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• W2029747879 abstract "The present study was designed to investigate the phenotypic transformation and migration of adventitial fibroblasts using 5-bromo-2'-deoxyuridine (BrdU) labeling following angioplasty and to explore the correlation between adventitial cells and post-angioplasty restenosis. A vascular restenosis model was established in 23 rats by injuring the common carotid artery with a wire. BrDU was used to label the fibroblasts followed by immunohistochemistry for α-actin. Blood vessels were observed under light microscopy and scanning electron microscopy followed by image analysis. The number of BrDU-positive fibroblasts in the intima, media and adventitia of the blood vessels was determined 3, 7, 41 and 28 days after injury. The results demonstrated that at different time points, the number of BrDU-positive cells was significantly different in the intima, media and adventia (P<0.05). Electron microscopy indicated that the fibroblasts were full of cytoplasm. In addition, many secretory granules were noted on the rough endoplasmic reticulum and a large amount of microfilament bundles were noted after angioplasty. The fibroblasts transformed into myofibroblasts. Seven and 14 days after injury, the myofibroblasts formed wide pseudopods stretching to the fenestrae of the external and internal elastic lamina, and cells had a tendency to migrate into the lumen. The fibroblasts in the adventitia underwent transformation after percutaneous transluminal angioplasty and secreted α-actin. In conclusion, the fibroblasts in the adventitia transformed into myofibroblasts, migrated into and proliferated in the intima and became a component of the newly generated intima. Adventitial cells are thus related to vascular restenosis." @default.
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• W2029747879 date "2012-04-17" @default.
• W2029747879 modified "2023-10-01" @default.
• W2029747879 title "Phenotypic transformation and migration of adventitial cells following angioplasty" @default.
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• W2029747879 doi "https://doi.org/10.3892/etm.2012.551" @default.
• W2029747879 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3460273" @default.
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