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- W2029587031 abstract "Epidemiologic data suggest that the malignant transformation of ovarian epithelium may be linked to altered steroid hormone homeostasis.Estrogen receptor-alpha, estrogen receptor-beta, progesterone receptor A, and progesterone receptor B messenger RNA and protein expression were evaluated by reverse transcriptase-polymerase chain reaction and Western blot analysis in primary cell cultures of human ovarian surface epithelium (n = 23 cultures) and Cedars-Sinai ovarian cancer (n = 23 cultures).The ratio of estrogen receptor-alpha/estrogen receptor-beta messenger RNA expression was 10 times higher in primary ovarian cancer cultures (9.94 +/- 3.90) than in normal ovarian surface epithelium cultures (1.00 +/- 0.16, P =.04). Estrogen receptor-alpha/estrogen receptor-beta protein ratio in primary ovarian cancer cultures (2.13 +/- 0.43) was twice that of normal human ovarian surface epithelium cultures (1.00 +/- 0.13, P =.05). Individual estrogen receptor-alpha and estrogen receptor-beta messenger RNA and protein expression were not significantly different. Progesterone receptor B protein levels in primary ovarian cancer cultures (2.08 +/- 0.42) were twice that of normal surface ovarian epithelium cultures (1.00 +/- 0.10, P =.04), although differences in progesterone receptor B messenger RNA and progesterone receptor A protein expression were not observed.Malignant ovarian epithelial cells demonstrated multiple alterations in the expression of sex steroid hormone receptors." @default.
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- W2029587031 date "2003-07-01" @default.
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- W2029587031 title "Estrogen and progesterone receptor subtype expression in normal and malignant ovarian epithelial cell cultures" @default.
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- W2029587031 doi "https://doi.org/10.1067/mob.2003.328" @default.
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