FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029557249> ?p ?o ?g. }

• W2029557249 endingPage "144" @default.
• W2029557249 startingPage "139" @default.
• W2029557249 abstract "Abstract Low-temperature UV-visible and circular dichroism (CD) spectroscopy were employed to study the photocycle of native (wild-type, WT) bacteriorhodopsin (bR) and its mutant derivative D96N. For both bR forms, irradiation at −80°C yields the M 412 photocycle intermediate ( λ max = 412 nm). The CD spectrum matches the UV-visible absorbance, including the (vibrational) fine structure. Conversion of the M form into subsequent photocycle intermediates and the re-formation of the bR parent state are accomplished on stepwise temperature elevation. For WT bR, the CD spectrum of the M intermediate appears to be comprised of the 412 nm band and a long-wavelength component (with a broad maximum around 560 nm). This interpretation is complicated by the fact that, at −80°C, and increasing with increasing temperature, the long-wavelength CD absorption becomes negative, whereas no qualitative change of the CD band around 410 nm is observed. This new intensity interferes with the CD band of M 412 in this wavelength region. Further warming of the sample leads to the formation of another intermediate with a positive CD spectrum around 550 nm which, under our experimental conditions (low temperature, high pH), is expected to be the intermediate N 550 . The UV-visible absorption of N 550 has a similar wavelenght, but lower extinction coefficient, than that of bR 568 , whereas its CD spectrum has not yet been reported. The same experimental apparatus was employed for the D96N mutant. The UV-visible changes with increasing temperature are governed by the retarded M decay, as reported previously (Butt et al., EMBO J., 8 (1989) 1657). The “temperature-resolved” CD spectra of this mutant also reflect the retarded conversion of the M form into the bR parent state through the N form. The nearly unaffected UV-visible and CD spectra in the UV range and the concomitant changes in the long-wavelength CD signals are interpreted as the observation of an M → M′ conversion, which has been proposed by Varo and Lanyi ( Biochemistry, 30 (1991) 5008). Sodium azide addition to D96N, which accelerates the photocycle kinetics, induces a shift of the absorption band to shorter wavelengths ( λ max = 490 nm) which resembles the behaviour of bR at high pH (pH > 9.0)." @default.
• W2029557249 created "2016-06-24" @default.
• W2029557249 creator A5029424483 @default.
• W2029557249 creator A5040909240 @default.
• W2029557249 creator A5091620100 @default.
• W2029557249 date "1995-12-01" @default.
• W2029557249 modified "2023-10-12" @default.
• W2029557249 title "Circular dichroism spectroscopy of the retinal chromophore during the photocycle of bacteriorhodopsin and its D96N mutant derivative" @default.
• W2029557249 cites W12268373 @default.
• W2029557249 cites W1499129928 @default.
• W2029557249 cites W1513594391 @default.
• W2029557249 cites W1969625914 @default.
• W2029557249 cites W1981347465 @default.
• W2029557249 cites W1996206997 @default.
• W2029557249 cites W2005873601 @default.
• W2029557249 cites W2005910905 @default.
• W2029557249 cites W2008753780 @default.
• W2029557249 cites W2012318398 @default.
• W2029557249 cites W2016974353 @default.
• W2029557249 cites W2023281801 @default.
• W2029557249 cites W2036923962 @default.
• W2029557249 cites W2050292589 @default.
• W2029557249 cites W2073164938 @default.
• W2029557249 cites W2082182504 @default.
• W2029557249 cites W2110810808 @default.
• W2029557249 cites W2130653710 @default.
• W2029557249 cites W2398243329 @default.
• W2029557249 doi "https://doi.org/10.1016/1011-1344(95)07195-4" @default.
• W2029557249 hasPublicationYear "1995" @default.
• W2029557249 type Work @default.
• W2029557249 sameAs 2029557249 @default.
• W2029557249 citedByCount "6" @default.
• W2029557249 countsByYear W20295572492012 @default.
• W2029557249 countsByYear W20295572492022 @default.
• W2029557249 crossrefType "journal-article" @default.
• W2029557249 hasAuthorship W2029557249A5029424483 @default.
• W2029557249 hasAuthorship W2029557249A5040909240 @default.
• W2029557249 hasAuthorship W2029557249A5091620100 @default.
• W2029557249 hasConcept C104317684 @default.
• W2029557249 hasConcept C106159729 @default.
• W2029557249 hasConcept C111771559 @default.
• W2029557249 hasConcept C121332964 @default.
• W2029557249 hasConcept C133571119 @default.
• W2029557249 hasConcept C143065580 @default.
• W2029557249 hasConcept C146593782 @default.
• W2029557249 hasConcept C162324750 @default.
• W2029557249 hasConcept C185592680 @default.
• W2029557249 hasConcept C189542057 @default.
• W2029557249 hasConcept C192468462 @default.
• W2029557249 hasConcept C2776509742 @default.
• W2029557249 hasConcept C2778113554 @default.
• W2029557249 hasConcept C2780827179 @default.
• W2029557249 hasConcept C32891209 @default.
• W2029557249 hasConcept C41625074 @default.
• W2029557249 hasConcept C55493867 @default.
• W2029557249 hasConcept C62520636 @default.
• W2029557249 hasConcept C75473681 @default.
• W2029557249 hasConcept C8010536 @default.
• W2029557249 hasConceptScore W2029557249C104317684 @default.
• W2029557249 hasConceptScore W2029557249C106159729 @default.
• W2029557249 hasConceptScore W2029557249C111771559 @default.
• W2029557249 hasConceptScore W2029557249C121332964 @default.
• W2029557249 hasConceptScore W2029557249C133571119 @default.
• W2029557249 hasConceptScore W2029557249C143065580 @default.
• W2029557249 hasConceptScore W2029557249C146593782 @default.
• W2029557249 hasConceptScore W2029557249C162324750 @default.
• W2029557249 hasConceptScore W2029557249C185592680 @default.
• W2029557249 hasConceptScore W2029557249C189542057 @default.
• W2029557249 hasConceptScore W2029557249C192468462 @default.
• W2029557249 hasConceptScore W2029557249C2776509742 @default.
• W2029557249 hasConceptScore W2029557249C2778113554 @default.
• W2029557249 hasConceptScore W2029557249C2780827179 @default.
• W2029557249 hasConceptScore W2029557249C32891209 @default.
• W2029557249 hasConceptScore W2029557249C41625074 @default.
• W2029557249 hasConceptScore W2029557249C55493867 @default.
• W2029557249 hasConceptScore W2029557249C62520636 @default.
• W2029557249 hasConceptScore W2029557249C75473681 @default.
• W2029557249 hasConceptScore W2029557249C8010536 @default.
• W2029557249 hasIssue "3" @default.
• W2029557249 hasLocation W20295572491 @default.
• W2029557249 hasOpenAccess W2029557249 @default.
• W2029557249 hasPrimaryLocation W20295572491 @default.
• W2029557249 hasRelatedWork W1730505816 @default.
• W2029557249 hasRelatedWork W1923702724 @default.
• W2029557249 hasRelatedWork W2028123690 @default.
• W2029557249 hasRelatedWork W2029121115 @default.
• W2029557249 hasRelatedWork W2037404307 @default.
• W2029557249 hasRelatedWork W2048490182 @default.
• W2029557249 hasRelatedWork W2155425183 @default.
• W2029557249 hasRelatedWork W2412455908 @default.
• W2029557249 hasRelatedWork W2950919019 @default.
• W2029557249 hasRelatedWork W3036120673 @default.
• W2029557249 hasVolume "31" @default.
• W2029557249 isParatext "false" @default.
• W2029557249 isRetracted "false" @default.
• W2029557249 magId "2029557249" @default.
• W2029557249 workType "article" @default.