FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030176793> ?p ?o ?g. }

• W2030176793 endingPage "407" @default.
• W2030176793 startingPage "397" @default.
• W2030176793 abstract "We report the combined use of real-time photo-CIDNP NMR and stopped-flow fluorescence techniques to study the kinetic refolding of a set of mutants of a small globular protein, HPr, in which each of the four phenylalanine residues has in turn been replaced by a tryptophan residue. The results indicate that after refolding is initiated, the protein collapses around at least three, and possibly all four, of the side-chains of these residues, as (i) the observation of transient histidine photo-CIDNP signals during refolding of three of the mutants (F2W, F29W, and F48W) indicates a strong decrease in tryptophan accessibility to the flavin dye; (ii) iodide quenching experiments show that the quenching of the fluorescence of F48W is less efficient for the species formed during the dead-time of the stopped-flow experiment than for the fully native state; and (iii) kinetic fluorescence anisotropy measurements show that the tryptophan side-chain of F48W has lower mobility in the dead-time intermediate state than in both the fully denatured and fully native states. The hydrophobic collapse observed for HPr during the early stages of its folding appears to act primarily to bury hydrophobic residues. This process may be important in preventing the protein from aggregating prior to the acquisition of native-like structure in which hydrophobic residues are exposed in order to play their role in the function of the protein. The phenylalanine residue at position 48 is likely to be of particular interest in this regard as it is involved in the binding to enzymes I and II that mediates the transfer of a phosphoryl group between the two enzymes." @default.
• W2030176793 created "2016-06-24" @default.
• W2030176793 creator A5016518886 @default.
• W2030176793 creator A5023344535 @default.
• W2030176793 creator A5025215567 @default.
• W2030176793 creator A5025765239 @default.
• W2030176793 creator A5060479290 @default.
• W2030176793 creator A5083811779 @default.
• W2030176793 creator A5091898546 @default.
• W2030176793 date "2003-07-01" @default.
• W2030176793 modified "2023-09-23" @default.
• W2030176793 title "Rapid Formation of Non-native Contacts During the Folding of HPr Revealed by Real-time Photo-CIDNP NMR and Stopped-flow Fluorescence Experiments" @default.
• W2030176793 cites W1977263087 @default.
• W2030176793 cites W1978033776 @default.
• W2030176793 cites W1984332944 @default.
• W2030176793 cites W1996518756 @default.
• W2030176793 cites W1996919359 @default.
• W2030176793 cites W2008585649 @default.
• W2030176793 cites W2011964130 @default.
• W2030176793 cites W20120559 @default.
• W2030176793 cites W2014336356 @default.
• W2030176793 cites W2017256978 @default.
• W2030176793 cites W2024566241 @default.
• W2030176793 cites W2028231353 @default.
• W2030176793 cites W2029243412 @default.
• W2030176793 cites W203578729 @default.
• W2030176793 cites W2039913544 @default.
• W2030176793 cites W2074433337 @default.
• W2030176793 cites W2075075608 @default.
• W2030176793 cites W2076834744 @default.
• W2030176793 cites W2080763659 @default.
• W2030176793 cites W2090283830 @default.
• W2030176793 cites W2090873614 @default.
• W2030176793 cites W2133567359 @default.
• W2030176793 cites W2143971218 @default.
• W2030176793 cites W2169513577 @default.
• W2030176793 cites W2769999982 @default.
• W2030176793 cites W2952271863 @default.
• W2030176793 cites W4232547663 @default.
• W2030176793 cites W4239300697 @default.
• W2030176793 doi "https://doi.org/10.1016/s0022-2836(03)00507-2" @default.
• W2030176793 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12823977" @default.
• W2030176793 hasPublicationYear "2003" @default.
• W2030176793 type Work @default.
• W2030176793 sameAs 2030176793 @default.
• W2030176793 citedByCount "35" @default.
• W2030176793 countsByYear W20301767932012 @default.
• W2030176793 countsByYear W20301767932013 @default.
• W2030176793 countsByYear W20301767932018 @default.
• W2030176793 countsByYear W20301767932021 @default.
• W2030176793 countsByYear W20301767932022 @default.
• W2030176793 countsByYear W20301767932023 @default.
• W2030176793 crossrefType "journal-article" @default.
• W2030176793 hasAuthorship W2030176793A5016518886 @default.
• W2030176793 hasAuthorship W2030176793A5023344535 @default.
• W2030176793 hasAuthorship W2030176793A5025215567 @default.
• W2030176793 hasAuthorship W2030176793A5025765239 @default.
• W2030176793 hasAuthorship W2030176793A5060479290 @default.
• W2030176793 hasAuthorship W2030176793A5083811779 @default.
• W2030176793 hasAuthorship W2030176793A5091898546 @default.
• W2030176793 hasBestOaLocation W20301767932 @default.
• W2030176793 hasConcept C111357860 @default.
• W2030176793 hasConcept C119599485 @default.
• W2030176793 hasConcept C121332964 @default.
• W2030176793 hasConcept C121745418 @default.
• W2030176793 hasConcept C12554922 @default.
• W2030176793 hasConcept C127413603 @default.
• W2030176793 hasConcept C139066938 @default.
• W2030176793 hasConcept C178790620 @default.
• W2030176793 hasConcept C185592680 @default.
• W2030176793 hasConcept C19472624 @default.
• W2030176793 hasConcept C204328495 @default.
• W2030176793 hasConcept C204921945 @default.
• W2030176793 hasConcept C2776001701 @default.
• W2030176793 hasConcept C2776545253 @default.
• W2030176793 hasConcept C2776706248 @default.
• W2030176793 hasConcept C2777431362 @default.
• W2030176793 hasConcept C2778870691 @default.
• W2030176793 hasConcept C41625074 @default.
• W2030176793 hasConcept C47701112 @default.
• W2030176793 hasConcept C515207424 @default.
• W2030176793 hasConcept C521977710 @default.
• W2030176793 hasConcept C55493867 @default.
• W2030176793 hasConcept C62520636 @default.
• W2030176793 hasConcept C75473681 @default.
• W2030176793 hasConcept C8010536 @default.
• W2030176793 hasConcept C86803240 @default.
• W2030176793 hasConcept C91881484 @default.
• W2030176793 hasConceptScore W2030176793C111357860 @default.
• W2030176793 hasConceptScore W2030176793C119599485 @default.
• W2030176793 hasConceptScore W2030176793C121332964 @default.
• W2030176793 hasConceptScore W2030176793C121745418 @default.
• W2030176793 hasConceptScore W2030176793C12554922 @default.
• W2030176793 hasConceptScore W2030176793C127413603 @default.
• W2030176793 hasConceptScore W2030176793C139066938 @default.
• W2030176793 hasConceptScore W2030176793C178790620 @default.
• W2030176793 hasConceptScore W2030176793C185592680 @default.
• W2030176793 hasConceptScore W2030176793C19472624 @default.

• next