FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029916557> ?p ?o ?g. }

• W2029916557 endingPage "4578" @default.
• W2029916557 startingPage "4568" @default.
• W2029916557 abstract "The extracellular signal-regulated protein kinase, ERK2, fully activated by phosphorylation and without a His6 tag, shows little tendency to dimerize with or without either calcium or magnesium ions when analyzed by light scattering or analytical ultracentrifugation. Light scattering shows that ∼90% of ERK2 is monomeric. Sedimentation equilibrium data (obtained at 4.8–11.2 μM ERK2) with or without magnesium (10 mM) are well described by an ideal one-component model with a fitted molar mass of 40180 ± 240 Da (without Mg2+ ions) or 41290 ± 330 Da (with Mg2+ ions). These values, close to the sequence-derived mass of 41711 Da, indicate that no significant dimerization of ERK2 occurs in solution. Analysis of sedimentation velocity data for a 15 μM solution of ERK2 with an enhanced van Holde–Weischet method determined the sedimentation coefficient (s) to be ∼3.22 S for activated ERK2 with or without 10 mM MgCl2. The frictional coefficient ratio (f/f0) of 1.28 calculated from the sedimentation velocity and equilibrium data is close to that expected for an ∼42 kDa globular protein. The translational diffusion coefficient of ∼8.3 × 10–7 cm2 s–1 calculated from the experimentally determined molar mass and sedimentation coefficient agrees with the value determined by dynamic light scattering in the absence and presence of calcium or magnesium ions and a value determined by NMR spectrometry. ERK2 has been proposed to homodimerize and bind only to cytoplasmic but not nuclear proteins [Casar, B., et al. (2008) Mol. Cell 31, 708–721]. Our light scattering data show, however, that ERK2 forms a strong 1:1 complex of ∼57 kDa with the cytoplasmic scaffold protein PEA-15. Thus, ERK2 binds PEA-15 as a monomer. Our data provide strong evidence that ERK2 is monomeric under physiological conditions. Analysis of the same ERK2 construct with the nonphysiological His6 tag shows substantial dimerization under the same ionic conditions." @default.
• W2029916557 created "2016-06-24" @default.
• W2029916557 creator A5000319514 @default.
• W2029916557 creator A5012213782 @default.
• W2029916557 creator A5025479126 @default.
• W2029916557 creator A5048345181 @default.
• W2029916557 creator A5050439446 @default.
• W2029916557 creator A5055839723 @default.
• W2029916557 creator A5059994803 @default.
• W2029916557 creator A5079354770 @default.
• W2029916557 creator A5083510496 @default.
• W2029916557 creator A5088597944 @default.
• W2029916557 date "2011-05-04" @default.
• W2029916557 modified "2023-09-23" @default.
• W2029916557 title "Activated ERK2 Is a Monomer in Vitro with or without Divalent Cations and When Complexed to the Cytoplasmic Scaffold PEA-15" @default.
• W2029916557 cites W133296140 @default.
• W2029916557 cites W1572416479 @default.
• W2029916557 cites W1611070936 @default.
• W2029916557 cites W1639582946 @default.
• W2029916557 cites W1969294711 @default.
• W2029916557 cites W1971317954 @default.
• W2029916557 cites W1971977518 @default.
• W2029916557 cites W1975639729 @default.
• W2029916557 cites W1977891917 @default.
• W2029916557 cites W1979336952 @default.
• W2029916557 cites W1988772505 @default.
• W2029916557 cites W1992843538 @default.
• W2029916557 cites W1999517105 @default.
• W2029916557 cites W2000082154 @default.
• W2029916557 cites W2006591686 @default.
• W2029916557 cites W2008766026 @default.
• W2029916557 cites W2010218425 @default.
• W2029916557 cites W2019746426 @default.
• W2029916557 cites W2034171722 @default.
• W2029916557 cites W2036373102 @default.
• W2029916557 cites W2038447145 @default.
• W2029916557 cites W2045401053 @default.
• W2029916557 cites W2047563811 @default.
• W2029916557 cites W2048967679 @default.
• W2029916557 cites W2051171906 @default.
• W2029916557 cites W2053810673 @default.
• W2029916557 cites W2054596398 @default.
• W2029916557 cites W2068496571 @default.
• W2029916557 cites W2070658593 @default.
• W2029916557 cites W2072644702 @default.
• W2029916557 cites W2075082022 @default.
• W2029916557 cites W2076674954 @default.
• W2029916557 cites W2078834086 @default.
• W2029916557 cites W2109261059 @default.
• W2029916557 cites W2115148022 @default.
• W2029916557 cites W2126577131 @default.
• W2029916557 cites W2140490890 @default.
• W2029916557 cites W2150290925 @default.
• W2029916557 cites W2163449711 @default.
• W2029916557 cites W2317592745 @default.
• W2029916557 cites W4211258630 @default.
• W2029916557 cites W4238932755 @default.
• W2029916557 doi "https://doi.org/10.1021/bi200202y" @default.
• W2029916557 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3401516" @default.
• W2029916557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21506533" @default.
• W2029916557 hasPublicationYear "2011" @default.
• W2029916557 type Work @default.
• W2029916557 sameAs 2029916557 @default.
• W2029916557 citedByCount "37" @default.
• W2029916557 countsByYear W20299165572012 @default.
• W2029916557 countsByYear W20299165572013 @default.
• W2029916557 countsByYear W20299165572014 @default.
• W2029916557 countsByYear W20299165572015 @default.
• W2029916557 countsByYear W20299165572016 @default.
• W2029916557 countsByYear W20299165572017 @default.
• W2029916557 countsByYear W20299165572018 @default.
• W2029916557 countsByYear W20299165572019 @default.
• W2029916557 countsByYear W20299165572020 @default.
• W2029916557 countsByYear W20299165572021 @default.
• W2029916557 countsByYear W20299165572022 @default.
• W2029916557 countsByYear W20299165572023 @default.
• W2029916557 crossrefType "journal-article" @default.
• W2029916557 hasAuthorship W2029916557A5000319514 @default.
• W2029916557 hasAuthorship W2029916557A5012213782 @default.
• W2029916557 hasAuthorship W2029916557A5025479126 @default.
• W2029916557 hasAuthorship W2029916557A5048345181 @default.
• W2029916557 hasAuthorship W2029916557A5050439446 @default.
• W2029916557 hasAuthorship W2029916557A5055839723 @default.
• W2029916557 hasAuthorship W2029916557A5059994803 @default.
• W2029916557 hasAuthorship W2029916557A5079354770 @default.
• W2029916557 hasAuthorship W2029916557A5083510496 @default.
• W2029916557 hasAuthorship W2029916557A5088597944 @default.
• W2029916557 hasBestOaLocation W20299165572 @default.
• W2029916557 hasConcept C113196181 @default.
• W2029916557 hasConcept C120456961 @default.
• W2029916557 hasConcept C120665830 @default.
• W2029916557 hasConcept C121332964 @default.
• W2029916557 hasConcept C12554922 @default.
• W2029916557 hasConcept C1388644 @default.
• W2029916557 hasConcept C145148216 @default.
• W2029916557 hasConcept C166940927 @default.
• W2029916557 hasConcept C178790620 @default.
• W2029916557 hasConcept C181199279 @default.

• next