FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029608958> ?p ?o ?g. }

• W2029608958 endingPage "33893" @default.
• W2029608958 startingPage "33884" @default.
• W2029608958 abstract "IRR is a member of the insulin receptor (IR) family that does not have any known agonist of a peptide nature but can be activated by mildly alkaline medium and was thus proposed to function as an extracellular pH sensor. IRR activation by alkali is defined by its N-terminal extracellular region. To reveal key structural elements involved in alkali sensing, we developed an in vitro method to quantify activity of IRR and its mutants. Replacing the IRR L1C domains (residues 1–333) or L2 domain (residues 334–462) or both with the homologous fragments of IR reduced the receptor activity to 35, 64, and 7% percent, respectively. Within L1C domains, five amino acid residues (Leu-135, Gly-188, Arg-244, and vicinal His-318 and Lys-319) were identified as IRR-specific by species conservation analysis of the IR family. These residues are exposed and located in junctions between secondary structure folds. The quintuple mutation of these residues to alanine had the same negative effect as the entire L1C domain replacement, whereas none of the single mutations was as effective. Separate mutations of these five residues and of L2 produced partial negative effects that were additive. The pH dependence of cell-expressed mutants (L1C and L2 swap, L2 plus triple LGR mutation, and L2 plus quintuple LGRHK mutation) was shifted toward alkalinity and, in contrast with IRR, did not show significant positive cooperativity. Our data suggest that IRR activation is not based on a single residue deprotonation in the IRR ectodomain but rather involves synergistic conformational changes at multiple points. IRR is a member of the insulin receptor (IR) family that does not have any known agonist of a peptide nature but can be activated by mildly alkaline medium and was thus proposed to function as an extracellular pH sensor. IRR activation by alkali is defined by its N-terminal extracellular region. To reveal key structural elements involved in alkali sensing, we developed an in vitro method to quantify activity of IRR and its mutants. Replacing the IRR L1C domains (residues 1–333) or L2 domain (residues 334–462) or both with the homologous fragments of IR reduced the receptor activity to 35, 64, and 7% percent, respectively. Within L1C domains, five amino acid residues (Leu-135, Gly-188, Arg-244, and vicinal His-318 and Lys-319) were identified as IRR-specific by species conservation analysis of the IR family. These residues are exposed and located in junctions between secondary structure folds. The quintuple mutation of these residues to alanine had the same negative effect as the entire L1C domain replacement, whereas none of the single mutations was as effective. Separate mutations of these five residues and of L2 produced partial negative effects that were additive. The pH dependence of cell-expressed mutants (L1C and L2 swap, L2 plus triple LGR mutation, and L2 plus quintuple LGRHK mutation) was shifted toward alkalinity and, in contrast with IRR, did not show significant positive cooperativity. Our data suggest that IRR activation is not based on a single residue deprotonation in the IRR ectodomain but rather involves synergistic conformational changes at multiple points. Insulin receptor-related receptor is a member of the minifamily of three structurally related receptor tyrosine kinases that includes the insulin receptor (IR) 3The abbreviations used are: IRinsulin receptorIGF-IRinsulin-like growth factor receptor. and insulin-like growth factor receptor (IGF-IR) (1Shier P. Watt V.M. Primary structure of a putative receptor for a ligand of the insulin family.J. Biol. Chem. 1989; 264: 14605-14608Abstract Full Text PDF PubMed Google Scholar, 2Ullrich A. Bell J.R. Chen E.Y. Herrera R. Petruzzelli L.M. Dull T.J. Gray A. Coussens L. Liao Y.C. Tsubokawa M. Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.Nature. 1985; 313: 756-761Crossref PubMed Scopus (1519) Google Scholar, 3Ullrich A. Gray A. Tam A.W. Yang-Feng T. Tsubokawa M. Collins C. Henzel W. Le Bon T. Kathuria S. Chen E. Insulin-like growth factor I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity.EMBO J. 1986; 5: 2503-2512Crossref PubMed Scopus (1506) Google Scholar, 4Ebina Y. Ellis L. Jarnagin K. Edery M. Graf L. Clauser E. Ou J.H. Masiarz F. Kan Y.W. Goldfine I.D. The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling.Cell. 1985; 40: 747-758Abstract Full Text PDF PubMed Scopus (972) Google Scholar). IRR is not sensitive to insulin and two insulin-like growth factors, IGF-I and IGF-II. Moreover, multiyear studies failed to discover any IRR agonist of the protein or peptide nature. Unexpectedly, recent studies revealed an unusual property of IRR to respond to an increase of extracellular medium pH (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar, 6Deev I.E. Vasilenko K.P. Kurmangaliev E.Zh. Serova O.V. Popova N.V. Galagan Y.S. Burova E.B. Zozulya S.A. Nikol'skii N.N. Petrenko A.G. Effect of changes in ambient pH on phosphorylation of cellular proteins.Dokl. Biochem. Biophys. 2006; 408: 184-187Crossref PubMed Scopus (15) Google Scholar). This finding suggests that the hydroxyl anion may represent a natural agonist of IRR (7Petrenko A.G. Zozulya S.A. Deyev I.E. Eladari D. Insulin receptor-related receptor as an extracellular pH sensor involved in the regulation of acid-base balance.Biochim. Biophys. Acta. 2013; 1834: 2170-2175Crossref PubMed Scopus (30) Google Scholar). The precise mechanism of the IRR pH sensitivity is not known yet and participation of other ubiquitously present proteins or lipids cannot be excluded. insulin receptor insulin-like growth factor receptor. The physiological importance of IRR as a component of the acid-base homeostasis machinery is supported by complementary in vitro and in vivo studies (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar, 8Deyev I.E. Rzhevsky D.I. Berchatova A.A. Serova O.V. Popova N.V. Murashev A.N. Petrenko A.G. Deficient Response to Experimentally Induced Alkalosis in Mice with the Inactivated insrr Gene.Acta Naturae. 2011; 3: 114-117Crossref PubMed Google Scholar). First, unlike its ubiquitously distributed homologs IR and IGF-IR, IRR is located in only specific cell sets within some tissues that come in contact with extracorporeal fluids of extreme pH such as kidney, pancreas, and stomach, as reviewed in Ref. 7Petrenko A.G. Zozulya S.A. Deyev I.E. Eladari D. Insulin receptor-related receptor as an extracellular pH sensor involved in the regulation of acid-base balance.Biochim. Biophys. Acta. 2013; 1834: 2170-2175Crossref PubMed Scopus (30) Google Scholar. Second, in transfected cells, IRR (but not IR or IGF-IR) can be activated by external application of mildly alkaline medium independently of its ionic composition, and this effect is dose-dependent (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar). Third, IRR knock-out mice, seemingly healthy under standard environmental conditions, fail to respond properly to an experimentally introduced alkali challenge by kidney secretion of excessive base as bicarbonate (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar, 8Deyev I.E. Rzhevsky D.I. Berchatova A.A. Serova O.V. Popova N.V. Murashev A.N. Petrenko A.G. Deficient Response to Experimentally Induced Alkalosis in Mice with the Inactivated insrr Gene.Acta Naturae. 2011; 3: 114-117Crossref PubMed Google Scholar). Thus, the knock-out animal data provide a strong support that the in vitro findings of alkali-dependent IRR activation are physiologically relevant. It appears that, despite strong amino acid sequence homology, the IRR function and activation mechanism are quite distinct from those of IR and IGF-IR and therefore represent a special interest. Generally, receptor tyrosine kinases are activated due to ligand-induced dimerization followed by autophosphorylation of intracellular catalytic domains (9De Meyts P. Insulin and its receptor: structure, function and evolution.Bioessays. 2004; 26: 1351-1362Crossref PubMed Scopus (243) Google Scholar, 10Schlessinger J. Cell signaling by receptor tyrosine kinases.Cell. 2000; 103: 211-225Abstract Full Text Full Text PDF PubMed Scopus (3524) Google Scholar). In the case of the insulin receptor minifamily, the receptor monomers are predimerized by disulfide bonds, and ligand binding induces a major conformational change that eventually results in the catalytic subunits approaching each other (11Hubbard S.R. Structural analysis of receptor tyrosine kinases.Prog. Biophys. Mol. Biol. 1999; 71: 343-358Crossref PubMed Scopus (158) Google Scholar, 12Ward C.W. Lawrence M.C. Similar but different: ligand-induced activation of the insulin and epidermal growth factor receptor families.Curr. Opin. Struct. Biol. 2012; 22: 360-366Crossref PubMed Scopus (30) Google Scholar). Due to proteolytic processing, mature receptor monomers consist of disulfide-linked hydrophilic extracellular α-subunit and membrane-bound β-subunit with a phosphotyrosine kinase domain (13De Meyts P. The insulin receptor: a prototype for dimeric, allosteric membrane receptors?.Trends Biochem. Sci. 2008; 33: 376-384Abstract Full Text Full Text PDF PubMed Scopus (125) Google Scholar). We have previously shown that the pH sensitivity of IRR is defined by its extracellular region, which, similarly to other members of the IR minifamily, contains two leucine-rich repeat domains, named L1 and L2, joined by the cysteine-rich C-domain, and three C-terminal fibronectin type III repeats 1 (FnIII-1, FnIII-2, and FnIII-3) (1Shier P. Watt V.M. Primary structure of a putative receptor for a ligand of the insulin family.J. Biol. Chem. 1989; 264: 14605-14608Abstract Full Text PDF PubMed Google Scholar, 13De Meyts P. The insulin receptor: a prototype for dimeric, allosteric membrane receptors?.Trends Biochem. Sci. 2008; 33: 376-384Abstract Full Text Full Text PDF PubMed Scopus (125) Google Scholar, 14Bajaj M. Waterfield M.D. Schlessinger J. Taylor W.R. Blundell T. On the tertiary structure of the extracellular domains of the epidermal growth factor and insulin receptors.Biochim. Biophys. Acta. 1987; 916: 220-226Crossref PubMed Scopus (117) Google Scholar). The site of the endogenous cleavage lies within the second FnIII repeat. The qualitative analysis of IR/IRR chimeras revealed involvement of several extracellular domains in IRR alkali sensing with the primary role of L1C domains (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar). To get further insight into the mechanism of IRR activation, we have now developed an in vitro assay of IRR autophosphorylation that could be quantitated. By analyzing a set of IR/IRR chimeras and IRR point mutants, we have identified key motifs and amino acid residues involved in IRR alkali sensing and estimated their contribution to IRR activation. The chimeras of human IRR (GenBankTM accession no. NP_055030) and IR (GenBankTM accession nos. BC117172) with full or partial ectodomain swapping were obtained by cloning using PCR strategy as described in Ref. 5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar. For generation of additional constructions with point mutations or with tyrosine kinase domain swapping, we used megaprimer PCR approach with mutated oligonucleotides. The following primers were used for generation of TK(N) chimera in the tyrosine kinase domain by megaprimer PCR approach: IRR_TK_N2, 5′-GGGAGCAGATCTCGATAATCCGAGAGCTGGGGCAGGGC-3′; IRR_TK_N1, 5′-TCTTGAGGTCCCCACGGGTCATCAGCTCCATCACCACCA-3′. The following primers were used to generate a TK(C) chimera in the tyrosine kinase domain by megaprimer PCR approach: IRR_TK_C2, 5′-TCTGGTCATCATGGAGTTAATGGCTCACGGAGACCTGAAGAG-3′; IRR_TK_C1, 5′-CCAAGATGAGGCCAACCTTCACACACATTCTGGACAGCATAC-3′. The following primers were used to generate a TK chimera in the tyrosine kinase domain by megaprimer PCR approach: IRR_TK_N2 - 5′-GGGAGCAGATCTCGATAATCCGAGAGCTGGGGCAGGGC-3′; IRR_TK_C1, 5′-CCAAGATGAGGCCAACCTTCACACACATTCTGGACAGCATAC-3′. The following primers were used to generate mutations in L1C domains of IRR: HK_to_AA, 5′-CGGAATTCCAGCAGCATATTCTGCGCCGCCTGCGAGGGGCTGTGCCCT-3′(contains artificial EcoRI site in IRR sequence); H_to_A, 5′-CGGAATTCCAGCAGCATATTCTGCGCCAAGTGCGAGGGGCTGTGCCCT-3′; K_to_A, 5′-CGGAATTCCAGCAGCATATTCTGCCACGCCTGCGAGGGGCTGTGCCCT-3′;R_to_A, 5′-GCAGCCAGCCAGAAGACCCTGCCGCCTGTGTAGCTTGCCGC-3′; G_to_A, 5′-GGTGTGCTGGGTGCTGCTGCCGAGCCCTGTGCCAAGACC-3′; L_to_A, 5′-CACACGCACAGCCCCACGCGCCACGGCCCCAAGTGCAGG-3′. Primers HK_to_AA, H_to_A, and K_to_A were used to generate of a double mutant or single mutations in His-318 and Lys-319 with artificial EcoRI, which coded identical IRR sequence, by megaprimer PCR approach. To generate other mutations in Leu-135, Gly-188, and Arg-244, we used primers L_to_A, G_to_A, R_to_A, by megaprimer PCR approach. The sequences of all constructs were confirmed by DNA sequencing. HEK 293 cells were cultured in DMEM supplemented with 10% fetal bovine serum (Hyclone), 1% penicillin/streptomycin, and 2 mm l-glutamine. HEK 293 cells were transfected by unifectin-56 (Unifect Group, Moscow, Russia), according to the manufacturer's instructions. Confluent monolayers of cells on culture dishes were washed with serum-free F-12 and incubated for 3 h in serum-free F-12 containing 1% penicillin/streptomycin. The cells were further incubated in PBS with 60 mm Tris-HCl of the indicated pH or supplemented with 100 nm insulin for 10 min at room temperature and lyzed in the loading SDS-electrophoresis buffer. HEK 293 cells were transfected with plasmids encoding IRR or mutant proteins with a C-terminal His6 tag. In 2 days after transfection, the cells were washed by serum-free F-12 and incubated for 3 h in serum-free F-12 containing 1% penicillin/streptomycin. The cells were then lyzed in ice-cold lysis buffer (50 mm Hepes-KOH, pH 7.4, 150 mm NaCl, 1% Triton X-100, and 1 mm PMSF). Cell extracts were centrifuged at 15,000 × g for 15 min, and the supernatants were incubated with Ni-NTA-agarose (Qiagen) at 4 °C for 90 min. The matrices were further washed three times with the lysis buffer and incubated with cold elution buffer (100 mm Hepes-KOH, pH 7.4, 150 mm NaCl, 100 mm imidazole, 15 mm MgCl2, 0.1% Triton X-100, and 1 mm Na3VO4) at 4 °C for 90 min. Two samples of the eluates (80 μl) were supplemented with 20 μl of 1 m Tris-HCl, pH 7.1 or 9.1 (at +25 °C), and incubated for 30 min on ice. Furthermore, ATP was added to the final concentration of 100 nm, and eluates were placed at 25 °C for 5 min. To stop autophosphorylation reaction, 5× SDS-loading buffer was added. Rabbit anti-IR/IRR antibodies were raised against the IRR C-terminal cytoplasmic domain (amino acids 961–1297) expressed in bacteria as a GST-fusion protein. The anti-pIR/IRR antibodies were raised against KLH-coupled peptide CGMTRDVpYETDpYpYRKGGKGL from the activation loop of IRR (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar). The lysates and eluates were separated by electrophoresis in 8% SDS-polyacrylamide gel followed by transfer onto ECL-grade nitrocellulose (Amersham Biosciences) as described (15Deyev I.E. Petrenko A.G. Regulation of CIRL-1 proteolysis and trafficking.Biochimie (Paris). 2010; 92: 418-422Crossref PubMed Scopus (17) Google Scholar). For detection of phosphorylated IRS-1 and actin, we used rabbit anti-pIRS-1(Tyr-896) (Sigma) and mouse anti-actin (Chemicon) antibodies. The bound antibodies were detected with anti-rabbit or anti-mouse HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) following visualization with SuperSignal Chemiluminescent Substrate System (Pierce). For quantitative analysis of the Western blots, Molecular Imager VersaDoc MP4000 (Bio-Rad) was used, together with with ImageJ and GraphPad 5 software. Hill plots were made using transform functions in GraphPad software with approximations of saturations signals from the autophosphorylation data. We have previously determined the role of IRR ectodomain and its fragments in its alkali sensitivity by the analysis of exogenously expressed IR/IRR chimeras (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar). The obtained data revealed the involvement of multiple motifs but was qualitative only because the actual amount of surface-expressed receptors was not known. We therefore designed the in vitro test system that would allow us to quantify the receptor activation (i.e. autophosphorylation) as a function of the ligand concentration. This test was based on the assay performed with immunoprecipitated solubilized insulin receptors (16Grigorescu F. White M.F. Kahn C.R. Insulin binding and insulin-dependent phosphorylation of the insulin receptor solubilized from human erythrocytes.J. Biol. Chem. 1983; 258: 13708-13716Abstract Full Text PDF PubMed Google Scholar). We prepared plasmid constructs encoding C-terminally His-tagged IR and IRR receptors and used them to transfect HEK 293 cells. The expressed proteins were precipitated on Ni-NTA resin and further tested by the in vitro autophosphorylation assay. The matrices were incubated in Tris-HCl buffer with neutral or alkaline pH or with 100 nm insulin and further analyzed by blotting with antibodies against IR/IRR (anti-IR/IRR) and their phosphorylated form (anti-pIR/IRR). Our preliminary experiments indicated that the in vitro autophosphorylation assay worked well for IR but not for IRR. To test the possibility that the identity of intracellular catalytic domain is important for this assay, two chimeras of IRR and IR were prepared (Fig. 1A, left panel). The first chimera contained the entire extracellular region of IRR fused with the rest of IR (IRR/IR), and the second one contained the entire extracellular region of IR fused with the rest of IRR (IR/IRR). IR and IRR/IR proteins showed strong response to insulin and alkaline medium, respectively (Fig. 1A, right panel). However, we could not detect any phosphorylation of precipitated IRR and IR/IRR receptors. Essentially, similar results were obtained in the same assay with the addition of 15 mm MnCl2 to the autophosphorylation reaction (data not shown). It should be noted that the IR/IRR construct, similarly to endogenous IR, was autophosphorylated in intact cells upon the insulin treatment (Fig. 1B). We also prepared chimeras where only catalytic tyrosine kinase domain or its fragments of IR were introduced into IRR (Fig. 1C, left panel). The chimera IRR-TK(N) contained the IR fragment from Arg-1027 to Met-1103, the other chimera IRR-TK(C) contained the homologous IR sequence from Thr-1104 to Phe-1283, and the third chimera IRR-TK contained the entire catalytic tyrosine kinase domain (supplemental Fig. S1). The autophosphorylation in vitro assay of all three chimeras revealed their response to alkaline medium (Fig. 1C, right panel). We concluded that this autophosphorylation test could be used to analyze IRR activation but only when its catalytic domain is replaced with the one of IR or its fragments. Our data are in agreement with the previously reported deficiency of autophosphorylation of yeast-expressed IRR cytoplasmic domain. Under the same conditions, the highly homologous intracellular domains of IR and IGF-IR were autophosphorylated quite well, and the IRR deficiency could be rescued by entire IGF-IR tyrosine kinase domain replacement or just its C-terminal part (17Klammt J. Garten A. Barnikol-Oettler A. Beck-Sickinger A.G. Kiess W. Comparative analysis of the signaling capabilities of the insulin receptor-related receptor.Biochem. Biophys. Res. Commun. 2005; 327: 557-564Crossref PubMed Scopus (8) Google Scholar). The reason of this phenomenon is not clear because the multiple alignment of full IRR, and IR sequences revealed the highest homology precisely within the catalytic tyrosine kinase domain ∼80%. A simple explanation is that the catalytic property is sensitive to the detergent extraction. An alternative possibility is that IRR autophosphorylation in cells requires additional intracellular components such as adaptor proteins or lipids that act as co-factors. Consequently, all chimeric constructs, tested by in vitro phosphorylation assay, included either entire IR catalytic domain or its fragment, depending on expression efficiency of the specific mutant. Three chimeras, containing L1C or L2 domains of IRR, or both substituted with the corresponding sequences of IR, were prepared, and their in vitro activity was measured (Fig. 2A). To normalize the data for the actual receptor protein amount, the blotting membranes, after staining with anti-pIR/IRR antibodies, were stripped with stripping solution (50 mm Tris-HCl, pH 6.8, 2% (w/v) SDS, 100 mm 2-mercaptoethanol) and reprobed with anti-IR/IRR antibodies (Fig. 2B). The differences of normalized phosphosignals after alkaline and neutral pH treatment for L1C-TK(N) or L2-TK(N) chimeras were compared with the same data for IRR-TK(N), which was used as the 100% reference. For L1C-TK(N), L2-TK(N), and L1CL2-TK(N) chimeras, we observed a significant reduction of autophosphorylation activity to 35 ± 8%, 64 ± 3%, and 7 ± 3%, respectively, as compared with chimera with the intact IRR ectodomain (Fig. 2C). Because L1C substitution produced the strongest effect on IRR sensitivity to alkali, we attempted to identify specific residues in L1C domain that were critically important. We analyzed the multiple alignment of IR, IGF-IR, and IRR sequences from different species (supplemental Fig. S2) by searching for the residues that were conserved in IRR among species but differed significantly from those of IR or IGF-IR, e.g. by polarity or charge. Five amino acid residues in L1C domains of IRR were identified, namely Leu-135 (versus Thr in IR and IGF-IR), Gly-188 (versus Lys or Met in IR and IGF-IR), Arg-244 (versus Thr or Ser in IR and IGF-IR), His-318, and Lys-319 (versus TP, IP, or SP in IR and IGF-IR). To test the importance of the identified amino acid residues for pH sensing, we prepared a set of constructs with all five residues or only some of them mutated to alanine in addition to a swapped L2 domain (Fig. 2A, the fifth from above scheme). Alanine mutations are traditionally considered as the least disturbing intervention into the receptor structure. These mutants were expressed at comparable levels and processed correctly. Also, all of them showed some level of the receptor activity, suggesting there was no serious misfolding problem. The quintuple mutant LGR_HK_L2-TK(N) preserved only 7 ± 2% of the original full IRR ectodomain construct activity (Fig. 2C). These five mutations appeared to be as effective as the replacement of the entire L1C domains (Fig. 2C). To address the role of specific residues, the activity of constructs with double mutation of two ionic residues His-318 and Lys-319 changed to AA or triple mutation of Leu-135, Gly-188, and Arg-244, together with L2 substitution was further measured (Fig. 2A, the sixth and seventh from above, respectively). Surprisingly, the HK mutation produced low positive effect on IRR activity, as compared with the L2 chimera. However, the triple LGR mutation decreased the activity to only 40 ± 4%, indicating synergism of HK and LGR mutations (Fig. 2C). Because the HK mutation increased the activity of L2 chimera, a set of similar constructs with also single mutations in His-318 and Lys-319 without L2 swapping was analyzed and compared (Fig. 3A). The HK mutation decreased the activity by ∼29 ± 8%, whereas single mutations of H or K produced less pronounced effect with activity decrease by 21 ± 5% or 25 ± 9%, respectively. The triple LGR mutation appeared to be more efficient with ∼42 ± 8% reduction. The quintuple mutant activity was essentially the same as of L1C chimera (Fig. 3B). We may therefore conclude that the combination of these five amino acid residues play a key role in the L1C pH-sensing machinery. We further tested the effect of individual Leu-135, Gly-188, and Arg-244 mutations on the HK and L2 background (Fig. 4A). None of these were critical, Leu-135, Arg-244, or Gly-188 mutations decreased the phosphorylation level of chimeras to 43 ± 7%, 39 ± 2%, and 25 ± 6%, respectively, with the IRR/IR activity as 100% (Fig. 4B). To understand the mechanism of the loss of IRR alkali sensing activity in the mutants, we analyzed the pH dependence of their activation. This assay was performed as described previously (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar) in live transfected cells to ensure “native” environment for expressed proteins. All chimeras therefore contained the native intracellular part of IRR. The transfected cells were incubated with a set of Tris-buffered physiological saline solutions with varied pH from 7.4 to 9.4 in small increments. Lysates of transfected cells were directly analyzed by Western blotting with anti-pIR/IRR antibodies and signal quantitation with the Molecular Imager. The blots were further stripped and stained again with anti-IR/IRR C-terminal end antibodies. The ratio of integral density of the phosphorylated receptor (pIR/IRR signal) to the total receptor (IR/IRR antibody signal) was plotted versus pH. The effect is shown as percentile of the strongest signal at pH 9.4 for each mutant. Wild-type IRR together with chimeras L1C-IRR and L2-IRR (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar) and two newly constructed chimeras LGR_L2-IRR and LGR_HK_L2-IRR with three or five point mutations in L1C domain of IRR and L2 replacement (Fig. 5A) were analyzed in this test. In the case of wild-type IRR, the activation curve in logarithmic (effect versus pH) coordinates reflected a sharp sigmoid-shaped response with a half effect at pH 8.5–8.6 (EC50, 4.3 ± 0.5 μm), saturation at about pH 9.2, and the Hill slope 2.3 ± 0.3, as calculated by GraphPad software with nonlinear regression “one site-specific binding with Hill slope” (Fig. 5B). All mutant constructs produced pH dependence curves with a different shape and no signs of saturation were observed (Fig. 5B and see Fig. 6A). EC50 for all tested mutant constructs were shifted toward alkalinity. In linear (effect versus [OH−]) coordinates, all mutants, but not IRR, showed first-order kinetics of their activation that could be fitted by straight lines. The Hill plots with average values for each pH points and each constructs (Fig. 6B) clearly showed higher cooperativity of wild-type IRR activation (2.3 ± 0.1) in comparison with the mutants (1.3 ± 0.2 for LGR_HK_L2, 1.5 ± 0.1 for LGR_L2, 1.6 ± 0.1 for L1C, and 1.2 ± 0.1 for L2).FIGURE 6The pH dependence of IRR and its mutant activation (shown in Fig. 5B) in special coordinates. A, phosphosignals were normalized to the anti-IR/IRR signals and plotted versus hydroxyl ion concentration on a linear scale. On the y axis, the mean value of each mutant activation at pH 9.4 represents 100%. All data were fitted with linear regression by GraphPad software (version 5). The values of EC50 were calculated by GraphPad software (version 5). Values are means ± S.E. (n = 4). B, Hill plots of mutants activation by alkali. For this analysis, GraphPad software (version 5) was used, and average of replicates for each pH were plotted with linear regression analysis.View Large Image Figure ViewerDownload Hi-res image Download (PPT) We had previously shown that IRR activation can trigger insulin-like cellular response, in particular, activation of IRS-1. We therefore tested whether significant reduction of the IRR pH-sensing property would also restrain a cellular signaling response. For this purpose, wild-type IRR and its L1CL2 chimera with partial swapping of L1CL2 first three domains of IR with the rest of IRR, that shows only a minor response to alkali treatment (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar), were expressed in HEK cells. The cells were treated with F-12 with pH 7.4 or 9.0 adjusted by 60 mm Tris-HCl for 20 min at 37 °C and then lyzed and Western blotted with anti-phosphoIRS-1 antibody, with anti-IR/IRR and actin antibodies as controls of IRR expression and protein loading. The resulting data indicated only a minor activation of endogenous IRS-1 in the cells expressing the chimera as compared with wild-type IRR-expressing cells (Fig. 7). The mechanism of IRR activation by alkali is of special interest because of IRR high structural homology with two other members of the IR family. Their activation requires binding of hormone to predimerized receptor subunits followed by a major conformational change eventually leading to autophosphorylation of intracellular kinase domains (18Siddle K. Ursø B. Niesler C.A. Cope D.L. Molina L. Surinya K.H. Soos M.A. Specificity in ligand binding and intracellular signalling by insulin and insulin-like growth factor receptors.Biochem. Soc. Trans. 2001; 29: 513-525Crossref PubMed Scopus (129) Google Scholar). Yet, the precise mechanism of IR and IGF-IR rearrangement upon ligand binding remains enigmatic. Understanding of hormone-free activation of IRR is of interest as a rare example of pH-induced signaling. It may also shed light on complex events involved in insulin and insulin-like growth factor action. In this study, we searched for specific differences between IRR and IR that, being relatively minor, generate a unique property of alkali sensing. Previously, we demonstrated that IRR pH sensing is defined by its ectodomain (5Deyev I.E. Sohet F. Vassilenko K.P. Serova O.V. Popova N.V. Zozulya S.A. Burova E.B. Houillier P. Rzhevsky D.I. Berchatova A.A. Murashev A.N. Chugunov A.O. Efremov R.G. Nikol'sky N.N. Bertelli E. Eladari D. Petrenko A.G. Insulin receptor-related receptor as an extracellular alkali sensor.Cell Metab. 2011; 13: 679-689Abstract Full Text Full Text PDF PubMed Scopus (71) Google Scholar). Because IR and IRR have the same domain structure of their extracellular regions, we swapped their large independent fragments and tested the response of chimeras to alkali stimulation in transfected cells. It appeared that essentially all domains of IRR extracellular region were involved in alkali sensing, but their role could not be estimated accurately due to method limitations. We now developed the in vitro assay that allowed us to quantify activity of IRR chimeric mutants with IR swapped domains. We determined that L1C and L2 domains of IRR are most important, their replacement with the corresponding IR fragments decreased IRR activity to ∼35 and 64%, respectively (Fig. 2). We next aimed to identify specific amino acid residues within L1C domains that could be of key importance. By the multiple alignment analysis of the insulin receptor family sequences in several species, five residues in L1C domains were identified that were conserved in species but differed substantially between IRR and IR and IGF-IR, namely Leu-135 (versus Thr in IR and IGF-IR), Gly-188 (versus Lys or Met in IR and IGF-IR), Arg-244 (versus Thr or Ser in IR and IGF-IR), His-318 and Lys-319 (versus TP, IP, or SP in IR and IGF-IR) (Fig. 2 and supplemental Fig. S2). As the tertiary structure of IRR is not known, the position of these residues can only be predicted within the known crystal structure of IR (Fig. 8). It should be noted that amino acids in the same positions do not map to binding sites for insulin or IGF-I in IR and IGF-IR, which were mapped by mutagenesis scanning approach (supplemental Fig. S2) (19Whittaker J. Groth A.V. Mynarcik D.C. Pluzek L. Gadsbøll V.L. Whittaker L.J. Alanine scanning mutagenesis of a type 1 insulin-like growth factor receptor ligand binding site.J. Biol. Chem. 2001; 276: 43980-43986Abstract Full Text Full Text PDF PubMed Scopus (76) Google Scholar, 20Williams P.F. Mynarcik D.C. Yu G.Q. Whittaker J. Mapping of an NH2-terminal ligand binding site of the insulin receptor by alanine scanning mutagenesis.J. Biol. Chem. 1995; 270: 3012-3016Abstract Full Text Full Text PDF PubMed Scopus (90) Google Scholar, 21Whittaker J. Sørensen H. Gadsbøll V.L. Hinrichsen J. Comparison of the functional insulin binding epitopes of the A and B isoforms of the insulin receptor.J. Biol. Chem. 2002; 277: 47380-47384Abstract Full Text Full Text PDF PubMed Scopus (43) Google Scholar, 22Mynarcik D.C. Williams P.F. Schaffer L. Yu G.Q. Whittaker J. Analog binding properties of insulin receptor mutants. Identification of amino acids interacting with the COOH terminus of the B-chain of the insulin molecule.J. Biol. Chem. 1997; 272: 2077-2081Abstract Full Text Full Text PDF PubMed Scopus (34) Google Scholar, 23Rouard M. Bass J. Grigorescu F. Garrett T.P. Ward C.W. Lipkind G. Jaffiole C. Steiner D.F. Bell G.I. Congenital insulin resistance associated with a conformational alteration in a conserved β-sheet in the insulin receptor L1 domain.J. Biol. Chem. 1999; 274: 18487-18491Abstract Full Text Full Text PDF PubMed Scopus (19) Google Scholar, 24Nakae J. Morioka H. Ohtsuka E. Fujieda K. Replacements of leucine 87 in human insulin receptor alter affinity for insulin.J. Biol. Chem. 1995; 270: 22017-22022Abstract Full Text Full Text PDF PubMed Scopus (17) Google Scholar, 25Schumacher R. Mosthaf L. Schlessinger J. Brandenburg D. Ullrich A. Insulin and insulin-like growth factor-1 binding specificity is determined by distinct regions of their cognate receptors.J. Biol. Chem. 1991; 266: 19288-19295Abstract Full Text PDF PubMed Google Scholar, 26Rentería M.E. Gandhi N.S. Vinuesa P. Helmerhorst E. Mancera R.L. A comparative structural bioinformatics analysis of the insulin receptor family ectodomain based on phylogenetic information.PLoS One. 2008; 3: e3667Crossref PubMed Scopus (39) Google Scholar) or by crystal structure of contacts between insulin and L1 domain of insulin receptor (27Menting J.G. Whittaker J. Margetts M.B. Whittaker L.J. Kong G.K. Smith B.J. Watson C.J. Záková L. Kletviková E. Jiráček J. Chan S.J. Steiner D.F. Dodson G.G. Brzozowski A.M. Weiss M.A. Ward C.W. Lawrence M.C. How insulin engages its primary binding site on the insulin receptor.Nature. 2013; 493: 241-245Crossref PubMed Scopus (282) Google Scholar). According to the structure of IR ectodomain, the five mutated residues are not concentrated in a cluster and, most likely, are exposed to the aqueous phase (Fig. 7). Except for Leu-135, they are located in predicted loops between primary folds, raising the possibility that IRR activation involves relative shifts of structural domains. In particular, Lys-319 in IRR would allow higher flexibility of the junction than P in the corresponding position of IR. Mutation of all the five residues was equivalent to replacement of L1C domains; together with the L2 swapping, it resulted in almost complete loss of the receptor pH sensitivity. The vicinal His-318 and Lys-319 were of special interest since those are ionized residues and thus could be at the heart of the pH sensor. Unexpectedly, His-318/Lys-319 double mutant on the background of the L2 swap showed higher activity (80%) than the L2 swap only chimera, perhaps by relieving structural constraints due to L2 replacement. However, the triple LGR mutation was less potent than the quintuple one. Also, the HK mutation without L2 replacement decreased the receptor activity. Thus, vicinal HK are important for pH sensing but are not likely to be the key pH-sensing residues. Single residue mutations of Leu-135, Gly-188, and Arg-244 indicate that they all are involved in IRR pH sensing. However, none of these are of critical importance. Their contribution is not precisely additive suggesting they are part of the same structural component of the IRR activation machinery. The L2 domain is likely to be another independent unit of this mechanism as L2 replacement with the IR L2 domain produces additive effect with point mutations in L1C domains. To confirm the results of the in vitro tests and to determine the pH dependence of the mutant activation, IRR and four mutant constructs (L1C or L2 swap only, L2 plus triple mutation of Leu-135, Gly-188, and Arg-244, and L2 plus quintuple mutation) were transiently expressed and tested in intact cells without receptor solubilization. In contrast with wild-type IRR, the pH sensitivity of all three mutants shifted toward alkalinity, and no saturation was observed at pH values compatible with cell viability. The shape of the pH dependence curves indicated the absence of cooperativity in contrast with strong positive cooperativity of wild-type IRR. Also in intact cells, we showed that the L1CL2 mutant, in parallel with its low alkaline pH sensitivity, produced very minor IRS-1 activation in contrast with wild-type IRR. Altogether, our data suggest that IRR activation mechanism is not based on the mere deprotonation of a single residue in a “pH-sensing center” but represents the combination of at least two distinct structural changes that act synergistically. This cooperativity of the conformational change in the IRR ectodomain upon deprotonation may be based, at least partially, on the symmetrical involvement of two IRR subunits that are complexed as a head-to-foot λ-shaped dimer. Download .zip (.02 MB) Help with zip files" @default.
• W2029608958 created "2016-06-24" @default.
• W2029608958 creator A5010921421 @default.
• W2029608958 creator A5019023007 @default.
• W2029608958 creator A5033026989 @default.
• W2029608958 creator A5035044300 @default.
• W2029608958 creator A5037124878 @default.
• W2029608958 creator A5052663125 @default.
• W2029608958 creator A5072573381 @default.
• W2029608958 creator A5078789225 @default.
• W2029608958 date "2013-11-01" @default.
• W2029608958 modified "2023-09-28" @default.
• W2029608958 title "Structural Determinants of the Insulin Receptor-related Receptor Activation by Alkali" @default.
• W2029608958 cites W1487174454 @default.
• W2029608958 cites W1654852813 @default.
• W2029608958 cites W1896621431 @default.
• W2029608958 cites W1931843124 @default.
• W2029608958 cites W1972103863 @default.
• W2029608958 cites W1972440263 @default.
• W2029608958 cites W1979532631 @default.
• W2029608958 cites W1987880730 @default.
• W2029608958 cites W1991710558 @default.
• W2029608958 cites W1999192227 @default.
• W2029608958 cites W2008897131 @default.
• W2029608958 cites W2012654422 @default.
• W2029608958 cites W2028311537 @default.
• W2029608958 cites W2042258388 @default.
• W2029608958 cites W2047858844 @default.
• W2029608958 cites W2056426338 @default.
• W2029608958 cites W2059082073 @default.
• W2029608958 cites W2059225311 @default.
• W2029608958 cites W2076104903 @default.
• W2029608958 cites W2077630059 @default.
• W2029608958 cites W2079659159 @default.
• W2029608958 cites W2081029149 @default.
• W2029608958 cites W2084338782 @default.
• W2029608958 cites W2085466569 @default.
• W2029608958 cites W2094654898 @default.
• W2029608958 cites W2122567567 @default.
• W2029608958 cites W4211075026 @default.
• W2029608958 cites W7552740 @default.
• W2029608958 doi "https://doi.org/10.1074/jbc.m113.483172" @default.
• W2029608958 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3837130" @default.
• W2029608958 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24121506" @default.
• W2029608958 hasPublicationYear "2013" @default.
• W2029608958 type Work @default.
• W2029608958 sameAs 2029608958 @default.
• W2029608958 citedByCount "22" @default.
• W2029608958 countsByYear W20296089582015 @default.
• W2029608958 countsByYear W20296089582016 @default.
• W2029608958 countsByYear W20296089582017 @default.
• W2029608958 countsByYear W20296089582018 @default.
• W2029608958 countsByYear W20296089582019 @default.
• W2029608958 countsByYear W20296089582020 @default.
• W2029608958 countsByYear W20296089582021 @default.
• W2029608958 countsByYear W20296089582022 @default.
• W2029608958 crossrefType "journal-article" @default.
• W2029608958 hasAuthorship W2029608958A5010921421 @default.
• W2029608958 hasAuthorship W2029608958A5019023007 @default.
• W2029608958 hasAuthorship W2029608958A5033026989 @default.
• W2029608958 hasAuthorship W2029608958A5035044300 @default.
• W2029608958 hasAuthorship W2029608958A5037124878 @default.
• W2029608958 hasAuthorship W2029608958A5052663125 @default.
• W2029608958 hasAuthorship W2029608958A5072573381 @default.
• W2029608958 hasAuthorship W2029608958A5078789225 @default.
• W2029608958 hasBestOaLocation W20296089581 @default.
• W2029608958 hasConcept C112446052 @default.
• W2029608958 hasConcept C12554922 @default.
• W2029608958 hasConcept C126322002 @default.
• W2029608958 hasConcept C134018914 @default.
• W2029608958 hasConcept C170493617 @default.
• W2029608958 hasConcept C178790620 @default.
• W2029608958 hasConcept C185592680 @default.
• W2029608958 hasConcept C198091228 @default.
• W2029608958 hasConcept C2777391703 @default.
• W2029608958 hasConcept C2779306644 @default.
• W2029608958 hasConcept C55493867 @default.
• W2029608958 hasConcept C71924100 @default.
• W2029608958 hasConcept C86803240 @default.
• W2029608958 hasConcept C95444343 @default.
• W2029608958 hasConceptScore W2029608958C112446052 @default.
• W2029608958 hasConceptScore W2029608958C12554922 @default.
• W2029608958 hasConceptScore W2029608958C126322002 @default.
• W2029608958 hasConceptScore W2029608958C134018914 @default.
• W2029608958 hasConceptScore W2029608958C170493617 @default.
• W2029608958 hasConceptScore W2029608958C178790620 @default.
• W2029608958 hasConceptScore W2029608958C185592680 @default.
• W2029608958 hasConceptScore W2029608958C198091228 @default.
• W2029608958 hasConceptScore W2029608958C2777391703 @default.
• W2029608958 hasConceptScore W2029608958C2779306644 @default.
• W2029608958 hasConceptScore W2029608958C55493867 @default.
• W2029608958 hasConceptScore W2029608958C71924100 @default.
• W2029608958 hasConceptScore W2029608958C86803240 @default.
• W2029608958 hasConceptScore W2029608958C95444343 @default.
• W2029608958 hasIssue "47" @default.
• W2029608958 hasLocation W20296089581 @default.
• W2029608958 hasLocation W20296089582 @default.
• W2029608958 hasLocation W20296089583 @default.

• next