Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029483604> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2029483604 endingPage "148" @default.
- W2029483604 startingPage "144" @default.
- W2029483604 abstract "Seed polyphenols, polysaccharides and lipids often interfere with or degrade RNA, restricting its yield and quality. Existing RNA isolation methods specifically alleviate one or two of these challenges, but are usually designed for a single species rather than for a broad biodiversity. Many protocols also do not eliminate chromosomal DNA, which causes false positives in gene expression studies. The method reported here addresses all of the above challenges. The concentration of the phenol blocker polyvinylpyrrolidone in the extraction buffer was optimised for use on a broader range of species. DNase I was added to eliminate chromosomal DNA and the timing of this step was optimised. Lipids were removed by centrifugation. Polysaccharides and proteins, including excess DNase I, were separated from RNA during ethanol precipitation of nucleic acids. In seeds of five plant species from four taxonomically distant families, significant amounts of RNA were isolated in less than half the time typically required by previously reported methods. Colorimetric tests showed that the isolated RNA was free from interfering contaminants. In addition, reverse transcription-PCR confirmed that the isolated RNA was of appropriate quality and integrity for gene expression studies." @default.
- W2029483604 created "2016-06-24" @default.
- W2029483604 creator A5051200704 @default.
- W2029483604 creator A5054621275 @default.
- W2029483604 date "2006-01-01" @default.
- W2029483604 modified "2023-09-23" @default.
- W2029483604 title "Isolation of high-quality RNA from polyphenol-, polysaccharide- and lipid-rich seeds" @default.
- W2029483604 cites W1233505759 @default.
- W2029483604 cites W123886925 @default.
- W2029483604 cites W1695420079 @default.
- W2029483604 cites W1963719629 @default.
- W2029483604 cites W1973447740 @default.
- W2029483604 cites W2002631115 @default.
- W2029483604 cites W2058383416 @default.
- W2029483604 cites W2081565538 @default.
- W2029483604 cites W2087644010 @default.
- W2029483604 cites W2092686336 @default.
- W2029483604 cites W2101492992 @default.
- W2029483604 cites W2125934787 @default.
- W2029483604 cites W2168973463 @default.
- W2029483604 cites W3022840074 @default.
- W2029483604 doi "https://doi.org/10.1002/pca.903" @default.
- W2029483604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16749420" @default.
- W2029483604 hasPublicationYear "2006" @default.
- W2029483604 type Work @default.
- W2029483604 sameAs 2029483604 @default.
- W2029483604 citedByCount "55" @default.
- W2029483604 countsByYear W20294836042012 @default.
- W2029483604 countsByYear W20294836042013 @default.
- W2029483604 countsByYear W20294836042014 @default.
- W2029483604 countsByYear W20294836042015 @default.
- W2029483604 countsByYear W20294836042016 @default.
- W2029483604 countsByYear W20294836042017 @default.
- W2029483604 countsByYear W20294836042018 @default.
- W2029483604 countsByYear W20294836042019 @default.
- W2029483604 countsByYear W20294836042020 @default.
- W2029483604 countsByYear W20294836042021 @default.
- W2029483604 countsByYear W20294836042022 @default.
- W2029483604 countsByYear W20294836042023 @default.
- W2029483604 crossrefType "journal-article" @default.
- W2029483604 hasAuthorship W2029483604A5051200704 @default.
- W2029483604 hasAuthorship W2029483604A5054621275 @default.
- W2029483604 hasConcept C100817775 @default.
- W2029483604 hasConcept C104317684 @default.
- W2029483604 hasConcept C112190416 @default.
- W2029483604 hasConcept C150194340 @default.
- W2029483604 hasConcept C153911025 @default.
- W2029483604 hasConcept C185592680 @default.
- W2029483604 hasConcept C24107716 @default.
- W2029483604 hasConcept C2778004101 @default.
- W2029483604 hasConcept C43617362 @default.
- W2029483604 hasConcept C552990157 @default.
- W2029483604 hasConcept C55493867 @default.
- W2029483604 hasConcept C67705224 @default.
- W2029483604 hasConcept C70899900 @default.
- W2029483604 hasConcept C86803240 @default.
- W2029483604 hasConceptScore W2029483604C100817775 @default.
- W2029483604 hasConceptScore W2029483604C104317684 @default.
- W2029483604 hasConceptScore W2029483604C112190416 @default.
- W2029483604 hasConceptScore W2029483604C150194340 @default.
- W2029483604 hasConceptScore W2029483604C153911025 @default.
- W2029483604 hasConceptScore W2029483604C185592680 @default.
- W2029483604 hasConceptScore W2029483604C24107716 @default.
- W2029483604 hasConceptScore W2029483604C2778004101 @default.
- W2029483604 hasConceptScore W2029483604C43617362 @default.
- W2029483604 hasConceptScore W2029483604C552990157 @default.
- W2029483604 hasConceptScore W2029483604C55493867 @default.
- W2029483604 hasConceptScore W2029483604C67705224 @default.
- W2029483604 hasConceptScore W2029483604C70899900 @default.
- W2029483604 hasConceptScore W2029483604C86803240 @default.
- W2029483604 hasIssue "3" @default.
- W2029483604 hasLocation W20294836041 @default.
- W2029483604 hasLocation W20294836042 @default.
- W2029483604 hasOpenAccess W2029483604 @default.
- W2029483604 hasPrimaryLocation W20294836041 @default.
- W2029483604 hasRelatedWork W1481221485 @default.
- W2029483604 hasRelatedWork W2044655846 @default.
- W2029483604 hasRelatedWork W2065704027 @default.
- W2029483604 hasRelatedWork W2080216367 @default.
- W2029483604 hasRelatedWork W2358768088 @default.
- W2029483604 hasRelatedWork W2361846046 @default.
- W2029483604 hasRelatedWork W2375093011 @default.
- W2029483604 hasRelatedWork W2800649638 @default.
- W2029483604 hasRelatedWork W2803339298 @default.
- W2029483604 hasRelatedWork W3036199006 @default.
- W2029483604 hasVolume "17" @default.
- W2029483604 isParatext "false" @default.
- W2029483604 isRetracted "false" @default.
- W2029483604 magId "2029483604" @default.
- W2029483604 workType "article" @default.