Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029753514> ?p ?o ?g. }
- W2029753514 endingPage "147" @default.
- W2029753514 startingPage "121" @default.
- W2029753514 abstract "1. Whole-cell potassium currents of freshly isolated human (adult and fetal) and monkey (adult) retinal pigment epithelial (RPE) cells, as well as cultured human and monkey RPE cells were studied using the patch-clamp technique. 2. In freshly isolated adult cells of both species, two currents were observed in the voltage range from -150 to +50 mV: an outwardly rectifying current and an inwardly rectifying current. These currents were also found in cultured cells of both species. 3. The outwardly rectifying current in freshly isolated adult human and monkey cells and some cultured cells was evoked by depolarizing voltage pulses more positive that -30 mV. The current activated with a sigmoidal time course after a brief delay, and was virtually non-inactivating. The conductance associated with the current was half-maximal at -16.4 mV for fresh human cells and -13.5 mV for fresh monkey cells, but was shifted 16.0 and 17.7 mV in the positive direction in cultured human and monkey cells, respectively. The reversal potential of the current in both human and monkey cells matched the potassium equilibrium potential (EK) over a wide range of external potassium concentrations. This current was blocked by 20 mM tetraethylammonium. 4. A membrane current that exhibited inward rectification was observed with hyperpolarizing voltage pulses. The zero-current potential of this current was close to EK. This current was blocked by 2 mM Ba2+ and 2 mM Cs+. In cultured human and monkey cells, but not in fresh cells, this current exhibited an inactivation when voltage pulses were more negative than -120 mV. External Na+ was responsible for the inactivation, as the inactivation was removed in a Na(+)-free solution. 5. Membrane currents in freshly isolated fetal human RPE cells were remarkably different from those in adult cells. A transient outward current resembling the A-type potassium current was observed as the dominant membrane current in freshly isolated fetal human cells. This current activated when voltage pulses were more positive than -30 mV. It inactivated rapidly after reaching a maximal level. Application of 5 mM 4-aminopyridine (4-AP) completely blocked this current. Although this current was never observed in fresh adult cells, it was found in 33% of the cultured adult cells with similar kinetics, ion selectivity, and pharmacological properties. 6. In about 26% of the freshly isolated fetal human cells, a more slowly activating outward current, which resembled the delayed rectifier, was found to co-exist with the transient outward current.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
- W2029753514 created "2016-06-24" @default.
- W2029753514 creator A5006431062 @default.
- W2029753514 creator A5020489484 @default.
- W2029753514 creator A5042314019 @default.
- W2029753514 date "1993-06-01" @default.
- W2029753514 modified "2023-10-16" @default.
- W2029753514 title "Whole-cell K+ currents in fresh and cultured cells of the human and monkey retinal pigment epithelium." @default.
- W2029753514 cites W114316392 @default.
- W2029753514 cites W1480979853 @default.
- W2029753514 cites W1542171240 @default.
- W2029753514 cites W1970930847 @default.
- W2029753514 cites W1971655733 @default.
- W2029753514 cites W1972460372 @default.
- W2029753514 cites W1978252033 @default.
- W2029753514 cites W1979005514 @default.
- W2029753514 cites W1985940938 @default.
- W2029753514 cites W1992680491 @default.
- W2029753514 cites W1994463799 @default.
- W2029753514 cites W1996062006 @default.
- W2029753514 cites W1997511216 @default.
- W2029753514 cites W2000306425 @default.
- W2029753514 cites W2004947583 @default.
- W2029753514 cites W2009667219 @default.
- W2029753514 cites W2009854441 @default.
- W2029753514 cites W2013278153 @default.
- W2029753514 cites W2015426350 @default.
- W2029753514 cites W2021564846 @default.
- W2029753514 cites W2035804123 @default.
- W2029753514 cites W2062963816 @default.
- W2029753514 cites W2068298862 @default.
- W2029753514 cites W2075781211 @default.
- W2029753514 cites W2083118578 @default.
- W2029753514 cites W2084531756 @default.
- W2029753514 cites W2084873082 @default.
- W2029753514 cites W2092745209 @default.
- W2029753514 cites W2094882917 @default.
- W2029753514 cites W2118397392 @default.
- W2029753514 cites W2154917327 @default.
- W2029753514 cites W2344841325 @default.
- W2029753514 cites W4238401914 @default.
- W2029753514 doi "https://doi.org/10.1113/jphysiol.1993.sp019669" @default.
- W2029753514 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1175422" @default.
- W2029753514 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8229830" @default.
- W2029753514 hasPublicationYear "1993" @default.
- W2029753514 type Work @default.
- W2029753514 sameAs 2029753514 @default.
- W2029753514 citedByCount "56" @default.
- W2029753514 countsByYear W20297535142012 @default.
- W2029753514 countsByYear W20297535142013 @default.
- W2029753514 countsByYear W20297535142014 @default.
- W2029753514 countsByYear W20297535142020 @default.
- W2029753514 countsByYear W20297535142021 @default.
- W2029753514 countsByYear W20297535142022 @default.
- W2029753514 countsByYear W20297535142023 @default.
- W2029753514 crossrefType "journal-article" @default.
- W2029753514 hasAuthorship W2029753514A5006431062 @default.
- W2029753514 hasAuthorship W2029753514A5020489484 @default.
- W2029753514 hasAuthorship W2029753514A5042314019 @default.
- W2029753514 hasBestOaLocation W20297535141 @default.
- W2029753514 hasConcept C100837961 @default.
- W2029753514 hasConcept C12554922 @default.
- W2029753514 hasConcept C147944092 @default.
- W2029753514 hasConcept C169760540 @default.
- W2029753514 hasConcept C178790620 @default.
- W2029753514 hasConcept C181911157 @default.
- W2029753514 hasConcept C185263204 @default.
- W2029753514 hasConcept C185592680 @default.
- W2029753514 hasConcept C2778024200 @default.
- W2029753514 hasConcept C2778071365 @default.
- W2029753514 hasConcept C2780827179 @default.
- W2029753514 hasConcept C2781295685 @default.
- W2029753514 hasConcept C4141045 @default.
- W2029753514 hasConcept C517785266 @default.
- W2029753514 hasConcept C54355233 @default.
- W2029753514 hasConcept C55493867 @default.
- W2029753514 hasConcept C81885089 @default.
- W2029753514 hasConcept C83743174 @default.
- W2029753514 hasConcept C86803240 @default.
- W2029753514 hasConcept C95444343 @default.
- W2029753514 hasConceptScore W2029753514C100837961 @default.
- W2029753514 hasConceptScore W2029753514C12554922 @default.
- W2029753514 hasConceptScore W2029753514C147944092 @default.
- W2029753514 hasConceptScore W2029753514C169760540 @default.
- W2029753514 hasConceptScore W2029753514C178790620 @default.
- W2029753514 hasConceptScore W2029753514C181911157 @default.
- W2029753514 hasConceptScore W2029753514C185263204 @default.
- W2029753514 hasConceptScore W2029753514C185592680 @default.
- W2029753514 hasConceptScore W2029753514C2778024200 @default.
- W2029753514 hasConceptScore W2029753514C2778071365 @default.
- W2029753514 hasConceptScore W2029753514C2780827179 @default.
- W2029753514 hasConceptScore W2029753514C2781295685 @default.
- W2029753514 hasConceptScore W2029753514C4141045 @default.
- W2029753514 hasConceptScore W2029753514C517785266 @default.
- W2029753514 hasConceptScore W2029753514C54355233 @default.
- W2029753514 hasConceptScore W2029753514C55493867 @default.
- W2029753514 hasConceptScore W2029753514C81885089 @default.
- W2029753514 hasConceptScore W2029753514C83743174 @default.