FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029831722> ?p ?o ?g. }

• W2029831722 endingPage "92" @default.
• W2029831722 startingPage "84" @default.
• W2029831722 abstract "<b><i>Background:</i></b> Viral infection can exacerbate asthma by inducing the accumulation of inflammatory cells in the airway. We have previously reported that double-stranded RNA (dsRNA), a viral product and ligand of the Toll-like receptor-3 (TLR3), activates the transcription factors NF-&#954;B and IRF-3 and upregulates the expression of inflammatory chemokines in airway epithelial cells. Here, we examined the effects of the glucocorticoid fluticasone propionate (FP) on the expression of the inflammatory chemokines CCL5, CXCL8 and CXCL10. <b><i>Methods:</i></b> The airway epithelial cell line BEAS-2B was used for this study. Expression of CCL5, CXCL8 and CXCL10 mRNA and protein was quantified by real-time PCR and ELISA assay, respectively. To examine the association of FP with the physiology of chemokine production, we included several methods. Nuclear translocation of transcription factors was determined by performing Western blot analysis. Histone deacetylase (HDAC) activity in nuclear extracts was measured using a colorimetric assay. Stability of the chemokine mRNAs was examined in cells incubated with actinomycin D. The activities of the CCL5 promoter and the transcription factors NF-&#954;B and IRF-3 were assessed using luciferase reporter assays. <b><i>Results:</i></b> Treatment of BEAS-2B cells with FP significantly and dose-dependently (10^-9 to 10^-6<smlcap>M</smlcap>) inhibited dsRNA-induced expression of CCL5, CXCL8 and CXCL10 protein and mRNA, but did not affect mRNA stability. FP also significantly inhibited dsRNA-stimulated CCL5 promoter activity. However, FP had no effect on the activity of HDAC or the nuclear translocation of NF-&#954;B and IRF-3. <b><i>Conclusions:</i></b> FP inhibits the dsRNA-stimulated expression of inflammatory chemokines in airway epithelial cells. FP may act by inhibiting chemokine transcription through an as yet unidentified mechanism." @default.
• W2029831722 created "2016-06-24" @default.
• W2029831722 creator A5011460730 @default.
• W2029831722 creator A5016377115 @default.
• W2029831722 creator A5022154349 @default.
• W2029831722 creator A5031036106 @default.
• W2029831722 creator A5038689896 @default.
• W2029831722 creator A5056666751 @default.
• W2029831722 creator A5067631088 @default.
• W2029831722 creator A5074633922 @default.
• W2029831722 creator A5076009034 @default.
• W2029831722 creator A5077023340 @default.
• W2029831722 creator A5083475604 @default.
• W2029831722 date "2013-01-01" @default.
• W2029831722 modified "2023-09-27" @default.
• W2029831722 title "Basic Research on Virus-Induced Asthma Exacerbation: Inhibition of Inflammatory Chemokine Expression by Fluticasone Propionate" @default.
• W2029831722 cites W1605768991 @default.
• W2029831722 cites W1820270771 @default.
• W2029831722 cites W1981703255 @default.
• W2029831722 cites W1985083242 @default.
• W2029831722 cites W1993296518 @default.
• W2029831722 cites W1999005213 @default.
• W2029831722 cites W1999439773 @default.
• W2029831722 cites W2005850712 @default.
• W2029831722 cites W2007441206 @default.
• W2029831722 cites W2034215339 @default.
• W2029831722 cites W2057704272 @default.
• W2029831722 cites W2065445260 @default.
• W2029831722 cites W2079139077 @default.
• W2029831722 cites W2097063436 @default.
• W2029831722 cites W2099929996 @default.
• W2029831722 cites W2116208806 @default.
• W2029831722 cites W2121034406 @default.
• W2029831722 cites W2121268449 @default.
• W2029831722 cites W2140958544 @default.
• W2029831722 cites W2160971027 @default.
• W2029831722 cites W2164610956 @default.
• W2029831722 doi "https://doi.org/10.1159/000350455" @default.
• W2029831722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4841689" @default.
• W2029831722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23711858" @default.
• W2029831722 hasPublicationYear "2013" @default.
• W2029831722 type Work @default.
• W2029831722 sameAs 2029831722 @default.
• W2029831722 citedByCount "17" @default.
• W2029831722 countsByYear W20298317222014 @default.
• W2029831722 countsByYear W20298317222015 @default.
• W2029831722 countsByYear W20298317222016 @default.
• W2029831722 countsByYear W20298317222018 @default.
• W2029831722 countsByYear W20298317222020 @default.
• W2029831722 countsByYear W20298317222021 @default.
• W2029831722 countsByYear W20298317222022 @default.
• W2029831722 crossrefType "journal-article" @default.
• W2029831722 hasAuthorship W2029831722A5011460730 @default.
• W2029831722 hasAuthorship W2029831722A5016377115 @default.
• W2029831722 hasAuthorship W2029831722A5022154349 @default.
• W2029831722 hasAuthorship W2029831722A5031036106 @default.
• W2029831722 hasAuthorship W2029831722A5038689896 @default.
• W2029831722 hasAuthorship W2029831722A5056666751 @default.
• W2029831722 hasAuthorship W2029831722A5067631088 @default.
• W2029831722 hasAuthorship W2029831722A5074633922 @default.
• W2029831722 hasAuthorship W2029831722A5076009034 @default.
• W2029831722 hasAuthorship W2029831722A5077023340 @default.
• W2029831722 hasAuthorship W2029831722A5083475604 @default.
• W2029831722 hasBestOaLocation W20298317222 @default.
• W2029831722 hasConcept C116484652 @default.
• W2029831722 hasConcept C13373296 @default.
• W2029831722 hasConcept C153911025 @default.
• W2029831722 hasConcept C185592680 @default.
• W2029831722 hasConcept C203014093 @default.
• W2029831722 hasConcept C2776090121 @default.
• W2029831722 hasConcept C2776914184 @default.
• W2029831722 hasConcept C5858377 @default.
• W2029831722 hasConcept C68324877 @default.
• W2029831722 hasConcept C79484868 @default.
• W2029831722 hasConcept C86803240 @default.
• W2029831722 hasConcept C8891405 @default.
• W2029831722 hasConceptScore W2029831722C116484652 @default.
• W2029831722 hasConceptScore W2029831722C13373296 @default.
• W2029831722 hasConceptScore W2029831722C153911025 @default.
• W2029831722 hasConceptScore W2029831722C185592680 @default.
• W2029831722 hasConceptScore W2029831722C203014093 @default.
• W2029831722 hasConceptScore W2029831722C2776090121 @default.
• W2029831722 hasConceptScore W2029831722C2776914184 @default.
• W2029831722 hasConceptScore W2029831722C5858377 @default.
• W2029831722 hasConceptScore W2029831722C68324877 @default.
• W2029831722 hasConceptScore W2029831722C79484868 @default.
• W2029831722 hasConceptScore W2029831722C86803240 @default.
• W2029831722 hasConceptScore W2029831722C8891405 @default.
• W2029831722 hasIssue "Suppl. 2" @default.
• W2029831722 hasLocation W20298317221 @default.
• W2029831722 hasLocation W20298317222 @default.
• W2029831722 hasLocation W20298317223 @default.
• W2029831722 hasLocation W20298317224 @default.
• W2029831722 hasOpenAccess W2029831722 @default.
• W2029831722 hasPrimaryLocation W20298317221 @default.
• W2029831722 hasRelatedWork W112705378 @default.
• W2029831722 hasRelatedWork W1538511001 @default.
• W2029831722 hasRelatedWork W2070463916 @default.

• next