FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029943684> ?p ?o ?g. }

• W2029943684 endingPage "445" @default.
• W2029943684 startingPage "436" @default.
• W2029943684 abstract "In ciliated airway epithelial cells, purinergic stimulation increases both intracellular calcium ([Ca(2+)](i)) and ciliary beat frequency (CBF). Because regulator of G-protein signaling protein 2 (RGS2) terminates Galphaq-mediated phospholipase C activation, we examined its role in regulating purinergic signaling in human and ovine airway epithelial cells. RT-PCR of both human and ovine epithelial cell RNA yielded fragments of expected size ( approximately 491 bp) and sequence, confirming RGS2 message. Immunofluorescence demonstrated RGS2 protein expression in cultured airway epithelial cells of both species. Overexpression of an EGFP-RGS2 fusion protein (increasing RGS2 protein levels 1.8 times control, n = 28 cells) resulted in a reduced [Ca(2+)](i) and CBF response to 10 micro M ATP (human: 58 +/- 9% and 49 +/- 8% lower, respectively; n = 8 measurements, 4 cells; ovine: 56 +/- 12% and 53 +/- 16% lower, respectively; n = 5 measurements, 4 cells). Reducing RGS2 protein levels using antisense oligonucleotides increased the response of both [Ca(2+)](i) and CBF to ATP in human cells by 57 +/- 10% and 47 +/- 11%, respectively (n = 10 measurements, 6 cells), and in ovine cells by 88 +/- 13% and 48 +/- 9%, respectively (n = 10 measurements, 5 cells). These data provide functional evidence that RGS2 modulates purinergic signaling in human and ovine ciliated airway epithelial cells." @default.
• W2029943684 created "2016-06-24" @default.
• W2029943684 creator A5002808336 @default.
• W2029943684 creator A5020479008 @default.
• W2029943684 creator A5026801051 @default.
• W2029943684 creator A5086469706 @default.
• W2029943684 date "2002-10-01" @default.
• W2029943684 modified "2023-10-15" @default.
• W2029943684 title "Regulator of G-Protein Signaling Protein 2 Modulates Purinergic Calcium and Ciliary Beat Frequency Responses in Airway Epithelia" @default.
• W2029943684 cites W1531738833 @default.
• W2029943684 cites W1549603061 @default.
• W2029943684 cites W1601653849 @default.
• W2029943684 cites W1614895539 @default.
• W2029943684 cites W1908634953 @default.
• W2029943684 cites W1968536472 @default.
• W2029943684 cites W1970820951 @default.
• W2029943684 cites W1973279585 @default.
• W2029943684 cites W1977493444 @default.
• W2029943684 cites W1983971263 @default.
• W2029943684 cites W1993011200 @default.
• W2029943684 cites W1993485378 @default.
• W2029943684 cites W1997093700 @default.
• W2029943684 cites W2004288362 @default.
• W2029943684 cites W2010327392 @default.
• W2029943684 cites W2018013006 @default.
• W2029943684 cites W2022634662 @default.
• W2029943684 cites W2029283186 @default.
• W2029943684 cites W2038312257 @default.
• W2029943684 cites W2039167005 @default.
• W2029943684 cites W2050307742 @default.
• W2029943684 cites W2055518824 @default.
• W2029943684 cites W2056395380 @default.
• W2029943684 cites W2061944559 @default.
• W2029943684 cites W2068432279 @default.
• W2029943684 cites W2071230707 @default.
• W2029943684 cites W2087714421 @default.
• W2029943684 cites W2091216990 @default.
• W2029943684 cites W2092975281 @default.
• W2029943684 cites W2095013577 @default.
• W2029943684 cites W2119527238 @default.
• W2029943684 cites W2146386420 @default.
• W2029943684 cites W2149658463 @default.
• W2029943684 cites W2154457032 @default.
• W2029943684 cites W2179336653 @default.
• W2029943684 cites W4237030338 @default.
• W2029943684 cites W4294216491 @default.
• W2029943684 doi "https://doi.org/10.1165/rcmb.2002-0012oc" @default.
• W2029943684 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12356577" @default.
• W2029943684 hasPublicationYear "2002" @default.
• W2029943684 type Work @default.
• W2029943684 sameAs 2029943684 @default.
• W2029943684 citedByCount "71" @default.
• W2029943684 countsByYear W20299436842012 @default.
• W2029943684 countsByYear W20299436842013 @default.
• W2029943684 countsByYear W20299436842014 @default.
• W2029943684 countsByYear W20299436842015 @default.
• W2029943684 countsByYear W20299436842016 @default.
• W2029943684 countsByYear W20299436842017 @default.
• W2029943684 countsByYear W20299436842018 @default.
• W2029943684 countsByYear W20299436842019 @default.
• W2029943684 countsByYear W20299436842020 @default.
• W2029943684 countsByYear W20299436842021 @default.
• W2029943684 countsByYear W20299436842022 @default.
• W2029943684 countsByYear W20299436842023 @default.
• W2029943684 crossrefType "journal-article" @default.
• W2029943684 hasAuthorship W2029943684A5002808336 @default.
• W2029943684 hasAuthorship W2029943684A5020479008 @default.
• W2029943684 hasAuthorship W2029943684A5026801051 @default.
• W2029943684 hasAuthorship W2029943684A5086469706 @default.
• W2029943684 hasConcept C128240485 @default.
• W2029943684 hasConcept C153911025 @default.
• W2029943684 hasConcept C163181125 @default.
• W2029943684 hasConcept C181080969 @default.
• W2029943684 hasConcept C185592680 @default.
• W2029943684 hasConcept C2777993845 @default.
• W2029943684 hasConcept C28406088 @default.
• W2029943684 hasConcept C4963665 @default.
• W2029943684 hasConcept C54355233 @default.
• W2029943684 hasConcept C57306754 @default.
• W2029943684 hasConcept C62478195 @default.
• W2029943684 hasConcept C79879829 @default.
• W2029943684 hasConcept C80631254 @default.
• W2029943684 hasConcept C81885089 @default.
• W2029943684 hasConcept C86803240 @default.
• W2029943684 hasConcept C95444343 @default.
• W2029943684 hasConceptScore W2029943684C128240485 @default.
• W2029943684 hasConceptScore W2029943684C153911025 @default.
• W2029943684 hasConceptScore W2029943684C163181125 @default.
• W2029943684 hasConceptScore W2029943684C181080969 @default.
• W2029943684 hasConceptScore W2029943684C185592680 @default.
• W2029943684 hasConceptScore W2029943684C2777993845 @default.
• W2029943684 hasConceptScore W2029943684C28406088 @default.
• W2029943684 hasConceptScore W2029943684C4963665 @default.
• W2029943684 hasConceptScore W2029943684C54355233 @default.
• W2029943684 hasConceptScore W2029943684C57306754 @default.
• W2029943684 hasConceptScore W2029943684C62478195 @default.
• W2029943684 hasConceptScore W2029943684C79879829 @default.
• W2029943684 hasConceptScore W2029943684C80631254 @default.

• next