Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030202214> ?p ?o ?g. }
- W2030202214 endingPage "5352" @default.
- W2030202214 startingPage "5344" @default.
- W2030202214 abstract "Characterization of mono- and bis-mannose-6-phosphate (M6P) bearing oligosaccharides present on acid hydrolase enzymes poses a considerable analytical challenge. In the current paper, we investigated the use of UPLC profiling on a 1.7 μm HILIC phase and capillary electrophoresis with laser induced fluorescence detection (CE-LIF) combined with exoglycosidase digestion and weak anion exchange fractionation for the characterization of M6P bearing glycans on recombinant β-glucuronidase expressed in Chinese Hamster Ovary (CHO) cells. Using this multidimensional approach a number of peaks were observed to resist digestion, suggesting the presence and blocking activity of the M6P tag. To investigate further, mixed oxide affinity purification on a combined TiO(2)/ZrO(2) resin facilitated the selective enrichment of oligosaccharides bearing mono- or diphospho-esters that corresponded to those peaks previously identified to resist exoglycosidase digestion. Alkaline phosphatase digestion identified Man(6)GlcNAc(2) and Man(7)GlcNAc(2) glycans as the primary carriers of the M6P tag. Site-specific glycoproteomic analysis revealed that Man(7)GlcNAc(2)-M6P oligosaccharides were present at asparagine 272 and 420, while asparagine 631 displayed Man(6)GlcNAc(2)-M6P. The analytical strategy applied herein represents a novel yet simple approach for the qualitative and semiquantitative structural characterization of M6P containing oligosaccharides on therapeutic enzymes." @default.
- W2030202214 created "2016-06-24" @default.
- W2030202214 creator A5001484555 @default.
- W2030202214 creator A5020402727 @default.
- W2030202214 creator A5030947204 @default.
- W2030202214 creator A5060796671 @default.
- W2030202214 creator A5071547468 @default.
- W2030202214 creator A5082473050 @default.
- W2030202214 creator A5088540624 @default.
- W2030202214 creator A5091562783 @default.
- W2030202214 date "2011-06-07" @default.
- W2030202214 modified "2023-09-27" @default.
- W2030202214 title "Identification of <i>N</i>-Glycans Displaying Mannose-6-Phosphate and their Site of Attachment on Therapeutic Enzymes for Lysosomal Storage Disorder Treatment" @default.
- W2030202214 cites W1491723881 @default.
- W2030202214 cites W1526653134 @default.
- W2030202214 cites W1605749013 @default.
- W2030202214 cites W1972767597 @default.
- W2030202214 cites W1974960744 @default.
- W2030202214 cites W1975182596 @default.
- W2030202214 cites W1975654543 @default.
- W2030202214 cites W1985437385 @default.
- W2030202214 cites W1987063014 @default.
- W2030202214 cites W1991849437 @default.
- W2030202214 cites W1997315604 @default.
- W2030202214 cites W2015049954 @default.
- W2030202214 cites W2024824708 @default.
- W2030202214 cites W2028533348 @default.
- W2030202214 cites W2035338952 @default.
- W2030202214 cites W2039276803 @default.
- W2030202214 cites W2041337564 @default.
- W2030202214 cites W2049263258 @default.
- W2030202214 cites W2059807960 @default.
- W2030202214 cites W2060282482 @default.
- W2030202214 cites W2069274491 @default.
- W2030202214 cites W2071726169 @default.
- W2030202214 cites W2073094263 @default.
- W2030202214 cites W2082474796 @default.
- W2030202214 cites W2083270783 @default.
- W2030202214 cites W2097385470 @default.
- W2030202214 cites W2116313758 @default.
- W2030202214 cites W2116346196 @default.
- W2030202214 cites W2120403003 @default.
- W2030202214 cites W2131898019 @default.
- W2030202214 cites W2133695108 @default.
- W2030202214 cites W2136686929 @default.
- W2030202214 cites W2139601009 @default.
- W2030202214 cites W2149357832 @default.
- W2030202214 cites W2152297447 @default.
- W2030202214 cites W2169237458 @default.
- W2030202214 cites W2322128806 @default.
- W2030202214 doi "https://doi.org/10.1021/ac2007784" @default.
- W2030202214 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21599028" @default.
- W2030202214 hasPublicationYear "2011" @default.
- W2030202214 type Work @default.
- W2030202214 sameAs 2030202214 @default.
- W2030202214 citedByCount "26" @default.
- W2030202214 countsByYear W20302022142012 @default.
- W2030202214 countsByYear W20302022142013 @default.
- W2030202214 countsByYear W20302022142014 @default.
- W2030202214 countsByYear W20302022142015 @default.
- W2030202214 countsByYear W20302022142016 @default.
- W2030202214 countsByYear W20302022142017 @default.
- W2030202214 countsByYear W20302022142018 @default.
- W2030202214 countsByYear W20302022142019 @default.
- W2030202214 countsByYear W20302022142021 @default.
- W2030202214 countsByYear W20302022142022 @default.
- W2030202214 countsByYear W20302022142023 @default.
- W2030202214 crossrefType "journal-article" @default.
- W2030202214 hasAuthorship W2030202214A5001484555 @default.
- W2030202214 hasAuthorship W2030202214A5020402727 @default.
- W2030202214 hasAuthorship W2030202214A5030947204 @default.
- W2030202214 hasAuthorship W2030202214A5060796671 @default.
- W2030202214 hasAuthorship W2030202214A5071547468 @default.
- W2030202214 hasAuthorship W2030202214A5082473050 @default.
- W2030202214 hasAuthorship W2030202214A5088540624 @default.
- W2030202214 hasAuthorship W2030202214A5091562783 @default.
- W2030202214 hasConcept C108625454 @default.
- W2030202214 hasConcept C116817701 @default.
- W2030202214 hasConcept C170493617 @default.
- W2030202214 hasConcept C175656101 @default.
- W2030202214 hasConcept C179998833 @default.
- W2030202214 hasConcept C181199279 @default.
- W2030202214 hasConcept C185592680 @default.
- W2030202214 hasConcept C206212055 @default.
- W2030202214 hasConcept C2775887612 @default.
- W2030202214 hasConcept C2775960820 @default.
- W2030202214 hasConcept C2778822930 @default.
- W2030202214 hasConcept C2781178804 @default.
- W2030202214 hasConcept C43617362 @default.
- W2030202214 hasConcept C55493867 @default.
- W2030202214 hasConceptScore W2030202214C108625454 @default.
- W2030202214 hasConceptScore W2030202214C116817701 @default.
- W2030202214 hasConceptScore W2030202214C170493617 @default.
- W2030202214 hasConceptScore W2030202214C175656101 @default.
- W2030202214 hasConceptScore W2030202214C179998833 @default.
- W2030202214 hasConceptScore W2030202214C181199279 @default.
- W2030202214 hasConceptScore W2030202214C185592680 @default.
- W2030202214 hasConceptScore W2030202214C206212055 @default.