Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029360315> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2029360315 endingPage "308" @default.
- W2029360315 startingPage "299" @default.
- W2029360315 abstract "An enzyme-linked immunosorbent assay (ELISA)-elution assay was developed to screen a large variety of elution buffers for selection of a suitable one for purification of the fusion protein FV/TNF-α by affinity chromatography. Various commonly used buffer systems utilizing widely differing conditions such as extreme pH, denaturants, chaotropic ions and polarity reducing reagents were investigated. Ammonia solution (1 M, pH 11.5) proved to exert the most suitable influence on dissociation of the FV/TNF-α/TAG72 complex while having a minimal protein denaturing effect on FV/TNF-α. The total yield of purified FV/TNF-α using the TAG72-affinity column with this elution system was 300-fold higher than that using the common elution buffer, 0.1 M glycine, 0.5 M NaCl, pH 2.7. Our study indicates that the ELISA-elution assay will be most useful in the selection of suitable elution buffers for affinity chromatography." @default.
- W2029360315 created "2016-06-24" @default.
- W2029360315 creator A5014500064 @default.
- W2029360315 creator A5016826042 @default.
- W2029360315 creator A5071965865 @default.
- W2029360315 date "1999-08-01" @default.
- W2029360315 modified "2023-09-30" @default.
- W2029360315 title "Enzyme-linked immunosorbent assay-based selection and optimization of elution buffer for TAG72-affinity chromatography" @default.
- W2029360315 cites W1551834526 @default.
- W2029360315 cites W1974977468 @default.
- W2029360315 cites W1978087981 @default.
- W2029360315 cites W1991013932 @default.
- W2029360315 cites W2005153139 @default.
- W2029360315 cites W2005600350 @default.
- W2029360315 cites W2016407884 @default.
- W2029360315 cites W2019190216 @default.
- W2029360315 cites W2032060707 @default.
- W2029360315 cites W2072030247 @default.
- W2029360315 cites W2073994030 @default.
- W2029360315 cites W2101022027 @default.
- W2029360315 cites W2118032120 @default.
- W2029360315 cites W280875868 @default.
- W2029360315 cites W965782870 @default.
- W2029360315 doi "https://doi.org/10.1016/s0378-4347(99)00250-9" @default.
- W2029360315 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10510784" @default.
- W2029360315 hasPublicationYear "1999" @default.
- W2029360315 type Work @default.
- W2029360315 sameAs 2029360315 @default.
- W2029360315 citedByCount "4" @default.
- W2029360315 crossrefType "journal-article" @default.
- W2029360315 hasAuthorship W2029360315A5014500064 @default.
- W2029360315 hasAuthorship W2029360315A5016826042 @default.
- W2029360315 hasAuthorship W2029360315A5071965865 @default.
- W2029360315 hasConcept C102362939 @default.
- W2029360315 hasConcept C147789679 @default.
- W2029360315 hasConcept C179247698 @default.
- W2029360315 hasConcept C181199279 @default.
- W2029360315 hasConcept C185592680 @default.
- W2029360315 hasConcept C206967267 @default.
- W2029360315 hasConcept C40875361 @default.
- W2029360315 hasConcept C43617362 @default.
- W2029360315 hasConcept C55493867 @default.
- W2029360315 hasConcept C73826308 @default.
- W2029360315 hasConceptScore W2029360315C102362939 @default.
- W2029360315 hasConceptScore W2029360315C147789679 @default.
- W2029360315 hasConceptScore W2029360315C179247698 @default.
- W2029360315 hasConceptScore W2029360315C181199279 @default.
- W2029360315 hasConceptScore W2029360315C185592680 @default.
- W2029360315 hasConceptScore W2029360315C206967267 @default.
- W2029360315 hasConceptScore W2029360315C40875361 @default.
- W2029360315 hasConceptScore W2029360315C43617362 @default.
- W2029360315 hasConceptScore W2029360315C55493867 @default.
- W2029360315 hasConceptScore W2029360315C73826308 @default.
- W2029360315 hasIssue "2" @default.
- W2029360315 hasLocation W20293603151 @default.
- W2029360315 hasLocation W20293603152 @default.
- W2029360315 hasOpenAccess W2029360315 @default.
- W2029360315 hasPrimaryLocation W20293603151 @default.
- W2029360315 hasRelatedWork W1894953813 @default.
- W2029360315 hasRelatedWork W1983211232 @default.
- W2029360315 hasRelatedWork W2012215896 @default.
- W2029360315 hasRelatedWork W2027971524 @default.
- W2029360315 hasRelatedWork W2029360315 @default.
- W2029360315 hasRelatedWork W2056193646 @default.
- W2029360315 hasRelatedWork W2095542641 @default.
- W2029360315 hasRelatedWork W2154123486 @default.
- W2029360315 hasRelatedWork W2185419044 @default.
- W2029360315 hasRelatedWork W4234604621 @default.
- W2029360315 hasVolume "731" @default.
- W2029360315 isParatext "false" @default.
- W2029360315 isRetracted "false" @default.
- W2029360315 magId "2029360315" @default.
- W2029360315 workType "article" @default.