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• W2029532553 abstract "It is difficult to simultaneously isolate and purify flavonoids and polyhydroxystilbenes due to their similar polarities. In this study, we isolated and purified these compounds from Trigonella foenum-graecum L. seed extracts using high-speed counter-current chromatography (HSCCC) in two steps with two different kinds of solvent systems. In the first step, n-hexane:ethyl acetate:methanol:water (1:5:1:5, v/v) was used as the two-phase solvent system, desoxy-rhaponticin and rhapontigenin were purified, and orientin, vitexin, isovitexin, and rhaponticin were eluted together. In the second step, ethyl acetate:water (1:1, v/v) was used as the two-phase solvent system, and orientin, vitexin, rhaponticin, and isovitexin were isolated and purified. Purities of the isolated compounds were determined using HPLC, while UV spectra, 1 H-NMR, and 13 C-NMR aided in structure identification." @default.
• W2029532553 created "2016-06-24" @default.
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• W2029532553 date "2014-02-20" @default.
• W2029532553 modified "2023-09-30" @default.
• W2029532553 title "Isolation and Purification of Six Bioactive Compounds from the Seeds of<i>Trigonella foenum-graecum</i>L. using High-Speed Counter-Current Chromatography" @default.
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• W2029532553 doi "https://doi.org/10.1080/01496395.2013.836229" @default.
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