FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030256319> ?p ?o ?g. }

• W2030256319 endingPage "99" @default.
• W2030256319 startingPage "76" @default.
• W2030256319 abstract "The fate of the genome of the polyoma (Py) tumor virus following integration in the chromosomes of transformed rat FR3T3 cells was re-examined. The viral sequences were integrated at a single transformant-specific chromosomal site in each of 22 transformants tested. In situ amplification of the viral sequences was observed in 24 of 34 transformants analyzed. Large T antigen, the unique viral function involved in initiating DNA replication from the viral origin, was essential for the amplification process. There was an absolute requirement for a reiteration of viral sequences and the extent of the reiteration affected the degree of amplification. The reiteration may be important for homologous recombination-mediated resolution of in situ amplified sequences. Among 11 transformants harboring a 1 to 2 kb repeat, the degree of amplification was transformant-specific and varied over a wide range. At the high end of the spectrum, the genome copy number increased 1300-fold at steady state, while at the low end, amplification was below twofold. Some aspect of the host chromatin at the site integration that affected viral gene expression, also directly or indirectly modulated the amplification. Use of high-resolution electrophoresis for the analysis of the integrated amplified sequences revealed a recurring novel pattern, consisting of a ladder with numerous bands separated by a constant distance approximately the size of the Py genome. We suggest that this pattern was generated by conversion of the amplified viral genomes to head to tail linear arrays with cell to cell variations in the number of genome repeats at single, transformant-specific, chromosomal sites. In light of the known “out of schedule” firing of the Py origin, we propose an “onion skin” structure intermediate and present a homologous recombination model for the conversion from onion skins to linear arrays. The relevance of the in situ amplification of the Py genome to cellular gene amplification is discussed. Finally, these results clarify our understanding of the integration of the Py genome in rat cells. They suggest that, in most cases, the multiple bands previously described in Py-transformants are likely to reflect genome amplification rather than multiple independent integration events, as assumed in the past. This interpretation is congruent with the accepted view that the integration of the Py genome is a rare and rate-limiting event in transformation." @default.
• W2030256319 created "2016-06-24" @default.
• W2030256319 creator A5030487288 @default.
• W2030256319 creator A5078858200 @default.
• W2030256319 date "1997-08-01" @default.
• W2030256319 modified "2023-09-29" @default.
• W2030256319 title "Site-specific in Situ amplification of the integrated polyomavirus genome: A case for a context-specific over-replication model of gene amplification" @default.
• W2030256319 cites W1485023261 @default.
• W2030256319 cites W1506684772 @default.
• W2030256319 cites W1507756275 @default.
• W2030256319 cites W1535218232 @default.
• W2030256319 cites W1537951898 @default.
• W2030256319 cites W1556753125 @default.
• W2030256319 cites W1565953496 @default.
• W2030256319 cites W1574025064 @default.
• W2030256319 cites W1581372463 @default.
• W2030256319 cites W1599408992 @default.
• W2030256319 cites W1635257526 @default.
• W2030256319 cites W1694794195 @default.
• W2030256319 cites W1767451291 @default.
• W2030256319 cites W1772918310 @default.
• W2030256319 cites W1775335842 @default.
• W2030256319 cites W1955521412 @default.
• W2030256319 cites W1956466310 @default.
• W2030256319 cites W1962018562 @default.
• W2030256319 cites W1967446284 @default.
• W2030256319 cites W1970972488 @default.
• W2030256319 cites W1971235310 @default.
• W2030256319 cites W1973591990 @default.
• W2030256319 cites W1978222338 @default.
• W2030256319 cites W1989806518 @default.
• W2030256319 cites W2004891293 @default.
• W2030256319 cites W2004954672 @default.
• W2030256319 cites W2005309070 @default.
• W2030256319 cites W2008586935 @default.
• W2030256319 cites W2011376946 @default.
• W2030256319 cites W2020957952 @default.
• W2030256319 cites W2021681334 @default.
• W2030256319 cites W2027861413 @default.
• W2030256319 cites W2033013773 @default.
• W2030256319 cites W2033782571 @default.
• W2030256319 cites W2045652766 @default.
• W2030256319 cites W2046573633 @default.
• W2030256319 cites W2052160913 @default.
• W2030256319 cites W2052513192 @default.
• W2030256319 cites W2053684265 @default.
• W2030256319 cites W2056200309 @default.
• W2030256319 cites W2056694609 @default.
• W2030256319 cites W2059334666 @default.
• W2030256319 cites W2063932193 @default.
• W2030256319 cites W2065393892 @default.
• W2030256319 cites W2066967151 @default.
• W2030256319 cites W2072162346 @default.
• W2030256319 cites W2092987461 @default.
• W2030256319 cites W2118261407 @default.
• W2030256319 cites W2170398046 @default.
• W2030256319 cites W2305556775 @default.
• W2030256319 cites W2316008018 @default.
• W2030256319 cites W2317242660 @default.
• W2030256319 cites W4248545692 @default.
• W2030256319 cites W2074136481 @default.
• W2030256319 doi "https://doi.org/10.1006/jmbi.1997.1156" @default.
• W2030256319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9300056" @default.
• W2030256319 hasPublicationYear "1997" @default.
• W2030256319 type Work @default.
• W2030256319 sameAs 2030256319 @default.
• W2030256319 citedByCount "14" @default.
• W2030256319 countsByYear W20302563192014 @default.
• W2030256319 countsByYear W20302563192016 @default.
• W2030256319 countsByYear W20302563192017 @default.
• W2030256319 countsByYear W20302563192022 @default.
• W2030256319 crossrefType "journal-article" @default.
• W2030256319 hasAuthorship W2030256319A5030487288 @default.
• W2030256319 hasAuthorship W2030256319A5078858200 @default.
• W2030256319 hasConcept C102744134 @default.
• W2030256319 hasConcept C104317684 @default.
• W2030256319 hasConcept C140704245 @default.
• W2030256319 hasConcept C141231307 @default.
• W2030256319 hasConcept C153911025 @default.
• W2030256319 hasConcept C2522874641 @default.
• W2030256319 hasConcept C54355233 @default.
• W2030256319 hasConcept C552990157 @default.
• W2030256319 hasConcept C86803240 @default.
• W2030256319 hasConceptScore W2030256319C102744134 @default.
• W2030256319 hasConceptScore W2030256319C104317684 @default.
• W2030256319 hasConceptScore W2030256319C140704245 @default.
• W2030256319 hasConceptScore W2030256319C141231307 @default.
• W2030256319 hasConceptScore W2030256319C153911025 @default.
• W2030256319 hasConceptScore W2030256319C2522874641 @default.
• W2030256319 hasConceptScore W2030256319C54355233 @default.
• W2030256319 hasConceptScore W2030256319C552990157 @default.
• W2030256319 hasConceptScore W2030256319C86803240 @default.
• W2030256319 hasIssue "1" @default.
• W2030256319 hasLocation W20302563191 @default.
• W2030256319 hasLocation W20302563192 @default.
• W2030256319 hasOpenAccess W2030256319 @default.
• W2030256319 hasPrimaryLocation W20302563191 @default.
• W2030256319 hasRelatedWork W1991523530 @default.

• next