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- W2029688512 abstract "We studied the effects of temperature on the sulfate permease of Penicillium chrysogenum (PC) (a mesophile with a growth temperature range of 4-35°C) and Penicillium duponti (PD) (a thermophile with a growth temperature range of 27-58°C). Arrhenius plots of sulfate permease activity from mycelia grown at 50°C (PD), 30°C (PD and PC) or 8°C (PC) indicate that at temperatures below the transition point there is little difference in the activation energy of sulfate permease in mycelia from PD grown at 50°C or 30°C or PC grown at 30°C or 8°C; however, the temperature of the transition point for the permease from each set of mycelia assayed reflects the optimum growth temperature of the fungal source. Transitions occur at 15 °C for mycelia from PC and 35 ° C for PD mycelia. Kinetic measurements indicate that the Km of sulfate permease in PC cells grown at a variety of temperatures is essentially the same at various temperatures. As an example, the Km of 8°C or 30°C grown PC is about 55 μM at 25°C and 45 μM at 8°C. Vmax measurements reflect growth conditions such as temperature and growth stage. p]Lipid composition of the mycelia dramatically reflect growth temperatures. Double bond index values vary from 1.94 for PC grown at 8°C to 0.81 for PD grown at 50°C. The percentage of total fatty acid represented by linolenic acid varies from 45% in 8°C grown PC to 4.2% or less in 30°C grown PC. No linolenic is found in mycelia from PD." @default.
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- W2029688512 date "1987-09-01" @default.
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- W2029688512 title "Effect of temperature on sulfate transport in two Penicillia" @default.
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- W2029688512 doi "https://doi.org/10.1016/s0376-7388(00)80379-8" @default.
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