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- W2029402925 abstract "Hydroxyurea inhibition is a convenient method of suppressing replicative DNA synthesis for DNA excision-repair measurement by the BND cellulose technique. Nonetheless, hydroxyurea can introduce artefacts by direct reaction with repair-inducing compounds and by long-term inhibition of the overall repair process. A simple technique of overcoming these problems is described. Cells are reacted with repair-inducing compounds in the absence of hydroxyurea, the cells are washed free of inducer, hydroxyurea is added to 2 mM, and after a short period to establish replication inhibition, 3H dThd is added and repair measured over a one-hour incubation period." @default.
- W2029402925 created "2016-06-24" @default.
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- W2029402925 date "1980-01-01" @default.
- W2029402925 modified "2023-09-26" @default.
- W2029402925 title "Use of hydroxyurea in the measurement of DNA repair by the BND cellulose method" @default.
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- W2029402925 doi "https://doi.org/10.1002/em.2860020308" @default.
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