W2047270263 @default.
• W2029290423 cites W2047477750 @default.
• W2029290423 cites W2051563344 @default.
• W2029290423 cites W2051592058 @default.
• W2029290423 cites W2052437470 @default.
• W2029290423 cites W2055269320 @default.
• W2029290423 cites W2060303895 @default.
• W2029290423 cites W2060864422 @default.
• W2029290423 cites W2062784876 @default.
• W2029290423 cites W2063502932 @default.
• W2029290423 cites W2064123540 @default.
• W2029290423 cites W2064292796 @default.
• W2029290423 cites W2064676619 @default.
• W2029290423 cites W2064878094 @default.
• W2029290423 cites W2067128865 @default.
• W2029290423 cites W2069911394 @default.
• W2029290423 cites W2073920150 @default.
• W2029290423 cites W2076159058 @default.
• W2029290423 cites W2076945013 @default.
• W2029290423 cites W2078010704 @default.
• W2029290423 cites W2085703132 @default.
• W2029290423 cites W2087689873 @default.
• W2029290423 cites W2088305244 @default.
• W2029290423 cites W2089457735 @default.
• W2029290423 cites W2092180405 @default.
• W2029290423 cites W2092198909 @default.
• W2029290423 cites W2093983285 @default.
• W2029290423 cites W2095231417 @default.
• W2029290423 cites W2096966529 @default.
• W2029290423 cites W2107334607 @default.
• W2029290423 cites W2109936231 @default.
• W2029290423 cites W2120396047 @default.
• W2029290423 cites W2146143166 @default.
• W2029290423 cites W2154290102 @default.
• W2029290423 cites W2156447960 @default.
• W2029290423 cites W2157369544 @default.
• W2029290423 cites W2161577974 @default.
• W2029290423 cites W2164018178 @default.
• W2029290423 cites W2204934950 @default.
• W2029290423 doi "https://doi.org/10.1074/jbc.m114.596288" @default.
• W2029290423 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4423677" @default.
• W2029290423 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25635056" @default.
• W2029290423 hasPublicationYear "2015" @default.
• W2029290423 type Work @default.
• W2029290423 sameAs 2029290423 @default.
• W2029290423 citedByCount "61" @default.
• W2029290423 countsByYear W20292904232015 @default.
• W2029290423 countsByYear W20292904232016 @default.
• W2029290423 countsByYear W20292904232017 @default.
• W2029290423 countsByYear W20292904232018 @default.
• W2029290423 countsByYear W20292904232019 @default.
• W2029290423 countsByYear W20292904232020 @default.
• W2029290423 countsByYear W20292904232021 @default.
• W2029290423 countsByYear W20292904232022 @default.

• next