Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029432049> ?p ?o ?g. }
- W2029432049 abstract "Here we report on the expansion of a previously introduced biointerface platform based on thin spin-coated films of polystyrene-block-poly(tert-butyl acrylate) (PS-b-PtBA). Following the selective deprotection of the tert-butyl-ester groups in the PtBA skin layer by hydrolysis under acidic conditions, the activation with N-hydroxysuccinimide and the subsequent derivatization with α,ω-biotin-amine end-functionalized poly(ethylene glycol) (PEG), strepavidin was immobilized as anchor layer for specific immobilization of biotin-tagged molecules. Based on contact angle, Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and confocal fluorescence microscopy data it was shown that the polymer films were efficiently modified. The complexation of a biotin-tagged dye, as well as the nonspecific adsorption of proteins was determined by confocal fluorescence microscopy measurements. The surface coverage of the immobilized dye as a function of dye concentration in solution was found to be consistent with a high affinity-type adsorption isotherm, while the protein resistant properties were found to be similar to non-biotinylated PEG. The scope of micro- and nanostructured PS-b-PtBA biointerfaces is thereby considerably expanded to encompass the specific immobilization of biotin-tagged biomolecules." @default.
- W2029432049 created "2016-06-24" @default.
- W2029432049 creator A5015129179 @default.
- W2029432049 creator A5024800339 @default.
- W2029432049 creator A5029971314 @default.
- W2029432049 date "2010-12-01" @default.
- W2029432049 modified "2023-09-24" @default.
- W2029432049 title "Implementation of Specific Bioconjugation in Polystyrene-block -poly(tert -butyl acrylate)-Based Bio-Interfaces" @default.
- W2029432049 cites W1223638767 @default.
- W2029432049 cites W1968421448 @default.
- W2029432049 cites W1974268069 @default.
- W2029432049 cites W1981028296 @default.
- W2029432049 cites W1984699863 @default.
- W2029432049 cites W2023135762 @default.
- W2029432049 cites W2039251311 @default.
- W2029432049 cites W2054635074 @default.
- W2029432049 cites W2058096164 @default.
- W2029432049 cites W2059969653 @default.
- W2029432049 cites W2082030238 @default.
- W2029432049 cites W2083571277 @default.
- W2029432049 cites W2086418243 @default.
- W2029432049 cites W2086866459 @default.
- W2029432049 cites W2114989145 @default.
- W2029432049 cites W2139532830 @default.
- W2029432049 cites W2149023977 @default.
- W2029432049 cites W2156289700 @default.
- W2029432049 cites W2158736688 @default.
- W2029432049 doi "https://doi.org/10.1002/masy.201000036" @default.
- W2029432049 hasPublicationYear "2010" @default.
- W2029432049 type Work @default.
- W2029432049 sameAs 2029432049 @default.
- W2029432049 citedByCount "1" @default.
- W2029432049 countsByYear W20294320492013 @default.
- W2029432049 crossrefType "journal-article" @default.
- W2029432049 hasAuthorship W2029432049A5015129179 @default.
- W2029432049 hasAuthorship W2029432049A5024800339 @default.
- W2029432049 hasAuthorship W2029432049A5029971314 @default.
- W2029432049 hasConcept C121332964 @default.
- W2029432049 hasConcept C127413603 @default.
- W2029432049 hasConcept C150394285 @default.
- W2029432049 hasConcept C15920480 @default.
- W2029432049 hasConcept C160892712 @default.
- W2029432049 hasConcept C169274487 @default.
- W2029432049 hasConcept C171250308 @default.
- W2029432049 hasConcept C178790620 @default.
- W2029432049 hasConcept C185592680 @default.
- W2029432049 hasConcept C188027245 @default.
- W2029432049 hasConcept C192562407 @default.
- W2029432049 hasConcept C204628709 @default.
- W2029432049 hasConcept C21951064 @default.
- W2029432049 hasConcept C23963754 @default.
- W2029432049 hasConcept C2777516009 @default.
- W2029432049 hasConcept C2777922577 @default.
- W2029432049 hasConcept C2779989194 @default.
- W2029432049 hasConcept C2781308654 @default.
- W2029432049 hasConcept C42360764 @default.
- W2029432049 hasConcept C49853544 @default.
- W2029432049 hasConcept C521977710 @default.
- W2029432049 hasConcept C55493867 @default.
- W2029432049 hasConcept C62520636 @default.
- W2029432049 hasConcept C6556556 @default.
- W2029432049 hasConcept C88001094 @default.
- W2029432049 hasConcept C91881484 @default.
- W2029432049 hasConceptScore W2029432049C121332964 @default.
- W2029432049 hasConceptScore W2029432049C127413603 @default.
- W2029432049 hasConceptScore W2029432049C150394285 @default.
- W2029432049 hasConceptScore W2029432049C15920480 @default.
- W2029432049 hasConceptScore W2029432049C160892712 @default.
- W2029432049 hasConceptScore W2029432049C169274487 @default.
- W2029432049 hasConceptScore W2029432049C171250308 @default.
- W2029432049 hasConceptScore W2029432049C178790620 @default.
- W2029432049 hasConceptScore W2029432049C185592680 @default.
- W2029432049 hasConceptScore W2029432049C188027245 @default.
- W2029432049 hasConceptScore W2029432049C192562407 @default.
- W2029432049 hasConceptScore W2029432049C204628709 @default.
- W2029432049 hasConceptScore W2029432049C21951064 @default.
- W2029432049 hasConceptScore W2029432049C23963754 @default.
- W2029432049 hasConceptScore W2029432049C2777516009 @default.
- W2029432049 hasConceptScore W2029432049C2777922577 @default.
- W2029432049 hasConceptScore W2029432049C2779989194 @default.
- W2029432049 hasConceptScore W2029432049C2781308654 @default.
- W2029432049 hasConceptScore W2029432049C42360764 @default.
- W2029432049 hasConceptScore W2029432049C49853544 @default.
- W2029432049 hasConceptScore W2029432049C521977710 @default.
- W2029432049 hasConceptScore W2029432049C55493867 @default.
- W2029432049 hasConceptScore W2029432049C62520636 @default.
- W2029432049 hasConceptScore W2029432049C6556556 @default.
- W2029432049 hasConceptScore W2029432049C88001094 @default.
- W2029432049 hasConceptScore W2029432049C91881484 @default.
- W2029432049 hasLocation W20294320491 @default.
- W2029432049 hasOpenAccess W2029432049 @default.
- W2029432049 hasPrimaryLocation W20294320491 @default.
- W2029432049 hasRelatedWork W1965663334 @default.
- W2029432049 hasRelatedWork W1997630000 @default.
- W2029432049 hasRelatedWork W1999964549 @default.
- W2029432049 hasRelatedWork W2011149425 @default.
- W2029432049 hasRelatedWork W2023501920 @default.
- W2029432049 hasRelatedWork W2032382554 @default.
- W2029432049 hasRelatedWork W2059969653 @default.
- W2029432049 hasRelatedWork W2063482302 @default.