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• W2029509785 abstract "Based on a model of the active site of myosin (Ramirez, Shukla & Levy, 1978), a chemical mechanism for MgATPase and intermediate oxygen exchange is presented. In this mechanism, oxygen exchange takes place via an oxyphosphorane intermediate that undergoes double turnstile rotation (Ugi, Ramirez, Marquarding, Klusacek & Gillespie, 1971; Ramirez & Ugi, 1974. During hydrolysis by native skeletal muscle myosin, only three [18O] atoms from labelled water are rapidly incorporated into the phosphorus that is finally released to the medium as Pi; whereas, during hydrolysis by subfragment 1 (S1), which is the head of myosin, four oxygens are labelled rapidly. To explain this difference, we postulate that cleavage of the (S1)-(S2) hinge in the preparation of S1 modifies the interaction of the oxyphosphorane intermediate at the active site. This enables a normally non-exchangeable oxygen to enter the exchange process. This is consistent with our earlier interpretation to the effect that the active site and the hinge in myosin are relatively close to each other Shukla & Levy, 1977b; Shukla & Levy, 1978. We postulate that the major elements of the active site are situated on a 92 amino acid fragment, p10, isolated by Elzinga & Collins, 1977 from myosin. P10 is now known to be situated in the region that connects the head to the body of a myosin heavy chain (Lu, Sosinki, Balint & Streter, 1978). An examination of the p10 fragment for a possible point of proteolytic attack in the region of the hinge which will generate S1 revealed lysine 82. Breaking the protein chain at a point so close to the active site pocket could explain the effect of hinge cleavage on oxygen exchange. Two additional features of the present mechanism are: (1) the protonation of Pγ of a MgPα,Pγ complex of ATP, which depresses monomeric metaphosphate mediated hydrolysis, and enhances oxyphosphorane formation by addition of water to Pγ; (2) the coordination of Nτ-methylhistidinet† of actin with Mg at the active site, which activates the release of the products of hydrolysis." @default.
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• W2029509785 date "1979-02-01" @default.
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• W2029509785 title "A mechanism for the hydrolysis of MgATP by actomyosin of skeletal muscle" @default.
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• W2029509785 doi "https://doi.org/10.1016/0022-5193(79)90318-7" @default.
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