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- W2030093670 abstract "Poly G, poly IG, ribosomal RNA and poly U exhibit species-characteristic interactions with neither, one, or both strands of denatured DNA. Only one of the two complementary strands of DNA extracted from Bacillus and Clostridium species or T-odd coliphages forms complexes with ribosomal RNA, poly G or poly IG. Using CsCl density-gradient centrifugation, preparative separation of the lighter strand (dNG′) from the denser poly IG complex (DEG) containing the complementary strand is possible with the DNA of these micro-organisms. In the case of E. coli and other Enterobacteriaceae, band separation is less pronounced, since both strands complex with poly IG, albeit to various extents. Poly G is more efficient than poly IG or ribosomal RNA in effecting fractionation of the DNA strands into two widely separated bands in the CsCl gradient. The intact complementary strands of T1 and T7 coliphage DNA are separated in the presence of poly IG, indicating that cytosine-rich sequences are prevalent on only one of the two DNA strands. With the DNA of some species there is no strand separation into two distinct bands, since poly IG either reacts with both strands to similar extents (e.g. Sarcina lutea or Micrococcus lysodeikticus DNA), or reacts hardly at all (e.g. DNA from Cytophaga johnsonii or from T-even coliphage DNA). Poly U reacts with both strands of denatured DNA extracted from most of the organisms tested, with the exception of Micrococcus lysodeikticus, Sarcina lutea and coliphages T3 and T7. Every DNA tested exhibited affinity for at least one of the ribopolymers. These results seem to indicate that the mRNA-transcribing DNA strand contains widely spaced pyrimidine-rich sequences which act as the initiation points for the polycistronic RNA messages. At neutral pH, poly C, poly A and poly I do not interact with DNA. Poly C, however, interferes with the formation of complexes between denatured DNA and poly G, poly IG or ribosomal RNA, whereas poly A prevents similar DNA interaction with poly U. Complexes between denatured DNA and polynucleotides exhibit various degrees of resistance to pancreatic and T1 RNases." @default.
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- W2030093670 date "1966-09-01" @default.
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- W2030093670 title "Patterns of interaction between polyribonucleotides and individual DNA strands derived from several vertebrates, bacteria and bacteriophages" @default.
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- W2030093670 doi "https://doi.org/10.1016/0022-2836(66)90067-2" @default.
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