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- W2029789966 abstract "The number of importance of infections caused by Mycobacterium tuberculosis and other mycobacterial species is increasing. Since the detection and identification of mycobacteria by conventional laboratory methods (cultivation, staining, and biochemical tests) is a slow and complex procedure, rapid diagnostic methods are urgently needed. Several amplification methods based on different techniques have been applied in the detection of mycobacteria directly from clinical specimens. Most experience has been obtained from different polymerase chain reaction (PCR) assays and their general performance is good. However, their sensitivity in the analysis of samples containing small amounts of mycobacteria or samples containing inhibitory substances has been low. Furthermore, the risk of false positives caused by contamination is high, and the clinical relevance of the results may be unclear. Thus, these gene amplification techniques are a valuable adjunct to the diagnosis of mycobacteria, but so far they cannot replace conventional microbiological methods." @default.
- W2029789966 created "2016-06-24" @default.
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- W2029789966 date "1997-01-01" @default.
- W2029789966 modified "2023-10-16" @default.
- W2029789966 title "Gene amplification in the diagnosis of mycobacterial infections" @default.
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- W2029789966 doi "https://doi.org/10.1111/j.1699-0463.1997.tb00580.x" @default.
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