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- W2030289564 abstract "The expression, purification, crystallization, and characterization by X-ray diffraction of α-amylase are described here. Dynamic and static light scattering methods with a temperature controller was used to optimize the crystallization conditions of α-amylase from Bacillus stearothermophilus an important enzyme in many fields of industrial activity. After applying thermal gradients for growing crystals, X-ray cryo-crystallographic methods were employed for the data collection. Crystals grown by these thermal-gradients diffracted up to a maximum resolution of 3.8 Å, which allowed the determination of the unit cell constants as follows: a=61.7Å, b=86.7Å, c=92.2Å and space group C222 (or C2221)." @default.
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- W2030289564 date "2002-02-01" @default.
- W2030289564 modified "2023-10-14" @default.
- W2030289564 title "Applications of thermal-gradients method for the optimization of α-amylase crystallization conditions based on dynamic and static light scattering data" @default.
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- W2030289564 doi "https://doi.org/10.1016/s0022-2860(01)00663-9" @default.
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