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• W2029362004 abstract "Voltage‐dependent Ca 2+ channels were identified in CHO‐K1 cells, currently used in molecular biology studies. Experimental data obtained at both macroscopic and single‐channel levels using the patch‐clamp technique show that the Ca 2+ current in CHO cells is similar to the high‐threshold L‐type of Ca 2+ current previously observed in excitable cells. It can be carried by Ca 2+ or Ba 2+ ions, blocked by both inorganic (Co 2+ ) and organic (nifedipine, isradipine) Ca 2+ channel blockers. The unitary Ca 2+ channel activity was characterized by a conductance of 19 pS in 60 mM Ca 2+ , by single exponential distribution of open times ( t = 0.84 ms) and biexponential distribution of closed times ( t f , = 1.67 ms, t s = 7.9 ms ). However, the functional role of these Ca 2+ channels in CHO cells remains unclear." @default.
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• W2029362004 date "1994-08-01" @default.
• W2029362004 modified "2023-09-30" @default.
• W2029362004 title "Voltage-dependent Ca²⁺channels in Chinese hamster ovary (CHO) cells" @default.
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• W2029362004 doi "https://doi.org/10.1016/0014-5793(94)00690-3" @default.
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