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- W2029703020 abstract "The role of adrenocortical steroids in distal nephron acidification was studied in rats by measuring urine minus blood [Formula: see text] differences (U – B [Formula: see text]) in control, sham-operated, and adrenalectomized (ADX) animals. Operations were performed 48 h before experiments. During the experiments, all rats received an infusion of 0.35–0.60 M NaHCO 3 , leading to urine bicarbonate concentrations in the order of 100–200 mM. Adrenalectomized rats had significantly decreased U – B [Formula: see text] (11.9 ± 1.99 mmHg; 1 mmHg = 133.3 Pa) with respect to sham-operated rats (39.9 ± 1.26 mmHg). In another series, ADX rats received supplements of the adrenal steroids corticosterone, aldosterone, and 18-hydroxycorticosterone 100 min before the experiment. U – B [Formula: see text] increased after hormone administration: corticosterone, 30.0 ± 2.13 mmHg; aldosterone, 26.6 ± 1.74 mmHg; 18-hydroxycorticosterone, 29.0 ± 1.60 mmHg; but none restored these values to normal. Combinations of two hormones were also used; only aldosterone + corticosterone restored U – B [Formula: see text] to normal: 39.0 ± 1.66 mmHg. Renal phosphate excretion (but not urine phosphate levels) decreased significantly in ADX as compared with sham-operated rats. Extracellular volume was not significantly affected in ADX rats, which received ad libitum 0.9% NaCl for drinking. It is concluded that distal tubular acidification, as evaluated by U – B [Formula: see text], is dependent on cortical steroids.Key words: urine minus blood [Formula: see text], urine acidification, distal nephron, adrenal steroids." @default.
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- W2029703020 title "Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification" @default.
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- W2029703020 doi "https://doi.org/10.1139/y89-097" @default.
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