FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029861730> ?p ?o ?g. }

• W2029861730 endingPage "320" @default.
• W2029861730 startingPage "314" @default.
• W2029861730 abstract "In this work, a specific, sensitive, and accurate technique was presented for simultaneous detection of Salmonella spp., Shigella spp., and Staphylococcus aureus in food products, three of the more frequent foodborne pathogens that were usually reported in a variety of food matrices. An internal amplification control (IAC) was added in a multiplex PCR (mPCR) reaction system as an indicator of false negative result that can come from the presence of PCR inhibitors in food products. In the presence of inhibitor, no signal would result for the target genes as well as the IAC which results in a positive signal, thereby, eliminating false negative results. To ensure detection of only the viable cells, the effects of sodium deoxycholate (SD) in combination with propidium monoazide (PMA) treatment in the presence of dead cells and viable cells were investigated. Results showed that PMA treatment alone could not effectively inhibit the detection of 107 CFU/mL of dead Salmonella Typhimurium, Shigella sonnei, and S. aureus from PCR amplification. However, the SD in combination with PMA treatment gave negative results for PCR amplification of dead S. Typhimurium, S. sonnei, and S. aureus in pure culture and food products. When the developed SD-PMA-mPCR assay in combination with IAC was applied to detect the spiked food (milk, ground beef), the LOD of SD-PMA-mPCR assay for S. Typhimurium, S. sonnei, and S. aureus inoculated individually or inoculated simultaneously into milk or ground beef were 101 CFU/mL or 101 CFU/g after 15 h enrichment. The results suggested that the SD-PMA-mPCR assay in combination with IAC held promise for the detection of foodborne S. Typhimurium, S. sonnei, and S. aureus." @default.
• W2029861730 created "2016-06-24" @default.
• W2029861730 creator A5000815865 @default.
• W2029861730 creator A5015099801 @default.
• W2029861730 creator A5031615088 @default.
• W2029861730 creator A5038757746 @default.
• W2029861730 creator A5064140180 @default.
• W2029861730 creator A5075676603 @default.
• W2029861730 creator A5078793883 @default.
• W2029861730 date "2015-11-01" @default.
• W2029861730 modified "2023-10-17" @default.
• W2029861730 title "Development of an SD-PMA-mPCR assay with internal amplification control for rapid and sensitive detection of viable Salmonella spp., Shigella spp. and Staphylococcus aureus in food products" @default.
• W2029861730 cites W1518264471 @default.
• W2029861730 cites W1525752846 @default.
• W2029861730 cites W1804759123 @default.
• W2029861730 cites W1966642304 @default.
• W2029861730 cites W1971377917 @default.
• W2029861730 cites W1975793761 @default.
• W2029861730 cites W1976836197 @default.
• W2029861730 cites W1978923651 @default.
• W2029861730 cites W1986519740 @default.
• W2029861730 cites W2000826316 @default.
• W2029861730 cites W2010229814 @default.
• W2029861730 cites W2017409062 @default.
• W2029861730 cites W2019743688 @default.
• W2029861730 cites W2047294242 @default.
• W2029861730 cites W2054058229 @default.
• W2029861730 cites W2058635744 @default.
• W2029861730 cites W2065842063 @default.
• W2029861730 cites W2075240099 @default.
• W2029861730 cites W2089306901 @default.
• W2029861730 cites W2091849359 @default.
• W2029861730 cites W2095989309 @default.
• W2029861730 cites W2142476637 @default.
• W2029861730 doi "https://doi.org/10.1016/j.foodcont.2015.04.016" @default.
• W2029861730 hasPublicationYear "2015" @default.
• W2029861730 type Work @default.
• W2029861730 sameAs 2029861730 @default.
• W2029861730 citedByCount "31" @default.
• W2029861730 countsByYear W20298617302016 @default.
• W2029861730 countsByYear W20298617302017 @default.
• W2029861730 countsByYear W20298617302018 @default.
• W2029861730 countsByYear W20298617302019 @default.
• W2029861730 countsByYear W20298617302020 @default.
• W2029861730 countsByYear W20298617302021 @default.
• W2029861730 countsByYear W20298617302022 @default.
• W2029861730 countsByYear W20298617302023 @default.
• W2029861730 crossrefType "journal-article" @default.
• W2029861730 hasAuthorship W2029861730A5000815865 @default.
• W2029861730 hasAuthorship W2029861730A5015099801 @default.
• W2029861730 hasAuthorship W2029861730A5031615088 @default.
• W2029861730 hasAuthorship W2029861730A5038757746 @default.
• W2029861730 hasAuthorship W2029861730A5064140180 @default.
• W2029861730 hasAuthorship W2029861730A5075676603 @default.
• W2029861730 hasAuthorship W2029861730A5078793883 @default.
• W2029861730 hasConcept C104317684 @default.
• W2029861730 hasConcept C185592680 @default.
• W2029861730 hasConcept C2776958425 @default.
• W2029861730 hasConcept C2776986154 @default.
• W2029861730 hasConcept C2779489039 @default.
• W2029861730 hasConcept C2781065037 @default.
• W2029861730 hasConcept C2911111840 @default.
• W2029861730 hasConcept C31903555 @default.
• W2029861730 hasConcept C49105822 @default.
• W2029861730 hasConcept C523546767 @default.
• W2029861730 hasConcept C54355233 @default.
• W2029861730 hasConcept C55493867 @default.
• W2029861730 hasConcept C86803240 @default.
• W2029861730 hasConcept C89423630 @default.
• W2029861730 hasConceptScore W2029861730C104317684 @default.
• W2029861730 hasConceptScore W2029861730C185592680 @default.
• W2029861730 hasConceptScore W2029861730C2776958425 @default.
• W2029861730 hasConceptScore W2029861730C2776986154 @default.
• W2029861730 hasConceptScore W2029861730C2779489039 @default.
• W2029861730 hasConceptScore W2029861730C2781065037 @default.
• W2029861730 hasConceptScore W2029861730C2911111840 @default.
• W2029861730 hasConceptScore W2029861730C31903555 @default.
• W2029861730 hasConceptScore W2029861730C49105822 @default.
• W2029861730 hasConceptScore W2029861730C523546767 @default.
• W2029861730 hasConceptScore W2029861730C54355233 @default.
• W2029861730 hasConceptScore W2029861730C55493867 @default.
• W2029861730 hasConceptScore W2029861730C86803240 @default.
• W2029861730 hasConceptScore W2029861730C89423630 @default.
• W2029861730 hasFunder F4320326881 @default.
• W2029861730 hasLocation W20298617301 @default.
• W2029861730 hasOpenAccess W2029861730 @default.
• W2029861730 hasPrimaryLocation W20298617301 @default.
• W2029861730 hasRelatedWork W1865807250 @default.
• W2029861730 hasRelatedWork W1994452894 @default.
• W2029861730 hasRelatedWork W2029861730 @default.
• W2029861730 hasRelatedWork W2038580899 @default.
• W2029861730 hasRelatedWork W2047468568 @default.
• W2029861730 hasRelatedWork W2352234175 @default.
• W2029861730 hasRelatedWork W2389599546 @default.
• W2029861730 hasRelatedWork W2412729527 @default.
• W2029861730 hasRelatedWork W272264176 @default.
• W2029861730 hasRelatedWork W3005255350 @default.
• W2029861730 hasVolume "57" @default.

• next