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• W2029519525 abstract "The ethyl acetate and n -butanolic subfractions of Agelanthus dodoneifolius were investigated for their antioxidant and antimyeloperoxidase (MPO) activities. The reactive oxygen species (ROS) generation was assessed by lucigenin-enhanced chemiluminescence (CL) and dichlorofluorescein- (DCF-) induced fluorescence techniques from phorbol myristate acetate- (PMA-) stimulated equine neutrophils and human myeloid cell line HL-60, respectively. In parallel, the effects of the tested subfractions were evaluated on the total MPO release by stimulated neutrophils and on the specific MPO activity by means of immunological assays. The results showed the potent activity of the butanolic subfraction, at least in respect of the chemiluminescence test (IC 50 =<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M1><mml:mrow><mml:mn>0.3</mml:mn><mml:mo>±</mml:mo><mml:mn>0.1</mml:mn></mml:mrow></mml:math> µ g/mL) and the ELISA and SIEFED assays (IC 50 =<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M2><mml:mrow><mml:mn>2.8</mml:mn><mml:mo>±</mml:mo><mml:mn>1.2</mml:mn></mml:mrow></mml:math> µ g/mL and<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M3><mml:mrow><mml:mn>1.3</mml:mn><mml:mo>±</mml:mo><mml:mn>1.0</mml:mn></mml:mrow></mml:math> µ g/mL), respectively. However, the ethyl acetate subfraction was found to be the most potent in the DCF assay as at the highest concentration, DCF fluorescence intensity decreases of about 50%. Moreover, we demonstrated that the ethyl acetate subfraction was rich in catechin (16.51%) while it was not easy to identify the main compounds in the butanolic subfraction using the UPLC-MS/MS technique. Nevertheless, taken together, our results provide evidence that Agelanthus dodoneifolius subfractions may represent potential sources of natural antioxidants and of antimyeloperoxidase compounds." @default.
• W2029519525 created "2016-06-24" @default.
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• W2029519525 date "2015-01-01" @default.
• W2029519525 modified "2023-10-01" @default.
• W2029519525 title "Evaluation of Antiradical and Anti-Inflammatory Activities of Ethyl Acetate and Butanolic Subfractions ofAgelanthus dodoneifolius(DC.) Polhill & Wiens (Loranthaceae) Using Equine Myeloperoxidase and Both PMA-Activated Neutrophils and HL-60 Cells" @default.
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• W2029519525 doi "https://doi.org/10.1155/2015/707524" @default.
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