FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030112760> ?p ?o ?g. }

• W2030112760 endingPage "97" @default.
• W2030112760 startingPage "92" @default.
• W2030112760 abstract "Epidermal growth factor (EGF) has been shown to be a potent mitogen for vascular smooth muscle cells (VSMC) both in vitro and in vivo, thus contributing to the development of atherosclerosis and hypertension. Stimulation of Rho-family GTPases Rac/Cdc42 exerts pleiotropic cellular effects and have been demonstrated to contribute to EGF-induced proliferation in other cell systems. However, the effect of EGF on Rac/Cdc42 activation is unknown for VSMC. In the present report, we evaluated stimulation of Rac/Cdc42 by EGF in VSMC performing PAK-PBD binding assay. EGF treatment of VSMC induced time and concentration dependent binding of GTP-bound Rac1 to PAK-PBD peaking at 1 min and showing sustained activation up to 15 min. However, stimulation of Cdc42 could not be demonstrated. To further evaluate downstream effectors of Rac1 stimulation of p21-activated kinase (PAK) and c-Jun N-terminal kinase (JNK) by EGF was determined. In VSMC, EGF sequentially stimulated PAK, peaking at 5 min, and JNK, peaking at 15 min. Pretreatment of VSMC by EGF receptor specific tyrosine kinase inhibitor AG1478 and non-specific tyrosine kinase inhibitor genistein inhibited EGF-induced activation of Rac1, PAK and JNK, whereas tyrosine kinase inhibitors specific for Src (PP1) and specific for platelet-derived growth factor (AG1296) had no effect. Specific inhibition or Rac1 by NSC23766 attenuated EGF-induced [(3)H] thymidine incorporation in VSMC. Our data provide evidence for EGF-induced Rac1 activation and implicate PAK and JNK as downstream targets of Rac1 in EGF signal transduction in VSMC." @default.
• W2030112760 created "2016-06-24" @default.
• W2030112760 creator A5000764134 @default.
• W2030112760 creator A5055002837 @default.
• W2030112760 creator A5058239108 @default.
• W2030112760 creator A5086901004 @default.
• W2030112760 date "2008-01-01" @default.
• W2030112760 modified "2023-09-24" @default.
• W2030112760 title "Epidermal growth factor stimulates Rac1 and p21-activated kinase in vascular smooth muscle cells" @default.
• W2030112760 cites W1969586748 @default.
• W2030112760 cites W1973891472 @default.
• W2030112760 cites W1978977283 @default.
• W2030112760 cites W1994531989 @default.
• W2030112760 cites W2011756230 @default.
• W2030112760 cites W2029705406 @default.
• W2030112760 cites W2052780342 @default.
• W2030112760 cites W2055181868 @default.
• W2030112760 cites W2056507417 @default.
• W2030112760 cites W2080145523 @default.
• W2030112760 cites W2082817039 @default.
• W2030112760 cites W2096791952 @default.
• W2030112760 cites W2102014126 @default.
• W2030112760 cites W2106906257 @default.
• W2030112760 cites W2119081725 @default.
• W2030112760 cites W2125856024 @default.
• W2030112760 cites W2127730758 @default.
• W2030112760 cites W2128915459 @default.
• W2030112760 cites W2155764594 @default.
• W2030112760 cites W2158973316 @default.
• W2030112760 cites W2158974373 @default.
• W2030112760 doi "https://doi.org/10.1016/j.atherosclerosis.2007.02.003" @default.
• W2030112760 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17350025" @default.
• W2030112760 hasPublicationYear "2008" @default.
• W2030112760 type Work @default.
• W2030112760 sameAs 2030112760 @default.
• W2030112760 citedByCount "28" @default.
• W2030112760 countsByYear W20301127602012 @default.
• W2030112760 countsByYear W20301127602013 @default.
• W2030112760 countsByYear W20301127602014 @default.
• W2030112760 countsByYear W20301127602015 @default.
• W2030112760 countsByYear W20301127602016 @default.
• W2030112760 countsByYear W20301127602020 @default.
• W2030112760 countsByYear W20301127602021 @default.
• W2030112760 countsByYear W20301127602022 @default.
• W2030112760 crossrefType "journal-article" @default.
• W2030112760 hasAuthorship W2030112760A5000764134 @default.
• W2030112760 hasAuthorship W2030112760A5055002837 @default.
• W2030112760 hasAuthorship W2030112760A5058239108 @default.
• W2030112760 hasAuthorship W2030112760A5086901004 @default.
• W2030112760 hasConcept C108636557 @default.
• W2030112760 hasConcept C134018914 @default.
• W2030112760 hasConcept C170493617 @default.
• W2030112760 hasConcept C180361614 @default.
• W2030112760 hasConcept C185592680 @default.
• W2030112760 hasConcept C2775960820 @default.
• W2030112760 hasConcept C2776362946 @default.
• W2030112760 hasConcept C2779395532 @default.
• W2030112760 hasConcept C2779717556 @default.
• W2030112760 hasConcept C2780040266 @default.
• W2030112760 hasConcept C2992686903 @default.
• W2030112760 hasConcept C42362537 @default.
• W2030112760 hasConcept C55493867 @default.
• W2030112760 hasConcept C62478195 @default.
• W2030112760 hasConcept C86803240 @default.
• W2030112760 hasConcept C95444343 @default.
• W2030112760 hasConceptScore W2030112760C108636557 @default.
• W2030112760 hasConceptScore W2030112760C134018914 @default.
• W2030112760 hasConceptScore W2030112760C170493617 @default.
• W2030112760 hasConceptScore W2030112760C180361614 @default.
• W2030112760 hasConceptScore W2030112760C185592680 @default.
• W2030112760 hasConceptScore W2030112760C2775960820 @default.
• W2030112760 hasConceptScore W2030112760C2776362946 @default.
• W2030112760 hasConceptScore W2030112760C2779395532 @default.
• W2030112760 hasConceptScore W2030112760C2779717556 @default.
• W2030112760 hasConceptScore W2030112760C2780040266 @default.
• W2030112760 hasConceptScore W2030112760C2992686903 @default.
• W2030112760 hasConceptScore W2030112760C42362537 @default.
• W2030112760 hasConceptScore W2030112760C55493867 @default.
• W2030112760 hasConceptScore W2030112760C62478195 @default.
• W2030112760 hasConceptScore W2030112760C86803240 @default.
• W2030112760 hasConceptScore W2030112760C95444343 @default.
• W2030112760 hasIssue "1" @default.
• W2030112760 hasLocation W20301127601 @default.
• W2030112760 hasLocation W20301127602 @default.
• W2030112760 hasOpenAccess W2030112760 @default.
• W2030112760 hasPrimaryLocation W20301127601 @default.
• W2030112760 hasRelatedWork W138305676 @default.
• W2030112760 hasRelatedWork W1993982469 @default.
• W2030112760 hasRelatedWork W1999064050 @default.
• W2030112760 hasRelatedWork W2030112760 @default.
• W2030112760 hasRelatedWork W2038595692 @default.
• W2030112760 hasRelatedWork W2051751591 @default.
• W2030112760 hasRelatedWork W2055810659 @default.
• W2030112760 hasRelatedWork W2278549878 @default.
• W2030112760 hasRelatedWork W2469155503 @default.
• W2030112760 hasRelatedWork W2759236970 @default.
• W2030112760 hasVolume "196" @default.
• W2030112760 isParatext "false" @default.

• next