FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029371986> ?p ?o ?g. }

• W2029371986 endingPage "774" @default.
• W2029371986 startingPage "761" @default.
• W2029371986 abstract "AMP-activated protein kinase (AMPK) has recently been implicated in the control of preproinsulin gene expression in pancreatic islet β-cells [da Silva Xavier, Leclerc, Salt, Doiron, Hardie, Kahn and Rutter (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 4023–4028]. Using pharmacological and molecular strategies to regulate AMPK activity in rat islets and clonal MIN6 β-cells, we show here that the effects of AMPK are exerted largely upstream of insulin release. Thus forced increases in AMPK activity achieved pharmacologically with 5-amino-4-imidazolecarboxamide riboside (AICAR), or by adenoviral overexpression of a truncated, constitutively active form of the enzyme (AMPKα1.T172D), blocked glucose-stimulated insulin secretion. In MIN6 cells, activation of AMPK suppressed glucose metabolism, as assessed by changes in total, cytosolic or mitochondrial [ATP] and NAD(P)H, and reduced increases in intracellular [Ca2+] caused by either glucose or tolbutamide. By contrast, inactivation of AMPK by expression of a dominant-negative form of the enzyme mutated in the catalytic site (AMPKα1.D157A) did not affect glucose-stimulated increases in [ATP], NAD(P)H or intracellular [Ca2+], but led to the unregulated release of insulin. These results indicate that inhibition of AMPK by glucose is essential for the activation of insulin secretion by the sugar, and may contribute to the transcriptional stimulation of the preproinsulin gene. Modulation of AMPK activity in the β-cell may thus represent a novel therapeutic strategy for the treatment of type 2 diabetes mellitus." @default.
• W2029371986 created "2016-06-24" @default.
• W2029371986 creator A5001913005 @default.
• W2029371986 creator A5015737554 @default.
• W2029371986 creator A5020099244 @default.
• W2029371986 creator A5080968511 @default.
• W2029371986 creator A5087148765 @default.
• W2029371986 creator A5089992606 @default.
• W2029371986 date "2003-05-01" @default.
• W2029371986 modified "2023-09-23" @default.
• W2029371986 title "Role for AMP-activated protein kinase in glucose-stimulated insulin secretion and preproinsulin gene expression" @default.
• W2029371986 cites W103234022 @default.
• W2029371986 cites W1498789021 @default.
• W2029371986 cites W1529396499 @default.
• W2029371986 cites W1545683217 @default.
• W2029371986 cites W1565979608 @default.
• W2029371986 cites W1570630058 @default.
• W2029371986 cites W1581906851 @default.
• W2029371986 cites W168671060 @default.
• W2029371986 cites W1788294099 @default.
• W2029371986 cites W1901140894 @default.
• W2029371986 cites W1913661755 @default.
• W2029371986 cites W1968789295 @default.
• W2029371986 cites W1968900685 @default.
• W2029371986 cites W1969914617 @default.
• W2029371986 cites W1972762474 @default.
• W2029371986 cites W1976261899 @default.
• W2029371986 cites W1981668713 @default.
• W2029371986 cites W1983844571 @default.
• W2029371986 cites W1987787422 @default.
• W2029371986 cites W1997010909 @default.
• W2029371986 cites W2000659699 @default.
• W2029371986 cites W2000795392 @default.
• W2029371986 cites W2006707086 @default.
• W2029371986 cites W2012898950 @default.
• W2029371986 cites W2016944998 @default.
• W2029371986 cites W2020882452 @default.
• W2029371986 cites W2027411301 @default.
• W2029371986 cites W2028754125 @default.
• W2029371986 cites W2029121966 @default.
• W2029371986 cites W2033823674 @default.
• W2029371986 cites W2035748369 @default.
• W2029371986 cites W2036784822 @default.
• W2029371986 cites W2036848107 @default.
• W2029371986 cites W2037501075 @default.
• W2029371986 cites W2040015414 @default.
• W2029371986 cites W2049787853 @default.
• W2029371986 cites W2051770908 @default.
• W2029371986 cites W2063564339 @default.
• W2029371986 cites W2065900499 @default.
• W2029371986 cites W2067511291 @default.
• W2029371986 cites W2068245283 @default.
• W2029371986 cites W2069978422 @default.
• W2029371986 cites W2076927702 @default.
• W2029371986 cites W2080730085 @default.
• W2029371986 cites W2082903399 @default.
• W2029371986 cites W2084918846 @default.
• W2029371986 cites W2087136121 @default.
• W2029371986 cites W2092162739 @default.
• W2029371986 cites W2096979086 @default.
• W2029371986 cites W2097198889 @default.
• W2029371986 cites W2105072053 @default.
• W2029371986 cites W2106472841 @default.
• W2029371986 cites W2114339108 @default.
• W2029371986 cites W2118969904 @default.
• W2029371986 cites W2126100001 @default.
• W2029371986 cites W2127109293 @default.
• W2029371986 cites W2127576047 @default.
• W2029371986 cites W2134535786 @default.
• W2029371986 cites W2138226825 @default.
• W2029371986 cites W2140192657 @default.
• W2029371986 cites W2141063928 @default.
• W2029371986 cites W2141260882 @default.
• W2029371986 cites W2147594695 @default.
• W2029371986 cites W2148694780 @default.
• W2029371986 cites W2156565020 @default.
• W2029371986 cites W2160905895 @default.
• W2029371986 cites W2162324890 @default.
• W2029371986 cites W2165577641 @default.
• W2029371986 cites W2167930052 @default.
• W2029371986 cites W2170880090 @default.
• W2029371986 cites W2199557803 @default.
• W2029371986 cites W2284365450 @default.
• W2029371986 cites W2316673780 @default.
• W2029371986 cites W2334442790 @default.
• W2029371986 cites W318583751 @default.
• W2029371986 cites W3889518 @default.
• W2029371986 cites W817448673 @default.
• W2029371986 doi "https://doi.org/10.1042/bj20021812" @default.
• W2029371986 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1223356" @default.
• W2029371986 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12589707" @default.
• W2029371986 hasPublicationYear "2003" @default.
• W2029371986 type Work @default.
• W2029371986 sameAs 2029371986 @default.
• W2029371986 citedByCount "250" @default.
• W2029371986 countsByYear W20293719862012 @default.
• W2029371986 countsByYear W20293719862013 @default.
• W2029371986 countsByYear W20293719862014 @default.

• next