FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029807791> ?p ?o ?g. }

• W2029807791 endingPage "654" @default.
• W2029807791 startingPage "652" @default.
• W2029807791 abstract "A new epoxide hydrolase with high enantioselectivity toward (R)-glycidyl phenyl ether (R-GPE) was partially purified from Bacillus megaterium strain ECU1001. The maximum activity of the isolated enzyme was observed at 30 degrees C and pH 6.5 in a buffer system with 5% (v/v) of DMSO as a cosolvent. The enzyme was quite stable at pH 7.5 and retained full activity after incubation at 40 degrees C for 6 h. Interestingly, when the cosolvent DMSO was replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate, the apparent activity of the epoxide hydrolase significantly increased by about 1.8-fold, while the temperature optimum shifted from 30 to 40 degrees C and the half-life of the enzyme at 50 degrees C increased by 2.5 times. The enzymatic hydrolysis of rac-GPE was highly enantioselective, with an E-value (enantiomeric ratio) of 69.3 in the Tween-80 emulsion system, which is obviously higher than that (41.2) observed in the DMSO-containing system." @default.
• W2029807791 created "2016-06-24" @default.
• W2029807791 creator A5032392381 @default.
• W2029807791 creator A5068659985 @default.
• W2029807791 creator A5076286570 @default.
• W2029807791 creator A5078041205 @default.
• W2029807791 date "2003-01-01" @default.
• W2029807791 modified "2023-10-15" @default.
• W2029807791 title "Improved Catalytic Performance of Bacillus megaterium Epoxide Hydrolase in a Medium Containing Tween-80" @default.
• W2029807791 cites W125551066 @default.
• W2029807791 cites W1964426632 @default.
• W2029807791 cites W1968901653 @default.
• W2029807791 cites W1972979010 @default.
• W2029807791 cites W1979922397 @default.
• W2029807791 cites W2026009003 @default.
• W2029807791 cites W2026117532 @default.
• W2029807791 cites W2056922869 @default.
• W2029807791 cites W2063946543 @default.
• W2029807791 cites W2081270195 @default.
• W2029807791 cites W2093006140 @default.
• W2029807791 cites W4293247451 @default.
• W2029807791 doi "https://doi.org/10.1021/bp020293v" @default.
• W2029807791 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12675611" @default.
• W2029807791 hasPublicationYear "2003" @default.
• W2029807791 type Work @default.
• W2029807791 sameAs 2029807791 @default.
• W2029807791 citedByCount "21" @default.
• W2029807791 countsByYear W20298077912012 @default.
• W2029807791 countsByYear W20298077912013 @default.
• W2029807791 countsByYear W20298077912014 @default.
• W2029807791 countsByYear W20298077912015 @default.
• W2029807791 countsByYear W20298077912017 @default.
• W2029807791 countsByYear W20298077912018 @default.
• W2029807791 crossrefType "journal-article" @default.
• W2029807791 hasAuthorship W2029807791A5032392381 @default.
• W2029807791 hasAuthorship W2029807791A5068659985 @default.
• W2029807791 hasAuthorship W2029807791A5076286570 @default.
• W2029807791 hasAuthorship W2029807791A5078041205 @default.
• W2029807791 hasConcept C101819947 @default.
• W2029807791 hasConcept C146686406 @default.
• W2029807791 hasConcept C161790260 @default.
• W2029807791 hasConcept C178790620 @default.
• W2029807791 hasConcept C181199279 @default.
• W2029807791 hasConcept C185592680 @default.
• W2029807791 hasConcept C18903297 @default.
• W2029807791 hasConcept C2776920199 @default.
• W2029807791 hasConcept C2777289219 @default.
• W2029807791 hasConcept C2778123984 @default.
• W2029807791 hasConcept C2780120296 @default.
• W2029807791 hasConcept C2780989563 @default.
• W2029807791 hasConcept C3141732 @default.
• W2029807791 hasConcept C43617362 @default.
• W2029807791 hasConcept C486523 @default.
• W2029807791 hasConcept C523546767 @default.
• W2029807791 hasConcept C54355233 @default.
• W2029807791 hasConcept C71240020 @default.
• W2029807791 hasConcept C86803240 @default.
• W2029807791 hasConcept C87644729 @default.
• W2029807791 hasConcept C94412978 @default.
• W2029807791 hasConceptScore W2029807791C101819947 @default.
• W2029807791 hasConceptScore W2029807791C146686406 @default.
• W2029807791 hasConceptScore W2029807791C161790260 @default.
• W2029807791 hasConceptScore W2029807791C178790620 @default.
• W2029807791 hasConceptScore W2029807791C181199279 @default.
• W2029807791 hasConceptScore W2029807791C185592680 @default.
• W2029807791 hasConceptScore W2029807791C18903297 @default.
• W2029807791 hasConceptScore W2029807791C2776920199 @default.
• W2029807791 hasConceptScore W2029807791C2777289219 @default.
• W2029807791 hasConceptScore W2029807791C2778123984 @default.
• W2029807791 hasConceptScore W2029807791C2780120296 @default.
• W2029807791 hasConceptScore W2029807791C2780989563 @default.
• W2029807791 hasConceptScore W2029807791C3141732 @default.
• W2029807791 hasConceptScore W2029807791C43617362 @default.
• W2029807791 hasConceptScore W2029807791C486523 @default.
• W2029807791 hasConceptScore W2029807791C523546767 @default.
• W2029807791 hasConceptScore W2029807791C54355233 @default.
• W2029807791 hasConceptScore W2029807791C71240020 @default.
• W2029807791 hasConceptScore W2029807791C86803240 @default.
• W2029807791 hasConceptScore W2029807791C87644729 @default.
• W2029807791 hasConceptScore W2029807791C94412978 @default.
• W2029807791 hasIssue "2" @default.
• W2029807791 hasLocation W20298077911 @default.
• W2029807791 hasLocation W20298077912 @default.
• W2029807791 hasOpenAccess W2029807791 @default.
• W2029807791 hasPrimaryLocation W20298077911 @default.
• W2029807791 hasRelatedWork W1974201944 @default.
• W2029807791 hasRelatedWork W1996131218 @default.
• W2029807791 hasRelatedWork W2068858913 @default.
• W2029807791 hasRelatedWork W2106529020 @default.
• W2029807791 hasRelatedWork W2112257430 @default.
• W2029807791 hasRelatedWork W2335987103 @default.
• W2029807791 hasRelatedWork W2375162076 @default.
• W2029807791 hasRelatedWork W3091912534 @default.
• W2029807791 hasRelatedWork W3146534527 @default.
• W2029807791 hasRelatedWork W4232140751 @default.
• W2029807791 hasVolume "19" @default.
• W2029807791 isParatext "false" @default.
• W2029807791 isRetracted "false" @default.

• next