Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029501770> ?p ?o ?g. }
- W2029501770 endingPage "20" @default.
- W2029501770 startingPage "14" @default.
- W2029501770 abstract "While a large number of laboratory methods for the detection of Cryptosporidium oocysts in faecal samples are now available, their efficacy for identifying asymptomatic cases of cryptosporidiosis is poorly understood. This study was carried out to determine a reliable screening test for epidemiological studies in livestock. In addition, three molecular tests were compared to identify Cryptosporidium species responsible for the infection in cattle, sheep and horses. A variety of diagnostic tests including microscopic (Kinyoun's staining), immunological (Direct Fluorescence Antibody tests or DFAT), enzyme-linked immunosorbent assay (ELISA), and molecular methods (nested PCR) were compared to assess their ability to detect Cryptosporidium in cattle, horse and sheep faecal samples. The results indicate that the sensitivity and specificity of each test is highly dependent on the input samples; while Kinyoun's and DFAT proved to be reliable screening tools for cattle samples, DFAT and PCR analysis (targeted at the 18S rRNA gene fragment) were more sensitive for screening sheep and horse samples. Finally different PCR primer sets targetedat the same region resulted in the preferential amplification of certain Cryptosporidium species when multiple species were present in the sample. Therefore, for identification of Cryptosporidium spp. in the event of asymptomatic cryptosporidiosis, the combination of different 18S rRNA nested PCR primer sets is recommended for further epidemiological applications and also tracking the sources of infection." @default.
- W2029501770 created "2016-06-24" @default.
- W2029501770 creator A5007646248 @default.
- W2029501770 creator A5019903080 @default.
- W2029501770 creator A5030286097 @default.
- W2029501770 creator A5031515981 @default.
- W2029501770 creator A5049046161 @default.
- W2029501770 creator A5076366108 @default.
- W2029501770 date "2015-04-01" @default.
- W2029501770 modified "2023-10-12" @default.
- W2029501770 title "Comparison of diagnostic techniques for the detection of Cryptosporidium oocysts in animal samples" @default.
- W2029501770 cites W1551037622 @default.
- W2029501770 cites W1652632291 @default.
- W2029501770 cites W1915289282 @default.
- W2029501770 cites W1974349389 @default.
- W2029501770 cites W1977516453 @default.
- W2029501770 cites W1993092899 @default.
- W2029501770 cites W2001119873 @default.
- W2029501770 cites W2011283489 @default.
- W2029501770 cites W2021982621 @default.
- W2029501770 cites W2022373088 @default.
- W2029501770 cites W2046328896 @default.
- W2029501770 cites W2055043387 @default.
- W2029501770 cites W2078048454 @default.
- W2029501770 cites W2090243740 @default.
- W2029501770 cites W2093268197 @default.
- W2029501770 cites W2097356202 @default.
- W2029501770 cites W2104388919 @default.
- W2029501770 cites W2107735636 @default.
- W2029501770 cites W2113475228 @default.
- W2029501770 cites W2115182954 @default.
- W2029501770 cites W2119512144 @default.
- W2029501770 cites W2122899420 @default.
- W2029501770 cites W2126983680 @default.
- W2029501770 cites W2133223826 @default.
- W2029501770 cites W2133408300 @default.
- W2029501770 cites W2135163002 @default.
- W2029501770 cites W2137015675 @default.
- W2029501770 cites W2137339115 @default.
- W2029501770 cites W2137788264 @default.
- W2029501770 cites W2143798272 @default.
- W2029501770 cites W2144688025 @default.
- W2029501770 cites W2156838404 @default.
- W2029501770 cites W2158629393 @default.
- W2029501770 cites W2161721595 @default.
- W2029501770 cites W2166229810 @default.
- W2029501770 cites W2284474987 @default.
- W2029501770 cites W2465535226 @default.
- W2029501770 doi "https://doi.org/10.1016/j.exppara.2015.01.018" @default.
- W2029501770 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4406248" @default.
- W2029501770 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25662435" @default.
- W2029501770 hasPublicationYear "2015" @default.
- W2029501770 type Work @default.
- W2029501770 sameAs 2029501770 @default.
- W2029501770 citedByCount "31" @default.
- W2029501770 countsByYear W20295017702015 @default.
- W2029501770 countsByYear W20295017702016 @default.
- W2029501770 countsByYear W20295017702017 @default.
- W2029501770 countsByYear W20295017702018 @default.
- W2029501770 countsByYear W20295017702019 @default.
- W2029501770 countsByYear W20295017702020 @default.
- W2029501770 countsByYear W20295017702021 @default.
- W2029501770 countsByYear W20295017702022 @default.
- W2029501770 countsByYear W20295017702023 @default.
- W2029501770 crossrefType "journal-article" @default.
- W2029501770 hasAuthorship W2029501770A5007646248 @default.
- W2029501770 hasAuthorship W2029501770A5019903080 @default.
- W2029501770 hasAuthorship W2029501770A5030286097 @default.
- W2029501770 hasAuthorship W2029501770A5031515981 @default.
- W2029501770 hasAuthorship W2029501770A5049046161 @default.
- W2029501770 hasAuthorship W2029501770A5076366108 @default.
- W2029501770 hasBestOaLocation W20295017702 @default.
- W2029501770 hasConcept C104317684 @default.
- W2029501770 hasConcept C157431215 @default.
- W2029501770 hasConcept C159047783 @default.
- W2029501770 hasConcept C178790620 @default.
- W2029501770 hasConcept C185592680 @default.
- W2029501770 hasConcept C190612196 @default.
- W2029501770 hasConcept C2777563447 @default.
- W2029501770 hasConcept C2778877831 @default.
- W2029501770 hasConcept C42972112 @default.
- W2029501770 hasConcept C47698986 @default.
- W2029501770 hasConcept C49105822 @default.
- W2029501770 hasConcept C54355233 @default.
- W2029501770 hasConcept C61716771 @default.
- W2029501770 hasConcept C67905577 @default.
- W2029501770 hasConcept C71924100 @default.
- W2029501770 hasConcept C86803240 @default.
- W2029501770 hasConcept C89423630 @default.
- W2029501770 hasConcept C90856448 @default.
- W2029501770 hasConceptScore W2029501770C104317684 @default.
- W2029501770 hasConceptScore W2029501770C157431215 @default.
- W2029501770 hasConceptScore W2029501770C159047783 @default.
- W2029501770 hasConceptScore W2029501770C178790620 @default.
- W2029501770 hasConceptScore W2029501770C185592680 @default.
- W2029501770 hasConceptScore W2029501770C190612196 @default.
- W2029501770 hasConceptScore W2029501770C2777563447 @default.
- W2029501770 hasConceptScore W2029501770C2778877831 @default.