Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029603310> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2029603310 endingPage "274" @default.
- W2029603310 startingPage "271" @default.
- W2029603310 abstract "Oral tissue engineering aims to treat and fill tissue deficits caused by congenital defects, facial trauma, or malignant lesion surgery, as well as to study the biology of oral mucosa. The Food and Drug Administration (FDA) and the European Medicines Agency (EMA) require a large animal model to evaluate cell-based devices, including tissue-engineered oral mucosa, prior to initiating human clinical studies. Porcine oral mucosa is non-keratinized and resembles that of humans more closely than any other animal in terms of structure and composition; however, there have not been any reports on the reconstruction of a porcine oral mucosa equivalent, probably due to the difficulty to culture porcine fibroblasts. In this study, we demonstrate the feasibility of a 3D porcine oral mucosa equivalent based on a collagen-GAG-chitosan scaffold, as well as reconstructed porcine epithelium by using an amniotic membrane as support, or without any support in form of epithelial cell sheets by using thermoresponsive culture plates. Explants technique was used for the isolation of the porcine fibroblasts and a modified fibroblast medium containing 20% fetal calf serum was used for their culture. The histological and transmission electron microscopic analyses of the resulting porcine oral mucosa models showed the presence of non-keratinized epithelia expressing keratin 13, the major differentiation marker of non-keratinized oral mucosa, in all models, and the presence of newly synthesized collagen fibers in the lamina propria equivalent of the full-thickness model, indicating the functionality of porcine fibroblasts." @default.
- W2029603310 created "2016-06-24" @default.
- W2029603310 creator A5004346273 @default.
- W2029603310 creator A5007657791 @default.
- W2029603310 creator A5017143063 @default.
- W2029603310 creator A5067388024 @default.
- W2029603310 creator A5084601947 @default.
- W2029603310 date "2012-02-06" @default.
- W2029603310 modified "2023-10-14" @default.
- W2029603310 title "Feasibility of a porcine oral mucosa equivalent: A preclinical study" @default.
- W2029603310 cites W1970896690 @default.
- W2029603310 cites W1986339409 @default.
- W2029603310 cites W1988996430 @default.
- W2029603310 cites W2004917087 @default.
- W2029603310 cites W2025570573 @default.
- W2029603310 cites W2036385637 @default.
- W2029603310 cites W2057403920 @default.
- W2029603310 cites W2073118857 @default.
- W2029603310 cites W2085503510 @default.
- W2029603310 cites W2100373655 @default.
- W2029603310 cites W2146810263 @default.
- W2029603310 cites W2168695045 @default.
- W2029603310 doi "https://doi.org/10.3109/10731199.2011.644293" @default.
- W2029603310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22309108" @default.
- W2029603310 hasPublicationYear "2012" @default.
- W2029603310 type Work @default.
- W2029603310 sameAs 2029603310 @default.
- W2029603310 citedByCount "8" @default.
- W2029603310 countsByYear W20296033102014 @default.
- W2029603310 countsByYear W20296033102016 @default.
- W2029603310 countsByYear W20296033102018 @default.
- W2029603310 countsByYear W20296033102022 @default.
- W2029603310 countsByYear W20296033102023 @default.
- W2029603310 crossrefType "journal-article" @default.
- W2029603310 hasAuthorship W2029603310A5004346273 @default.
- W2029603310 hasAuthorship W2029603310A5007657791 @default.
- W2029603310 hasAuthorship W2029603310A5017143063 @default.
- W2029603310 hasAuthorship W2029603310A5067388024 @default.
- W2029603310 hasAuthorship W2029603310A5084601947 @default.
- W2029603310 hasConcept C136229726 @default.
- W2029603310 hasConcept C142724271 @default.
- W2029603310 hasConcept C203014093 @default.
- W2029603310 hasConcept C2777310793 @default.
- W2029603310 hasConcept C2779409057 @default.
- W2029603310 hasConcept C2780269544 @default.
- W2029603310 hasConcept C49892992 @default.
- W2029603310 hasConcept C529295009 @default.
- W2029603310 hasConcept C71924100 @default.
- W2029603310 hasConcept C81241287 @default.
- W2029603310 hasConcept C86803240 @default.
- W2029603310 hasConceptScore W2029603310C136229726 @default.
- W2029603310 hasConceptScore W2029603310C142724271 @default.
- W2029603310 hasConceptScore W2029603310C203014093 @default.
- W2029603310 hasConceptScore W2029603310C2777310793 @default.
- W2029603310 hasConceptScore W2029603310C2779409057 @default.
- W2029603310 hasConceptScore W2029603310C2780269544 @default.
- W2029603310 hasConceptScore W2029603310C49892992 @default.
- W2029603310 hasConceptScore W2029603310C529295009 @default.
- W2029603310 hasConceptScore W2029603310C71924100 @default.
- W2029603310 hasConceptScore W2029603310C81241287 @default.
- W2029603310 hasConceptScore W2029603310C86803240 @default.
- W2029603310 hasIssue "4" @default.
- W2029603310 hasLocation W20296033101 @default.
- W2029603310 hasLocation W20296033102 @default.
- W2029603310 hasOpenAccess W2029603310 @default.
- W2029603310 hasPrimaryLocation W20296033101 @default.
- W2029603310 hasRelatedWork W1809348865 @default.
- W2029603310 hasRelatedWork W1969124103 @default.
- W2029603310 hasRelatedWork W2026709848 @default.
- W2029603310 hasRelatedWork W2030597513 @default.
- W2029603310 hasRelatedWork W2053559534 @default.
- W2029603310 hasRelatedWork W2080483290 @default.
- W2029603310 hasRelatedWork W2168695045 @default.
- W2029603310 hasRelatedWork W2209698066 @default.
- W2029603310 hasRelatedWork W2410289811 @default.
- W2029603310 hasRelatedWork W2049246661 @default.
- W2029603310 hasVolume "40" @default.
- W2029603310 isParatext "false" @default.
- W2029603310 isRetracted "false" @default.
- W2029603310 magId "2029603310" @default.
- W2029603310 workType "article" @default.