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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029674080> ?p ?o ?g. }

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• W2029674080 abstract "The purpose of this study was to determine whether retrovirus-mediated transfer of murine macrophage inducible nitric oxide synthase (iNOS) can produce inhibition of tumorigenicity and metastasis. Retroviral vectors encoding macrophage iNOS constructed in pLXSN, a retroviral vector with the iNOS gene under the control of a long terminal repeat promoter, were stably transfected into PA317 cells. Medium harvested from confluent monolayers of the virus-producing cell lines was used for infection of the murine K-1735 melanoma cells. Expression of iNOS was confirmed by northern and Western blot analyses. Functional iNOS protein expression was confirmed by bioassay of nitrite accumulation in the culture supernatant. Cells infected by a control iNOS-negative retrovirus produced fast-growing subcutaneous tumors and many lung metastases in nude mice, whereas iNOS-transduced cells produced slow-growing tumors and few lung metastases, showing that the infection of murine tumor cells by retroviruses harboring the iNOS gene can suppress tumorigenicity and metastasis." @default.
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• W2029674080 date "1997-01-01" @default.
• W2029674080 modified "2023-10-06" @default.
• W2029674080 title "Use of Retroviral Vectors Encoding Murine Inducible Nitric Oxide Synthase Gene to Suppress Tumorigenicity and Cancer Metastasis of Murine Melanoma" @default.
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• W2029674080 doi "https://doi.org/10.1089/cbr.1997.12.167" @default.
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