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• W2029995786 abstract "Polylactic acid is receiving increasing attention as a renewable alternative for conventional petroleum-based plastics. In the present study, we constructed a metabolically-engineered Candida utilis strain that produces L-lactic acid with the highest efficiency yet reported in yeasts. Initially, the gene encoding pyruvate decarboxylase (CuPDC1) was identified, followed by four CuPDC1 disruption events in order to obtain a null mutant that produced little ethanol (a by-product of L-lactic acid). Two copies of the L-lactate dehydrogenase (L-LDH) gene derived from Bos taurus under the control of the CuPDC1 promoter were then integrated into the genome of the CuPdc1-null deletant. The resulting strain produced 103.3 g/l of L-lactic acid from 108.7 g/l of glucose in 33 h, representing a 95.1% conversion. The maximum production rate of L-lactic acid was 4.9 g/l/h. The optical purity of the L-lactic acid was found to be more than 99.9% e.e." @default.
• W2029995786 created "2016-06-24" @default.
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• W2029995786 date "2009-08-01" @default.
• W2029995786 modified "2023-10-14" @default.
• W2029995786 title "Genetic engineering of Candida utilis yeast for efficient production of L-lactic acid." @default.
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• W2029995786 doi "https://doi.org/10.1271/bbb.90186" @default.
• W2029995786 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19661682" @default.
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