Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029698947> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2029698947 endingPage "1342" @default.
- W2029698947 startingPage "1338" @default.
- W2029698947 abstract "The intestinal brush-border enzyme lactase splits lactose into its component monosaccharides, glucose and galactose. Relative deficiency of the enzyme during adulthood is a common condition worldwide and is frequently associated with symptoms of lactose intolerance. We studied the synthesis and processing of lactase in normal and adult hypolactasic subjects using human intestinal explants in organ culture. Metabolic labeling experiments in our control subjects with [35S]methionine followed by immunoprecipitation, sodium dodecyl sulfate-polyacrylamide-gel electrophoresis, and fluorography demonstrated that newly synthesized lactase is initially recognized as a precursor molecule with a relative molecular weight (Mr) of 205,000. Over the course of several hours most of the labeled lactase was converted to a mature form of 150,000 Mr. Transiently appearing forms of 215,000 and 190,000 Mr were identified and were felt to represent intermediary species generated during intracellular processing. We identified two distinct alterations in lactase biosynthesis accounting for adult hypolactasia. Studies in three deficient subjects demonstrated markedly reduced synthesis of the precursor protein though posttranslational processing appeared identical to normal. Multiple studies in a fourth deficient subject demonstrated synthesis of ample amounts of precursor lactase but reduced conversion to the mature active form of the enzyme." @default.
- W2029698947 created "2016-06-24" @default.
- W2029698947 creator A5007414268 @default.
- W2029698947 creator A5030800081 @default.
- W2029698947 creator A5065269608 @default.
- W2029698947 creator A5082421743 @default.
- W2029698947 creator A5089281470 @default.
- W2029698947 date "1990-10-01" @default.
- W2029698947 modified "2023-10-15" @default.
- W2029698947 title "The biosynthetic basis of adult lactase deficiency." @default.
- W2029698947 cites W1040845057 @default.
- W2029698947 cites W1560300183 @default.
- W2029698947 cites W1765133524 @default.
- W2029698947 cites W1970791631 @default.
- W2029698947 cites W200081977 @default.
- W2029698947 cites W2018289835 @default.
- W2029698947 cites W2058669904 @default.
- W2029698947 cites W2060459207 @default.
- W2029698947 cites W2067408128 @default.
- W2029698947 cites W2068690187 @default.
- W2029698947 cites W2100837269 @default.
- W2029698947 cites W2110389243 @default.
- W2029698947 cites W2341803145 @default.
- W2029698947 cites W2412424882 @default.
- W2029698947 cites W7386683 @default.
- W2029698947 doi "https://doi.org/10.1172/jci114843" @default.
- W2029698947 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/296867" @default.
- W2029698947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2120287" @default.
- W2029698947 hasPublicationYear "1990" @default.
- W2029698947 type Work @default.
- W2029698947 sameAs 2029698947 @default.
- W2029698947 citedByCount "54" @default.
- W2029698947 countsByYear W20296989472015 @default.
- W2029698947 countsByYear W20296989472021 @default.
- W2029698947 countsByYear W20296989472022 @default.
- W2029698947 crossrefType "journal-article" @default.
- W2029698947 hasAuthorship W2029698947A5007414268 @default.
- W2029698947 hasAuthorship W2029698947A5030800081 @default.
- W2029698947 hasAuthorship W2029698947A5065269608 @default.
- W2029698947 hasAuthorship W2029698947A5082421743 @default.
- W2029698947 hasAuthorship W2029698947A5089281470 @default.
- W2029698947 hasBestOaLocation W20296989471 @default.
- W2029698947 hasConcept C116084860 @default.
- W2029698947 hasConcept C126322002 @default.
- W2029698947 hasConcept C130316041 @default.
- W2029698947 hasConcept C134018914 @default.
- W2029698947 hasConcept C181199279 @default.
- W2029698947 hasConcept C2778173381 @default.
- W2029698947 hasConcept C2778724459 @default.
- W2029698947 hasConcept C2778974006 @default.
- W2029698947 hasConcept C2780912031 @default.
- W2029698947 hasConcept C41625074 @default.
- W2029698947 hasConcept C515207424 @default.
- W2029698947 hasConcept C5426402 @default.
- W2029698947 hasConcept C553450214 @default.
- W2029698947 hasConcept C55493867 @default.
- W2029698947 hasConcept C71924100 @default.
- W2029698947 hasConcept C86803240 @default.
- W2029698947 hasConceptScore W2029698947C116084860 @default.
- W2029698947 hasConceptScore W2029698947C126322002 @default.
- W2029698947 hasConceptScore W2029698947C130316041 @default.
- W2029698947 hasConceptScore W2029698947C134018914 @default.
- W2029698947 hasConceptScore W2029698947C181199279 @default.
- W2029698947 hasConceptScore W2029698947C2778173381 @default.
- W2029698947 hasConceptScore W2029698947C2778724459 @default.
- W2029698947 hasConceptScore W2029698947C2778974006 @default.
- W2029698947 hasConceptScore W2029698947C2780912031 @default.
- W2029698947 hasConceptScore W2029698947C41625074 @default.
- W2029698947 hasConceptScore W2029698947C515207424 @default.
- W2029698947 hasConceptScore W2029698947C5426402 @default.
- W2029698947 hasConceptScore W2029698947C553450214 @default.
- W2029698947 hasConceptScore W2029698947C55493867 @default.
- W2029698947 hasConceptScore W2029698947C71924100 @default.
- W2029698947 hasConceptScore W2029698947C86803240 @default.
- W2029698947 hasIssue "4" @default.
- W2029698947 hasLocation W20296989471 @default.
- W2029698947 hasLocation W20296989472 @default.
- W2029698947 hasLocation W20296989473 @default.
- W2029698947 hasLocation W20296989474 @default.
- W2029698947 hasOpenAccess W2029698947 @default.
- W2029698947 hasPrimaryLocation W20296989471 @default.
- W2029698947 hasRelatedWork W1531437899 @default.
- W2029698947 hasRelatedWork W1979657473 @default.
- W2029698947 hasRelatedWork W2008675487 @default.
- W2029698947 hasRelatedWork W2183327365 @default.
- W2029698947 hasRelatedWork W2357926609 @default.
- W2029698947 hasRelatedWork W2365096461 @default.
- W2029698947 hasRelatedWork W2382874577 @default.
- W2029698947 hasRelatedWork W2908239555 @default.
- W2029698947 hasRelatedWork W2964767015 @default.
- W2029698947 hasRelatedWork W82481476 @default.
- W2029698947 hasVolume "86" @default.
- W2029698947 isParatext "false" @default.
- W2029698947 isRetracted "false" @default.
- W2029698947 magId "2029698947" @default.
- W2029698947 workType "article" @default.