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• W2029333246 abstract "ObjectiveTo investigate the anti-inflammatory effects of Sirt1 and its roles in steroidogenesis in Leydig cells.DesignIn vitro cell culture study.SettingReproductive medical center.Animal(s)C57BL/6 male mice.Intervention(s)TM3 Leydig cells were treated with proinflammatory cytokines including tumor necrosis factor-α, interleukin-6, or interleukin-1β.Main Outcomes Measure(s)Sirt1 mRNA and protein levels were measured by real-time polymerase chain reaction and Western blotting in TM3 Leydig cells treated with proinflammatory cytokines. The cell viability was determined using MTT and BrdU incorporation assays. The effect of Sirt1 on the coactivation of steroidogenic factor 1 (SF-1) was characterized by coimmunoprecipitation and luciferase reporter assays.Result(s)Sirt1 mRNA and protein levels were significantly down-regulated by proinflammatory cytokines treatment in TM3 Leydig cells. Sirt1 agonist or overexpression could efficiently protect cytotoxicity induced by proinflammatory cytokines. Sirt1 promoted steroidogenesis through its coactivation of SF-1.Conclusion(s)Sirt1 plays protective roles and promotes steroidogenesis in Leydig cells through its anti-inflammatory actions and coactivation of SF-1. To investigate the anti-inflammatory effects of Sirt1 and its roles in steroidogenesis in Leydig cells. In vitro cell culture study. Reproductive medical center. C57BL/6 male mice. TM3 Leydig cells were treated with proinflammatory cytokines including tumor necrosis factor-α, interleukin-6, or interleukin-1β. Sirt1 mRNA and protein levels were measured by real-time polymerase chain reaction and Western blotting in TM3 Leydig cells treated with proinflammatory cytokines. The cell viability was determined using MTT and BrdU incorporation assays. The effect of Sirt1 on the coactivation of steroidogenic factor 1 (SF-1) was characterized by coimmunoprecipitation and luciferase reporter assays. Sirt1 mRNA and protein levels were significantly down-regulated by proinflammatory cytokines treatment in TM3 Leydig cells. Sirt1 agonist or overexpression could efficiently protect cytotoxicity induced by proinflammatory cytokines. Sirt1 promoted steroidogenesis through its coactivation of SF-1. Sirt1 plays protective roles and promotes steroidogenesis in Leydig cells through its anti-inflammatory actions and coactivation of SF-1." @default.
• W2029333246 created "2016-06-24" @default.
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• W2029333246 date "2012-07-01" @default.
• W2029333246 modified "2023-10-14" @default.
• W2029333246 title "Sirt1 exerts anti-inflammatory effects and promotes steroidogenesis in Leydig cells" @default.
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• W2029333246 doi "https://doi.org/10.1016/j.fertnstert.2012.04.008" @default.
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