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- W2029660411 abstract "This paper describes a novel noninvasive method to study the changes in free carbohydrates excreted in urine as a result of toxicity in the rat induced by the administration of puromycin aminonucleoside (PAN). Urine samples were collected for 24 h prior to dosing and at 8, 24, and 32 h postdosing. For each sample, free carbohydrates were extracted from the urine using a graphitized carbon column and then labeled with 2-aminoacridone (2-AMAC) prior to analysis by hydrophilic interaction liquid chromatography (HILC). Dramatic changes were seen in the profile of the carbohydrates at the 8- and 24-h time points. These changes in carbohydrate profiles may be useful as early indicators of toxicity." @default.
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- W2029660411 date "2000-08-01" @default.
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- W2029660411 title "Early Indication of Effects of Puromycin Aminonucleoside Using a Fluorimetric Assay of 2-Aminoacridone-Derivatized Carbohydrates in Urine" @default.
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- W2029660411 doi "https://doi.org/10.1006/abio.2000.4628" @default.
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