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- W2029358384 abstract "We have used optical tweezers to analyse the effect of Blebbistatin and Cytochalasin D on the force exerted by lamellipodia from developing growth cones of isolated Dorsal Root Ganglia neurons. In the presence of 20 μM Blebbistatin, a well known inhibitor of Myosin II, force generation still occurs but with a slower rate and a reduced velocity of the lamellipodium leading edge. Frequency of elementary jumps underlying force generation was reduced by approximately 50%. The distribution of amplitudes of elementary jumps was fitted with the exponential distribution, A+ e - (j+/j+∗) and A- e - (j-/j-∗), with the values of 3.4±0.9 and 3.2±0.8 nm for the mean size of positive j+∗ and negative j-∗ jumps in the presence of Blebbistatin and 5.2±1.3 and 4.9±1.2 nm in control conditions.The addition of 12.5-50 nM Cytochalasin D, an inhibitor of F-actin polymerization slowed down lamellipodia motion but did not abolish force generation. In the presence of 50 nM Cytochalasin D, growth cones moved very slowly, with a velocity two times less than in control conditions. Small concentrations of both drugs significantly decreased the occurrence of shovel like events, during which the lamellipodium had waves of protrusion/retraction and lifted up by 1-3 microns. Therefore, shovel like events could be mediated by specific molecular mechanisms, different from those underlying lateral protrusions and retractions." @default.
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- W2029358384 date "2012-01-01" @default.
- W2029358384 modified "2023-10-01" @default.
- W2029358384 title "The Effect of Blebbistatin and Cytochalasin D on Force Generation in DRG Lamellipodia" @default.
- W2029358384 doi "https://doi.org/10.1016/j.bpj.2011.11.1916" @default.
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