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- W2030011419 abstract "For analyzing protein function, protein dynamics, or protein-protein interactions, the use of chimeric proteins has become an indispensable tool. The generation of DNA constructs coding for such fused proteins can, however, be a tedious process. Currently used strategies often make use of available endonuclease sites, leading to limitations in the choice of the site of fusion between two genes and problems in maintaining protein secondary structure. We have developed a cloning strategy to get around these disadvantages that is based on a single round of PCR amplification followed by antibiotic-resistant gene complementation." @default.
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- W2030011419 date "2008-09-01" @default.
- W2030011419 modified "2023-09-28" @default.
- W2030011419 title "A PCR amplification strategy for unrestricted generation of chimeric genes" @default.
- W2030011419 cites W2083399142 @default.
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- W2030011419 doi "https://doi.org/10.1016/j.ab.2008.05.031" @default.
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