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- W2030219390 abstract "The shape and spatio-temporal development of a concentration gradient depend on the method and medium of its generation. Model gradients of low molecular weight (MW) dyes develop with predictable characteristics in 1% agarose. In contrast, any method of delivering dyes into free solution, e.g., via capillary pipets, produces anomalous gradients, unless the chambers are extremely shallow (less than 1 mm deep). Thus, most methods of chemo-attractant delivery which are popular in chemotaxis studies are incapable of propagating gradients by molecular diffusion; rather, they tend to be dominated by the effects of wholesale flow of the chemo-attractant and convection and turbulence of the free solution. This may have serious consequences for the interpretation of some chemotaxis experiments, since several gradient qualities of chemo-attractants (and probably morphogens) could influence cell behaviour. The possible effects on cells of the motion and development of gradients are also discussed." @default.
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- W2030219390 date "1981-11-01" @default.
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- W2030219390 title "Ideal and non-ideal concentration gradient propagation in chemotaxis studies" @default.
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- W2030219390 doi "https://doi.org/10.1016/0014-4827(81)90040-9" @default.
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