FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030104105> ?p ?o ?g. }

• W2030104105 endingPage "2096" @default.
• W2030104105 startingPage "2089" @default.
• W2030104105 abstract "As a powerful genome‐editing tool, the clustered regularly interspaced short palindromic repeats ( CRISPR )‐clustered regularly interspaced short palindromic repeats‐associated protein 9 (Cas9) system has been quickly developed into a large‐scale function‐based screening strategy in mammalian cells. This new type of genetic library is constructed through the lentiviral delivery of single‐guide RNA collections that direct Cas9 or inactive dead Cas9 fused with effectors to interrogate gene function or regulate gene transcription in targeted cells. Compared with RNA interference screening, the CRISPR ‐Cas9 system demonstrates much higher levels of effectiveness and reliability with respect to both loss‐of‐function and gain‐of‐function screening. Unlike the RNA interference strategy, a CRISPR ‐Cas9 library can target both protein‐coding sequences and regulatory elements, including promoters, enhancers and elements transcribing micro RNA s and long noncoding RNA s. This powerful genetic tool will undoubtedly accelerate the mechanistic discovery of various biological processes. In this mini review, we summarize the general procedure of CRISPR ‐Cas9 library mediated functional screening, system optimization strategies and applications of this new genetic toolkit." @default.
• W2030104105 created "2016-06-24" @default.
• W2030104105 creator A5000426136 @default.
• W2030104105 creator A5042991124 @default.
• W2030104105 creator A5061738324 @default.
• W2030104105 creator A5063631480 @default.
• W2030104105 date "2015-03-16" @default.
• W2030104105 modified "2023-10-14" @default.
• W2030104105 title "High‐throughput screens in mammalian cells using the CRISPR‐Cas9 system" @default.
• W2030104105 cites W147510647 @default.
• W2030104105 cites W1906155566 @default.
• W2030104105 cites W1965440903 @default.
• W2030104105 cites W1968342623 @default.
• W2030104105 cites W1973260880 @default.
• W2030104105 cites W1982846895 @default.
• W2030104105 cites W1982873976 @default.
• W2030104105 cites W1982969769 @default.
• W2030104105 cites W1991883934 @default.
• W2030104105 cites W2003171404 @default.
• W2030104105 cites W2003787610 @default.
• W2030104105 cites W2020055579 @default.
• W2030104105 cites W2031211461 @default.
• W2030104105 cites W2035420256 @default.
• W2030104105 cites W2036176224 @default.
• W2030104105 cites W2045435533 @default.
• W2030104105 cites W2064815984 @default.
• W2030104105 cites W2068427957 @default.
• W2030104105 cites W2073873559 @default.
• W2030104105 cites W2084481314 @default.
• W2030104105 cites W2085146326 @default.
• W2030104105 cites W2090787765 @default.
• W2030104105 cites W2092806325 @default.
• W2030104105 cites W2095560152 @default.
• W2030104105 cites W2100122648 @default.
• W2030104105 cites W2101749257 @default.
• W2030104105 cites W2112444085 @default.
• W2030104105 cites W2123746851 @default.
• W2030104105 cites W2127862779 @default.
• W2030104105 cites W2129063117 @default.
• W2030104105 cites W2130410032 @default.
• W2030104105 cites W2131395804 @default.
• W2030104105 cites W2133102806 @default.
• W2030104105 cites W2142437744 @default.
• W2030104105 cites W2144654038 @default.
• W2030104105 cites W2152239989 @default.
• W2030104105 cites W2152505144 @default.
• W2030104105 cites W2153344788 @default.
• W2030104105 cites W2160444236 @default.
• W2030104105 doi "https://doi.org/10.1111/febs.13251" @default.
• W2030104105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25731961" @default.
• W2030104105 hasPublicationYear "2015" @default.
• W2030104105 type Work @default.
• W2030104105 sameAs 2030104105 @default.
• W2030104105 citedByCount "50" @default.
• W2030104105 countsByYear W20301041052016 @default.
• W2030104105 countsByYear W20301041052017 @default.
• W2030104105 countsByYear W20301041052018 @default.
• W2030104105 countsByYear W20301041052019 @default.
• W2030104105 countsByYear W20301041052020 @default.
• W2030104105 countsByYear W20301041052021 @default.
• W2030104105 countsByYear W20301041052022 @default.
• W2030104105 crossrefType "journal-article" @default.
• W2030104105 hasAuthorship W2030104105A5000426136 @default.
• W2030104105 hasAuthorship W2030104105A5042991124 @default.
• W2030104105 hasAuthorship W2030104105A5061738324 @default.
• W2030104105 hasAuthorship W2030104105A5063631480 @default.
• W2030104105 hasConcept C104317684 @default.
• W2030104105 hasConcept C111936080 @default.
• W2030104105 hasConcept C132455925 @default.
• W2030104105 hasConcept C138885662 @default.
• W2030104105 hasConcept C144501496 @default.
• W2030104105 hasConcept C166703698 @default.
• W2030104105 hasConcept C179926584 @default.
• W2030104105 hasConcept C41895202 @default.
• W2030104105 hasConcept C51785407 @default.
• W2030104105 hasConcept C54355233 @default.
• W2030104105 hasConcept C67705224 @default.
• W2030104105 hasConcept C70721500 @default.
• W2030104105 hasConcept C86339819 @default.
• W2030104105 hasConcept C86803240 @default.
• W2030104105 hasConcept C95444343 @default.
• W2030104105 hasConcept C97702854 @default.
• W2030104105 hasConcept C98108389 @default.
• W2030104105 hasConceptScore W2030104105C104317684 @default.
• W2030104105 hasConceptScore W2030104105C111936080 @default.
• W2030104105 hasConceptScore W2030104105C132455925 @default.
• W2030104105 hasConceptScore W2030104105C138885662 @default.
• W2030104105 hasConceptScore W2030104105C144501496 @default.
• W2030104105 hasConceptScore W2030104105C166703698 @default.
• W2030104105 hasConceptScore W2030104105C179926584 @default.
• W2030104105 hasConceptScore W2030104105C41895202 @default.
• W2030104105 hasConceptScore W2030104105C51785407 @default.
• W2030104105 hasConceptScore W2030104105C54355233 @default.
• W2030104105 hasConceptScore W2030104105C67705224 @default.
• W2030104105 hasConceptScore W2030104105C70721500 @default.
• W2030104105 hasConceptScore W2030104105C86339819 @default.
• W2030104105 hasConceptScore W2030104105C86803240 @default.
• W2030104105 hasConceptScore W2030104105C95444343 @default.

• next