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- W2029458131 abstract "In addition to heterogeneity within a cell population in terms of morphologic phenotypes, there can be heterogeneity in terms of response phenotypes. Such response heterogeneity can result in misleading or false-negative experimental results because the signal produced by a minor subset of cells in a population can be difficult to detect with assays that analyze the average response of the whole population. This report describes a technique that enables the investigator (i) to identify whether a subset of a population mobilizes Ca2+ in response to a stimulus and (ii) to select and subclone cells from the responsive subset. The subcloning procedure can be performed in a manner that either controls for or disregards the kinetics of the response. The resultant subclones exhibit improved response homogeneity detected at the single cell level which is also manifested as an increased signal-to-noise ratio when the average response of the entire subclone population is studied. This technique presents an opportunity to manipulate the existing response heterogeneity toward the experimental requirements of the investigator. Results obtained with two neuronal cell lines are illustrated and other potential applications of the response-dependent subcloning procedure are discussed." @default.
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- W2029458131 date "1991-06-01" @default.
- W2029458131 modified "2023-10-14" @default.
- W2029458131 title "Isolation of subclones with enhanced Ca2+ response homogeneity by flow cytometric selection of single cells during a ligand-activated Ca2+ response" @default.
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- W2029458131 doi "https://doi.org/10.1016/s1046-2023(05)80065-9" @default.
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