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- W2030146796 abstract "Investigation of the genotoxic potential of nanomaterials is essential to evaluate if they pose a cancer risk for exposed workers and consumers. The Chinese hamster ovary cell line CHO-K1 is recommended by the OECD for use in the micronucleus assay and is commonly used for genotoxicity testing. However, studies investigating if this cell line is suitable for the genotoxic evaluation of nanomaterials, including induction of DNA adduct and micronuclei formation, are rare and for silver nanoparticles (Ag NPs) missing. Therefore, we here systematically investigated DNA and chromosomal damage induced by BSA coated Ag NPs (15.9±7.6 nm) in CHO-K1 cells in relation to cellular uptake and intracellular localization, their effects on mitochondrial activity and production of reactive oxygen species (ROS), cell cycle, apoptosis and necrosis. Ag NPs are taken up by CHO-K1 cells and are presumably translocated into endosomes/lysosomes. Our cytotoxicity studies demonstrated a concentration-dependent decrease of mitochondrial activity and increase of intracellular reactive oxygen species (ROS) in CHO-K1 cells following exposure to Ag NPs and Ag⁺ (0-20 μg/ml) for 24h. Annexin V/propidium iodide assay showed that Ag NPs and Ag⁺ induced apoptosis and necrosis, which is in agreement with an increased fraction of cells in subG1 phase of the cell cycle. Genotoxicity studies showed that Ag NPs but also silver ions (Ag⁺) induced bulky-DNA adducts, 8-oxodG and micronuclei formation in a concentration-dependent manner, however, there were quantitative and qualitative differences between the particulate and ionic form of silver. Taken together, our multi-platform genotoxicity and cytotoxicity analysis demonstrates that CHO-K1 cells are suitable for the investigation of genotoxicity of nanoparticles like Ag NPs." @default.
- W2030146796 created "2016-06-24" @default.
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- W2030146796 date "2013-09-01" @default.
- W2030146796 modified "2023-10-17" @default.
- W2030146796 title "Multi-platform genotoxicity analysis of silver nanoparticles in the model cell line CHO-K1" @default.
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- W2030146796 cites W1973240521 @default.
- W2030146796 cites W1973253352 @default.
- W2030146796 cites W1981125126 @default.
- W2030146796 cites W1993373375 @default.
- W2030146796 cites W1996366132 @default.
- W2030146796 cites W2003336829 @default.
- W2030146796 cites W2008154812 @default.
- W2030146796 cites W2008583081 @default.
- W2030146796 cites W2009792330 @default.
- W2030146796 cites W2010226025 @default.
- W2030146796 cites W2010303079 @default.
- W2030146796 cites W2010615178 @default.
- W2030146796 cites W2010966520 @default.
- W2030146796 cites W2015780991 @default.
- W2030146796 cites W2033473289 @default.
- W2030146796 cites W2034069830 @default.
- W2030146796 cites W2037299114 @default.
- W2030146796 cites W2037426867 @default.
- W2030146796 cites W2039843548 @default.
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- W2030146796 cites W2066431692 @default.
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- W2030146796 cites W2071850731 @default.
- W2030146796 cites W2073286303 @default.
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- W2030146796 cites W2092214136 @default.
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- W2030146796 doi "https://doi.org/10.1016/j.toxlet.2013.07.011" @default.
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