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• W2029605325 endingPage "679" @default.
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• W2029605325 abstract "Variation in the wavelength of irradiation in infrared multiple-photon dissociation (IR-MPD) of lithium-tagged glucose-containing disaccharide ions (1-2-, 1-3-, 1-4-, and 1-6-linked isomers of both anomeric configurations) resulted in marked differences in their mass spectral fragmentation patterns. Two-dimensional plots of the fragment yield versus infrared wavelength for each mass spectral product ion were unique for each isomer and can be considered a spectral fingerprint. Individual product ions or diagnostic ratios of key product ions can be optimized at specific IR wavelengths. The technique permits both linkage position and anomeric configuration to be assigned. The ratio of the fragments derived by cleavage at the glycosidic bond (m/z 169/187) is much enhanced for beta-anomers compared to alpha-anomers. Differences in the diagnostic product ions 169 and 187 were largest in the range of 9.0-9.4 microm, where the maximum dissociation yield was observed. Conversely, at 10.6 microm, the wavelength of nontunable CO2 lasers that accompany commercial Fourier transform ion cyclotron resonance mass spectrometers, the dissociation yield was poor and anomeric differentiation was not possible. In contrast to previous studies by collision-induced dissociation, the trends in dissociation behavior between anomers using IR-MPD are significant and allow simple diagnostic rules to be established. By depositing energy into these isobaric ions via narrow-band IR excitation, the resulting internal energy can be finely controlled, thereby obtaining high reproducibility in dissociation patterns. Given the multidimensionality of variable-wavelength IR-MPD of lithiated disaccharides, it is expected that this approach can overcome some of the current limitations in isomer differentiation." @default.
• W2029605325 created "2016-06-24" @default.
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• W2029605325 date "2005-12-24" @default.
• W2029605325 modified "2023-10-14" @default.
• W2029605325 title "Differentiation of Isomers by Wavelength-Tunable Infrared Multiple-Photon Dissociation-Mass Spectrometry: Application to Glucose-Containing Disaccharides" @default.
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• W2029605325 doi "https://doi.org/10.1021/ac0519458" @default.
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