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• W2029366643 endingPage "2711" @default.
• W2029366643 startingPage "2703" @default.
• W2029366643 abstract "Objective To evaluate the role of the extracellular inorganic pyrophosphate (ePPi)–generating ectoenzyme cartilage intermediate layer protein/nucleotide pyrophosphohydrolase (CILP/NTPPH) in chondrocyte PPi elaboration, we studied CILP/NTPPH expression in response to growth factors during aging. Methods Porcine chondrocytes from adult (3–4-year-old) and young (2-week-old) animals were stimulated with transforming growth factor β1 (TGFβ1), which enhances ePPi elaboration, and/or insulin-like growth factor 1 (IGF-1), which diminishes ePPi elaboration. Measurements of ePPi, NTPPH enzyme activity, Western blot analysis, reverse transcriptase–polymerase chain reaction (RT-PCR), and Northern blot analysis were performed. Results Elaboration of ePPi into conditioned media from adult chondrocytes was significantly increased by TGFβ1 and significantly inhibited by IGF-1, but no significant differences were observed in young chondrocytes. The protein levels of CILP/NTPPH by Western analysis in the media from adult and young porcine chondrocytes were increased by TGFβ1. RT-PCR and Northern analysis showed that CILP/NTPPH messenger RNA (mRNA) expression in both adult and young chondrocytes was increased by TGFβ1 and decreased by IGF-1, but these changes were less significant in the young chondrocytes. Basal and TGFβ1–up-regulated levels of CILP/NTPPH expression were higher in adult chondrocytes than in young chondrocytes. Conclusion These results provide evidence that CILP/NTPPH expression and ePPi elaboration are concomitantly stimulated by TGFβ1 and down-regulated by IGF-1, especially in adult chondrocytes, implicating CILP/NTPPH as a functional participant in ePPi elaboration. Increased CILP/NTPPH mRNA expression in chondrocytes derived from aged animals compared with young animals might promote the formation of calcium pyrophosphate dihydrate crystals in aged cartilage." @default.
• W2029366643 created "2016-06-24" @default.
• W2029366643 creator A5010021838 @default.
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• W2029366643 date "2000-12-01" @default.
• W2029366643 modified "2023-09-26" @default.
• W2029366643 title "Expression of cartilage intermediate layer protein/nucleotide pyrophosphohydrolase parallels the production of extracellular inorganic pyrophosphate in response to growth factors and with aging" @default.
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• W2029366643 doi "https://doi.org/10.1002/1529-0131(200012)43:12<2703::aid-anr10>3.0.co;2-y" @default.
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